FastQCFastQC Report
Sun 1 Oct 2023
SRR7471312.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471312.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11738303
Total Bases598.6 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA6572445.599139841593797Illumina Single End Adapter 1 (95% over 22bp)
TCCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATTCGT2506852.135615344057825No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCTGTAGGCACCATCAATTC1341721.143027233152867No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAAT1312871.1184495748661454No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT1287351.09670878320316No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGCTGTAGGCACCATCAATTCG916440.78072614073772No Hit
TTGATGTTGACGCAATGTGATTTCTGCCCACTGTAGGCACCATCAATTCG733300.6247069955512309No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATTCG635480.5413729735891125No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATTC617980.5264645153562657No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATTCG596170.5078843168386435No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCG548300.4671032942325649No Hit
TACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATG537720.4580900663409353No Hit
ACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCG494160.4209807840196322No Hit
ATGCCCTGACGAGTAGGCAGGCGTGGAGGTCACTGTAGGCACCATCAATT450490.3837777913894368No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCG441640.3762383710831114No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAGCTGTAGGCACCATCAA441500.37611910341724863No Hit
TTGCCCGCGCAGGTTCGAGTCCTGCAGTTGTCGCTGTAGGCACCATCAAT418570.3565847635727243No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTA407700.3473244812303789No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCG397560.3386860945743179No Hit
AGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTA381400.32491919828615773No Hit
GTCTAGTCGGTTATGGCATCTGCTCTGTAGGCACCATCAATTCGTATGCC374970.31944140477546035No Hit
TCCAACGGGGCCTTTCCTTCTGGCCTGTAGGCACCATCAATTCGTATGCC366400.3121405198008605No Hit
AGCGTGTTACCTATACTCTACCGTCAGGGTCTGTAGGCACCATCAATTCG356180.30343398019287793No Hit
TAGCGTGTTACCTATACTCTACCGTCAGGGTCTGTAGGCACCATCAATTC352470.30027338704751444No Hit
AAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATTCGTATGCCGT347310.2958775216485722No Hit
TTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATTCGTATGCC347270.2958434451726114No Hit
TTTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATTCGTATGC346240.294965975916621No Hit
AAGCGTGTTACCTATACTCTACCGTCAGGGTCTGTAGGCACCATCAATTC324490.27643689211294No Hit
ATGGCCTCTTCGAAGATCCGTAGATTTTCTGTAGGCACCATCAATTCGTA312970.2666228670362317No Hit
GTTTAGTGGTAAAATCCAACGTCTGTAGGCACCATCAATTCGTATGCCGT300580.2560676786073762No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGCTGTAGGCACCATCAATT297430.2533841561254638No Hit
TGCTGGCTCCGGTGCGCTTGTGACGGCCCTGTAGGCACCATCAATTCGTA291630.24844306711114886No Hit
TTGCCCTGACGAGTAGGCAGGCGTGGAGGTCACTGTAGGCACCATCAATT284400.2422837440812356No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTA277120.23608182545637132No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA276760.2357751371727242No Hit
TGCTGGCTCCGGTGCGCTTGTGACGGCCTGTAGGCACCATCAATTCGTAT275700.2348721105597632No Hit
TTGCCCGCGCAGGTTCGAGTCCTGCAGTTGCTGTAGGCACCATCAATTCG259960.2214630172691913No Hit
ATGCCCTGACGAGTAGGCAGGCGTGGAGGTCCTGTAGGCACCATCAATTC258210.2199721714459066No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT257380.2192650845697202No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCGCTGTAGGCACCATCAATTC248690.21186197016723798No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA227730.19400589676378263Illumina Single End Adapter 1 (95% over 22bp)
TTTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATG225250.1918931552542135No Hit
TTTGGAAGAGATGTATTTATTAGATAACTGTAGGCACCATCAATTCGTAT218040.18575087046228062No Hit
ATGGCCTCTTCGAAGATCCGTAGATTTTGCACTGTAGGCACCATCAATTC213980.18229210815226018No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATGC212960.18142315801525996No Hit
TATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTA211780.18041790197441657No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGGCTGTAGGCACCATCAATTC211490.18017084752370083No Hit
TTGGCCCTGATGATAATGGTGTCTCTGTAGGCACCATCAATTCGTATGCC199790.17020347830516896No Hit
TTTGCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAA199000.16953046790494333No Hit
ACGTAGAGTTAAGGTGCCGGAATACACGCTCTGTAGGCACCATCAATTCG198210.16885745750471767No Hit
TTGGGTAAAACCATACTGCAGACATCTCTGTAGGCACCATCAATTCGTAT193820.16511756426802068No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGGCTGTAGGCACCATCAATTCGT193150.1645467832956774No Hit
CCGGAGATGGGGTCTTATGGCTGGCTGTAGGCACCATCAATTCGTATGCC192880.16431676708294204No Hit
TTACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTAT192330.16384821553848114No Hit
TTGCCCTGACGAGTAGGCAGGCGTGGAGGTCCTGTAGGCACCATCAATTC186900.15922233392680354No Hit
TAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATTCGTATGCCG181570.15468164350502794No Hit
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA176780.15060098550872303Illumina Single End Adapter 1 (95% over 22bp)
TCCGGAGATGGGGTCTTATGGCTGGCTGTAGGCACCATCAATTCGTATGC173550.14784931007488902No Hit
TTGCGAGAGGCCCTGGGTTCATTTCCCAGCTCGCCCCTGTAGGCACCATC173310.14764485121912424No Hit
TGATGTTGACGCAATGTGATTTCTGCCCACTGTAGGCACCATCAATTCGT172490.146946283461928No Hit
AGGTGGGGACACGGGTTCGATTCTCGTACCGCCTGTAGGCACCATCAATT171060.14572804944632967No Hit
TTGGAAGAGATGTATTTATTAGATAACTGTAGGCACCATCAATTCGTATG170120.14492725226125105No Hit
TTTAGATTCTCTTCACGCACATACCTGTAGGCACCATCAATTCGTATGCC167280.14250782246803476No Hit
TTGGGGACACGGGTTCGATTCTCGTACCGCCTGTAGGCACCATCAATTCG157520.13419316233360137No Hit
ATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATTCGTATGC154830.13190151932523805No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTAT154390.13152667808966934No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCTGTAGGCACCATCAATTCGTA151600.12914984389140405No Hit
TTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATGC150980.12862165851401178No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGT149550.12740342449841344No Hit
TTGATGTTGACGCAATGTGATTTCTGCCCTGTAGGCACCATCAATTCGTA148850.12680708616909958No Hit
ATGGCCTCTTCGAAGATCCGTAGATTTTGCCTGTAGGCACCATCAATTCG143470.12222380015237296No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGCCGT143270.12205341777256899No Hit
TCGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCTGTAGGCACCATCAAT137660.11727419201906783No Hit
TCCGATGTAGTGTAACGGCTATCACATCACGCTGTAGGCACCATCAATTC133090.11338095464054727No Hit
TTGGCCCTGATGATAATGGTGTCTCCTGTAGGCACCATCAATTCGTATGC132860.11318501490377272No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCCTGTAGGCACCATCAATT132320.11272498247830202No Hit
ATTGCACAACTTAAGTTTGTCGAGGATCTGTAGGCACCATCAATTCGTAT130930.11154082493866448No Hit
ATTAGATTCTCTTCACGCACATACCTGTAGGCACCATCAATTCGTATGCC130150.11087633365742902No Hit
TGCTGGCTCCGGTGCGCTTGTGACGGCTGTAGGCACCATCAATTCGTATG126060.10739201399043796No Hit
TTGCGAGAGGCCCTGGGTTCATTTCCCAGCTCGCCCTGTAGGCACCATCA122380.10425697820204505No Hit
TTGCTGGCTCCGGTGCGCTTGTGACGGCCCTGTAGGCACCATCAATTCGT121980.10391621344243714No Hit
TTTGCTGGCTCCGGTGCGCTTGTGACGGCCCTGTAGGCACCATCAATTCG121180.10323468392322127No Hit
TCGGGCTTGGCAGAATCAGCGGGGACTGTAGGCACCATCAATTCGTATGC120690.10281724709270157No Hit
AACTGTTGGTGCTGAGGTAATCCATCTTTCTGTAGGCACCATCAATTCGT120240.10243388673814265No Hit
AGCGACTCTTCTTCATGATAAGACAGTTCTGTAGGCACCATCAATTCGTA118520.10096859827182857No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)