FastQCFastQC Report
Sun 1 Oct 2023
SRR7471311.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471311.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11762638
Total Bases599.8 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA6558955.5760876089190194Illumina Single End Adapter 1 (95% over 22bp)
TCCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATTCGT2522512.1445104406001443No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCTGTAGGCACCATCAATTC1340051.1392427446972355No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAAT1312571.115880638339801No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT1282821.090588692774529No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGCTGTAGGCACCATCAATTCG927250.7883010596772594No Hit
TTGATGTTGACGCAATGTGATTTCTGCCCACTGTAGGCACCATCAATTCG730350.6209066367595433No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATTCG640760.5447417492572669No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATTC621060.5279938054711877No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATTCG591990.5032799615188361No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCG551990.46927398428821837No Hit
TACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATG538030.45740589823473277No Hit
ACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCG497270.42275380743673313No Hit
ATGCCCTGACGAGTAGGCAGGCGTGGAGGTCACTGTAGGCACCATCAATT448930.3816575839535315No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCG445560.37879258037185193No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAGCTGTAGGCACCATCAA441060.3749669079334075No Hit
TTGCCCGCGCAGGTTCGAGTCCTGCAGTTGTCGCTGTAGGCACCATCAAT421780.3585760269082497No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTA410610.34907985776659967No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCG394480.335366947448353No Hit
AGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTA379340.3224956850665641No Hit
GTCTAGTCGGTTATGGCATCTGCTCTGTAGGCACCATCAATTCGTATGCC377010.32051483689288063No Hit
TCCAACGGGGCCTTTCCTTCTGGCCTGTAGGCACCATCAATTCGTATGCC368180.3130080174192218No Hit
AGCGTGTTACCTATACTCTACCGTCAGGGTCTGTAGGCACCATCAATTCG352140.29937162054974403No Hit
TAGCGTGTTACCTATACTCTACCGTCAGGGTCTGTAGGCACCATCAATTC351580.2988955368685154No Hit
TTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATTCGTATGCC347240.2952058883389933No Hit
AAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATTCGTATGCCGT344680.29302950579623377No Hit
TTTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATTCGTATGC344550.29291898637023434No Hit
AAGCGTGTTACCTATACTCTACCGTCAGGGTCTGTAGGCACCATCAATTC326000.2771487144295353No Hit
ATGGCCTCTTCGAAGATCCGTAGATTTTCTGTAGGCACCATCAATTCGTA311800.26507659251266597No Hit
GTTTAGTGGTAAAATCCAACGTCTGTAGGCACCATCAATTCGTATGCCGT304490.25886200017377053No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGCTGTAGGCACCATCAATT299230.25439021416794433No Hit
TGCTGGCTCCGGTGCGCTTGTGACGGCCCTGTAGGCACCATCAATTCGTA288510.24527661227013872No Hit
TTGCCCTGACGAGTAGGCAGGCGTGGAGGTCACTGTAGGCACCATCAATT283320.24086433672446608No Hit
TGCTGGCTCCGGTGCGCTTGTGACGGCCTGTAGGCACCATCAATTCGTAT276190.23480277128310845No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTA275610.23430968461326446No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA273980.2329239410411168No Hit
TTGCCCGCGCAGGTTCGAGTCCTGCAGTTGCTGTAGGCACCATCAATTCG261120.22199101936147317No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT258750.21997616521055907No Hit
ATGCCCTGACGAGTAGGCAGGCGTGGAGGTCCTGTAGGCACCATCAATTC255890.21754473783856987No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCGCTGTAGGCACCATCAATTC250300.21279240252059106No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA228180.1939870971120594Illumina Single End Adapter 1 (95% over 22bp)
TTTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATG223500.19000839777607711No Hit
ATGGCCTCTTCGAAGATCCGTAGATTTTGCACTGTAGGCACCATCAATTC218330.18561312521901976No Hit
TTTGGAAGAGATGTATTTATTAGATAACTGTAGGCACCATCAATTCGTAT216890.1843889100387175No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATGC213090.18115834220180882No Hit
TATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTA213070.1811413392131935No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGGCTGTAGGCACCATCAATTC211050.1794240373630473No Hit
TTTGCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAA201270.17110957593016124No Hit
TTGGCCCTGATGATAATGGTGTCTCTGTAGGCACCATCAATTCGTATGCC199080.16924774867678494No Hit
CCGGAGATGGGGTCTTATGGCTGGCTGTAGGCACCATCAATTCGTATGCC196520.16707136613402537No Hit
ACGTAGAGTTAAGGTGCCGGAATACACGCTCTGTAGGCACCATCAATTCG195970.1666037839471044No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGGCTGTAGGCACCATCAATTCGT195730.16639974808372068No Hit
TTGGGTAAAACCATACTGCAGACATCTCTGTAGGCACCATCAATTCGTAT194360.165235043363572No Hit
TTACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTAT192830.16393431473450087No Hit
TTGCCCTGACGAGTAGGCAGGCGTGGAGGTCCTGTAGGCACCATCAATTC187050.1590204510246766No Hit
TAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATTCGTATGCCG181800.154557166513158No Hit
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA179500.15260182282239748Illumina Single End Adapter 1 (95% over 22bp)
TGATGTTGACGCAATGTGATTTCTGCCCACTGTAGGCACCATCAATTCGT174890.14868263394656878No Hit
TTGGAAGAGATGTATTTATTAGATAACTGTAGGCACCATCAATTCGTATG174390.14825755923118605No Hit
TCCGGAGATGGGGTCTTATGGCTGGCTGTAGGCACCATCAATTCGTATGC174130.14803652037918705No Hit
TTGCGAGAGGCCCTGGGTTCATTTCCCAGCTCGCCCCTGTAGGCACCATC171430.14574111691612035No Hit
AGGTGGGGACACGGGTTCGATTCTCGTACCGCCTGTAGGCACCATCAATT171130.1454860720868907No Hit
TTTAGATTCTCTTCACGCACATACCTGTAGGCACCATCAATTCGTATGCC166950.14193244746629113No Hit
TTGGGGACACGGGTTCGATTCTCGTACCGCCTGTAGGCACCATCAATTCG156240.13282734706279323No Hit
ATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATTCGTATGC154100.13100802728095517No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTAT153570.1305574480826495No Hit
TTGATGTTGACGCAATGTGATTTCTGCCCTGTAGGCACCATCAATTCGTA151770.12902717910727168No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCTGTAGGCACCATCAATTCGTA151770.12902717910727168No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGT150650.1280750117448144No Hit
TTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATGC150190.12768394300666228No Hit
ATGGCCTCTTCGAAGATCCGTAGATTTTGCCTGTAGGCACCATCAATTCG142050.12076372664023155No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGCCGT140780.11968403686315944No Hit
TCGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCTGTAGGCACCATCAAT136820.11631744511732826No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCCTGTAGGCACCATCAATT134020.11393702671118502No Hit
TCCGATGTAGTGTAACGGCTATCACATCACGCTGTAGGCACCATCAATTC132360.11252577865611439No Hit
ATTAGATTCTCTTCACGCACATACCTGTAGGCACCATCAATTCGTATGCC130780.11118254255550498No Hit
TTGGCCCTGATGATAATGGTGTCTCCTGTAGGCACCATCAATTCGTATGC130260.11074046485150696No Hit
ATTGCACAACTTAAGTTTGTCGAGGATCTGTAGGCACCATCAATTCGTAT128860.10955025564843533No Hit
TGCTGGCTCCGGTGCGCTTGTGACGGCTGTAGGCACCATCAATTCGTATG123970.1053930249319923No Hit
TTTGCTGGCTCCGGTGCGCTTGTGACGGCCCTGTAGGCACCATCAATTCG123520.10501045768814785No Hit
TTGCGAGAGGCCCTGGGTTCATTTCCCAGCTCGCCCTGTAGGCACCATCA122740.1043473411321508No Hit
TTGCTGGCTCCGGTGCGCTTGTGACGGCCCTGTAGGCACCATCAATTCGT121890.10362471411600017No Hit
AACTGTTGGTGCTGAGGTAATCCATCTTTCTGTAGGCACCATCAATTCGT121340.10315713192907917No Hit
TCGGGCTTGGCAGAATCAGCGGGGACTGTAGGCACCATCAATTCGTATGC120590.10251951985600508No Hit
ATCAAACAGTTATCCCTGTCTGAATGGGTACTGTAGGCACCATCAATTCG119260.10138882111308704No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)