FastQCFastQC Report
Sun 1 Oct 2023
SRR7471315.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471315.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12306693
Total Bases627.6 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA222456518.076058287957615Illumina Single End Adapter 1 (95% over 22bp)
TCCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATTCGT1467851.1927249668127742No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAAT1056300.8583134396868435No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA827420.672333339265065Illumina Single End Adapter 1 (95% over 22bp)
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT757670.6156568624893787No Hit
ACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCG721580.5863313564415721No Hit
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA697760.5669760349104346Illumina Single End Adapter 1 (95% over 22bp)
TCCGATATAGTGTAACGGCTATCACATCACGCTGTAGGCACCATCAATTC624290.5072768127067118No Hit
TTGATGTTGACGCAATGTGATTTCTGCCCACTGTAGGCACCATCAATTCG587090.47704935842634577No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGCTGTAGGCACCATCAATTCG570790.46380453302930363No Hit
TACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATG522670.4247038582988948No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA501470.40747745962298726No Hit
AAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATTCGTATGCCGT440840.3582115845418424No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAGCTGTAGGCACCATCAA426850.346843786547694No Hit
AGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTA425270.34555993230675375No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATTCG387000.3144630324328396No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCG363240.29515646486021874No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTA358630.2914105357141841No Hit
TTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATTCGTATGCC350500.2848043743351687No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATTCG348420.28311423710658906No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCG346350.28143222553776226No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGCCGT341970.27787318656604176No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATGC338450.2750129543330609No Hit
TGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA320330.2602892588610116Illumina Single End Adapter 1 (95% over 22bp)
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATTC305820.24849892655971836No Hit
TTGCCCGCGCAGGTTCGAGTCCTGCAGTTGTCGCTGTAGGCACCATCAAT296460.24089330903111014No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCG295080.2397719679852256No Hit
ATGCCCTGACGAGTAGGCAGGCGTGGAGGTCACTGTAGGCACCATCAATT287970.23399462390099438No Hit
TTTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATTCGTATGC287760.23382398504618582No Hit
TGCTGGCTCCGGTGCGCTTGTGACGGCCCTGTAGGCACCATCAATTCGTA282840.22982616044781487No Hit
GTCTAGTCGGTTATGGCATCTGCTCTGTAGGCACCATCAATTCGTATGCC274290.22287872135918238No Hit
TGCTGGCTCCGGTGCGCTTGTGACGGCCTGTAGGCACCATCAATTCGTAT255920.20795188439331347No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT238040.1934232047553311No Hit
TATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTA225740.18342864325940364No Hit
ATCAAACAGTTATCCCTGTCTGAATGGGTACTGTAGGCACCATCAATTCG213670.17362097193779027No Hit
TAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATTCGTATGCCG209960.17060635216950645No Hit
TTTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATG205670.16712044413556104No Hit
AGCGTGTTACCTATACTCTACCGTCAGGGTCTGTAGGCACCATCAATTCG199440.16205815810957502No Hit
TTGCCCGCGCAGGTTCGAGTCCTGCAGTTGCTGTAGGCACCATCAATTCG198320.16114808421726293No Hit
AAGCGTGTTACCTATACTCTACCGTCAGGGTCTGTAGGCACCATCAATTC197810.1607336755698708No Hit
ATGCCCTGACGAGTAGGCAGGCGTGGAGGTCCTGTAGGCACCATCAATTC197240.16027051296396197No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTA195750.15905978966079679No Hit
TCCAACGGGGCCTTTCCTTCTGGCCTGTAGGCACCATCAATTCGTATGCC194480.15802783087219288No Hit
TCCAACGGGGCCTTTCCTTCTGGCTCTGTAGGCACCATCAATTCGTATGC193220.15700399774334178No Hit
TTTGGAAGAGATGTATTTATTAGATAACTGTAGGCACCATCAATTCGTAT190260.15459880245651694No Hit
ATGGCCTCTTCGAAGATCCGTAGATTTTCTGTAGGCACCATCAATTCGTA189040.15360747196667698No Hit
TAGCGTGTTACCTATACTCTACCGTCAGGGTCTGTAGGCACCATCAATTC186400.15146229779194134No Hit
TTGCCCTGACGAGTAGGCAGGCGTGGAGGTCACTGTAGGCACCATCAATT179270.14566870238820454No Hit
TTACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTAT176800.14366166442926626No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT176510.14342602029643545Illumina Single End Adapter 1 (95% over 22bp)
GTTTAGTGGTAAAATCCAACGTCTGTAGGCACCATCAATTCGTATGCCGT175570.14266220827967352No Hit
AGGTGGGGACACGGGTTCGATTCTCGTACCGCCTGTAGGCACCATCAATT172440.14011887677705132No Hit
CCGGAGATGGGGTCTTATGGCTGGCTGTAGGCACCATCAATTCGTATGCC169810.13798182826206845No Hit
CTGTAGGCACATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA167060.13574727183005214Illumina Single End Adapter 1 (95% over 22bp)
TTCCGTGATAGTTTAATGGTCAGAATGGGCGCTGTAGGCACCATCAATTC163810.13310643241039652No Hit
TGATGTTGACGCAATGTGATTTCTGCCCACTGTAGGCACCATCAATTCGT162340.1319119604267369No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGT159470.12957989607768716No Hit
GTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATTCGTATGCCGTC159380.12950676513991208No Hit
TTTGCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAA157130.1276784916955351No Hit
TTGGAAGAGATGTATTTATTAGATAACTGTAGGCACCATCAATTCGTATG149310.12132422576885601No Hit
TGCTGGCTCCGGTGCGCTTGTGACGGCTGTAGGCACCATCAATTCGTATG149190.12122671785182257No Hit
ACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA148170.12039790055703836Illumina Single End Adapter 1 (95% over 22bp)
TTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA146430.11898403576005348Illumina Single End Adapter 1 (95% over 22bp)
TTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATGC145700.11839086259810008No Hit
TCCGGAGATGGGGTCTTATGGCTGGCTGTAGGCACCATCAATTCGTATGC144960.11778956377639387No Hit
GTCTAGTGGTATGATTCTCGCTCTGTAGGCACCATCAATTCGTATGCCGT141140.1146855617508294No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTAT136900.11124028201564791No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGCTGTAGGCACCATCAATT135740.11029770548432467No Hit
TTGCCCTGACGAGTAGGCAGGCGTGGAGGTCCTGTAGGCACCATCAATTC135130.10980204023940468No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGGCTGTAGGCACCATCAATTC133770.10869695051302572No Hit
TTGCGAGAGGCCCTGGGTTCATTTCCCAGCTCGCCCCTGTAGGCACCATC133690.10863194523500343No Hit
ATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATTCGTATGC130860.10633238352496482No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGGCTGTAGGCACCATCAATTCGT130210.1058042156410337No Hit
ATGGCCTCTTCGAAGATCCGTAGATTTTGCACTGTAGGCACCATCAATTC127040.10322838149940036No Hit
AAACATAGCATTGCGATGGTCACTGTAGGCACCATCAATTCGTATGCCGT126220.10256207739967188No Hit
TCAAACAGTTATCCCTGTCTGAATGGGTACTGTAGGCACCATCAATTCGT123220.1001243794738359No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)