FastQCFastQC Report
Sun 1 Oct 2023
SRR7471316.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471316.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12343997
Total Bases629.5 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA222214018.00178661741412Illumina Single End Adapter 1 (95% over 22bp)
TCCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATTCGT1478491.1977400837022238No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAAT1056430.8558249001518714No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA833180.6749677596324756Illumina Single End Adapter 1 (95% over 22bp)
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT763810.6187704031360345No Hit
ACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCG723910.5864469992985255No Hit
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA693300.5616495208156644Illumina Single End Adapter 1 (95% over 22bp)
TCCGATATAGTGTAACGGCTATCACATCACGCTGTAGGCACCATCAATTC622510.5043018075911717No Hit
TTGATGTTGACGCAATGTGATTTCTGCCCACTGTAGGCACCATCAATTCG586580.47519454192997623No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGCTGTAGGCACCATCAATTCG577720.4680169640352311No Hit
TACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATG525410.4256400904828477No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA504190.40844954839182157No Hit
AAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATTCGTATGCCGT436480.35359697511268023No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAGCTGTAGGCACCATCAA428400.34705128330799173No Hit
AGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTA423890.34339768553086975No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATTCG388930.31507622693038567No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCG366740.29709987777864816No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTA356680.2889501674376622No Hit
TTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATTCGTATGCC349940.28349002353127595No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCG349280.2829553506858435No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGCCGT346450.28066273833345873No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATTCG342060.2771063538009609No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATGC341640.2767661074447766No Hit
TGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA314770.2549984417526997Illumina Single End Adapter 1 (95% over 22bp)
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATTC310680.25168509033176206No Hit
TTGCCCGCGCAGGTTCGAGTCCTGCAGTTGTCGCTGTAGGCACCATCAAT298760.24202857469910274No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCG297880.2413156775718594No Hit
TTTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATTCGTATGC288800.2339598753953035No Hit
ATGCCCTGACGAGTAGGCAGGCGTGGAGGTCACTGTAGGCACCATCAATT287040.23253408114081686No Hit
TGCTGGCTCCGGTGCGCTTGTGACGGCCCTGTAGGCACCATCAATTCGTA280280.2270577350269933No Hit
GTCTAGTCGGTTATGGCATCTGCTCTGTAGGCACCATCAATTCGTATGCC276370.22389020347299177No Hit
TGCTGGCTCCGGTGCGCTTGTGACGGCCTGTAGGCACCATCAATTCGTAT256930.2081416578438896No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT238650.19333284024615366No Hit
TATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTA229530.18594463365472302No Hit
ATCAAACAGTTATCCCTGTCTGAATGGGTACTGTAGGCACCATCAATTCG214510.17377677586927476No Hit
TAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATTCGTATGCCG211030.17095759177517622No Hit
TTTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATG205720.16665590570056035No Hit
ATGCCCTGACGAGTAGGCAGGCGTGGAGGTCCTGTAGGCACCATCAATTC200400.16234611852222583No Hit
AGCGTGTTACCTATACTCTACCGTCAGGGTCTGTAGGCACCATCAATTCG198200.16056387570411756No Hit
TTGCCCGCGCAGGTTCGAGTCCTGCAGTTGCTGTAGGCACCATCAATTCG198160.16053147128924286No Hit
TCCAACGGGGCCTTTCCTTCTGGCCTGTAGGCACCATCAATTCGTATGCC196600.15926769910912972No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTA195240.15816594900339007No Hit
AAGCGTGTTACCTATACTCTACCGTCAGGGTCTGTAGGCACCATCAATTC194640.15767988278026962No Hit
TCCAACGGGGCCTTTCCTTCTGGCTCTGTAGGCACCATCAATTCGTATGC191890.1554520792576343No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT189900.15383995961761818Illumina Single End Adapter 1 (95% over 22bp)
ATGGCCTCTTCGAAGATCCGTAGATTTTCTGTAGGCACCATCAATTCGTA189510.1535240165725899No Hit
TAGCGTGTTACCTATACTCTACCGTCAGGGTCTGTAGGCACCATCAATTC187760.15210632342182195No Hit
TTTGGAAGAGATGTATTTATTAGATAACTGTAGGCACCATCAATTCGTAT187640.15200911017719787No Hit
TTGCCCTGACGAGTAGGCAGGCGTGGAGGTCACTGTAGGCACCATCAATT178340.1444750837188311No Hit
TTACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTAT176540.1430168850494698No Hit
GTTTAGTGGTAAAATCCAACGTCTGTAGGCACCATCAATTCGTATGCCGT175430.142117662536697No Hit
CCGGAGATGGGGTCTTATGGCTGGCTGTAGGCACCATCAATTCGTATGCC171940.13929037733887978No Hit
AGGTGGGGACACGGGTTCGATTCTCGTACCGCCTGTAGGCACCATCAATT171680.13907974864219425No Hit
CTGTAGGCACATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA163890.13276898884534727Illumina Single End Adapter 1 (95% over 22bp)
GTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATTCGTATGCCGTC161990.1312297791387992No Hit
TGATGTTGACGCAATGTGATTTCTGCCCACTGTAGGCACCATCAATTCGT161730.13101915044211368No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCGCTGTAGGCACCATCAATTC161500.1308328250565842No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGT156660.126911890856746No Hit
TTTGCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAA154740.12535647894276059No Hit
TGCTGGCTCCGGTGCGCTTGTGACGGCTGTAGGCACCATCAATTCGTATG148770.12052012002271226No Hit
TTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATGC147380.11939406660581657No Hit
TTGGAAGAGATGTATTTATTAGATAACTGTAGGCACCATCAATTCGTATG147300.11932925777606719No Hit
TTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA146090.11834902422610763Illumina Single End Adapter 1 (95% over 22bp)
ACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA144800.1173039818463987Illumina Single End Adapter 1 (95% over 22bp)
TCCGGAGATGGGGTCTTATGGCTGGCTGTAGGCACCATCAATTCGTATGC144560.11710955535715054No Hit
GTCTAGTGGTATGATTCTCGCTCTGTAGGCACCATCAATTCGTATGCCGT138710.11237040968172626No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGCTGTAGGCACCATCAATT137250.11118764853879988No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTAT137060.11103372756814506No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGGCTGTAGGCACCATCAATTC132880.10764746621373936No Hit
TTGCGAGAGGCCCTGGGTTCATTTCCCAGCTCGCCCCTGTAGGCACCATC132700.10750164634680322No Hit
ATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATTCGTATGC131690.10668343487121716No Hit
TTGCCCTGACGAGTAGGCAGGCGTGGAGGTCCTGTAGGCACCATCAATTC130680.10586522339563109No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGGCTGTAGGCACCATCAATTCGT129950.1052738428241679No Hit
ATGGCCTCTTCGAAGATCCGTAGATTTTGCACTGTAGGCACCATCAATTC128150.10381564415480658No Hit
AAACATAGCATTGCGATGGTCACTGTAGGCACCATCAATTCGTATGCCGT126380.10238174879660128No Hit
TCAAACAGTTATCCCTGTCTGAATGGGTACTGTAGGCACCATCAATTCGT124550.10089924681608398No Hit
TTGGGTAAAACCATACTGCAGACATCTCTGTAGGCACCATCAATTCGTAT123990.10044558500783823No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC123740.10024305741487137Illumina Single End Adapter 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)