FastQCFastQC Report
Sun 1 Oct 2023
SRR7471310.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471310.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11020066
Total Bases562 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT122662211.130804479755383No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT8618627.820842452304733No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTA6937896.295688247239173No Hit
ACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCG3543903.2158609576385477No Hit
ATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATGCC2314772.100504661224352No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTAT1737681.5768326614377808No Hit
TTTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATG1552671.4089480044856355No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTA1542091.3993473360322888No Hit
ATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGTAT1530711.3890207191136605No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGCCGT1448921.314801562894451No Hit
TTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATGC1374261.247052422372062No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGT1198671.0877158085986054No Hit
CAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA1180941.071626975736806No Hit
TGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATG1029960.9346223516265693No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCG930100.8440058344478154No Hit
AGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTA829550.7527631867177564No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTAT776860.7049504059231587No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTATG734690.6666838474470116No Hit
TCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATG651750.5914211403089601No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCG626580.5685809867200432No Hit
TGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATGCC579830.52615837328016No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCGT547290.4966304194548381No Hit
TCCAACGGGGCCTTTCCTTCTGGCCTGTAGGCACCATCAATTCGTATGCC525760.4770933313829518No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATGC480100.43565982272701453No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTATG473490.42966167353262674No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA425560.38616828610645343Illumina Single End Adapter 1 (95% over 22bp)
TTTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGT414290.37594148710180136No Hit
TCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGC386860.35105052909846457No Hit
TGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGT356900.32386375907367526No Hit
TTTGGAAGAGATGTATTTATTAGATCTGTAGGCACCATCAATTCGTATGC337840.30656803688834533No Hit
TCCAACGGGGCCTTTCCTTCTGGCTCTGTAGGCACCATCAATTCGTATGC328790.29835574487484917No Hit
ACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTATGCCGTC313230.2842360472251255No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGC301900.2739548020855773No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATTCGTATG299920.2721580796340058No Hit
TACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATG294450.2671944070026441No Hit
AAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA287520.26090587842214374No Hit
TCAATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCG285980.259508427626477No Hit
TTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTAT270260.24524354028369702No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA233200.2116139776295351No Hit
TTGGAAGAGATGTATTTATTAGATAACTGTAGGCACCATCAATTCGTATG231670.21022560118968436No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCCTGTAGGCACCATCAATTCGT215850.19586997028874417No Hit
AAGAGGTGCACAATCGACCGATCCTGATCTGTAGGCACCATCAATTCGTA213240.19350156342076355No Hit
ATGTATTTATTAGATAAAAAATCAATCTGTAGGCACCATCAATTCGTATG213150.19341989421841937No Hit
TTTGGAAGAGATGTATTTATTAGATAACTGTAGGCACCATCAATTCGTAT199480.18101524981792305No Hit
TAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA197590.1793001965686957No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTATG177070.16067961843422718No Hit
TGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCGT174840.1586560370872552No Hit
AGAGATGTATTTATTAGATAAAAAATCCTGTAGGCACCATCAATTCGTAT170470.15469054359565543No Hit
CTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT165670.15033485280396686No Hit
TAGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGT159860.14506265207486052No Hit
AGAGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTC157730.14312981428604873No Hit
AAGAGGTGCACAATCGACCGATCCTCTGTAGGCACCATCAATTCGTATGC147740.13406453282584696No Hit
TTGGAAGAGATGTATTTATTAGATCTGTAGGCACCATCAATTCGTATGCC145020.13159630804389014No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATTCGT138030.1252533333284937No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCTCTGTAGGCACCATCAATTCG135800.12322975198152172No Hit
TGATGTATTTATTAGATAAAAAATCCTGTAGGCACCATCAATTCGTATGC130000.11796662560823137No Hit
GTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT129830.11781236155935908No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATTCGT126400.11469985751446497No Hit
TTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATG124570.11303925040013373No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTA120700.10952747469933483No Hit
TCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCGT118100.10716814218717019No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCCTGTAGGCACCATCAATTCGTA116010.10527160182162247No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)