FastQCFastQC Report
Fri 7 Jul 2023
SRR7513745.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7513745.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53606378
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length61
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA18334783.4202609249220304No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT7520171.4028498623801817No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA7002121.3062102423707864No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAGAT5868471.0947335408484415No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAGA3880530.7238933397067043No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAATAGATCG3859740.7200150698485915No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGATC3706540.6914363809470583No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG3496510.6522563415868164No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCCTGTAGGCACCATCAATAGA3392590.6328705886452541No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCGG3191670.5953899739318332No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA2892600.5395999707348257No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAATAGATCGG2840430.5298679198210332No Hit
ATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGATCGGAAGAGCA2822600.5265418230644122No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG2702440.504126579863314No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT2597270.48450764571335153No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA2457990.4585256627485632No Hit
GGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGATCG2377180.4434509639879046No Hit
GCGGTCGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT2220570.4142361567498554Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCCTGTAGGCACCATCAATAG2109120.39344572020889007No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG2069320.38602123053342646No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCCTGTAGGCACCATCAATAGAT1960580.36573633085227286No Hit
ATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCAC1928850.3598172590582412No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGG1859730.34692327095854153No Hit
GGGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGAT1796380.3351056473168174No Hit
TCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCAC1784310.3328540495685047No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATAGATC1782410.33249961413173634No Hit
AAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATAGATCGGAAGAG1772280.3306099136188608No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATA1768350.32987679189965047No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATAGATCG1726760.3221183867337577No Hit
AGCGGTCGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1683720.31408949136612063No Hit
TATCAGATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC1660960.30984372792356907Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAAT1601390.2987312442560473No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCTGTAGGCACCATCAATAGATCGG1519340.2834252297366556No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG1476790.27548774140271143No Hit
CCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATAGATCGGAA1463080.2729302099089776No Hit
ATGCGTGCATTTATCAGATCCTGTAGGCACCATCAATAGATCGGAAGAGC1412740.26353953628428317No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCTGTAGGCACCATCAATA1410900.263196293545518No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCG1410800.26317763904884606No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCCTGTAGGCACCATCAATAGAT1404500.2620024057585088No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG1397970.2607842671258259No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC1331230.2483342560469204No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAATAGATC1313020.2449372722029457No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA1312440.24482907612224802No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAGATC1295020.24157946280198225No Hit
GGGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATC1293990.24138732148626046No Hit
GTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGGAAG1270530.2370109765670048No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAGA1258580.23478176421469849No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCCTGTAGGCACCATCAAT1203300.2244695584544063No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAGATCG1197370.22336334680175557No Hit
TATCAGATCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA1190620.22210416827639426Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGGGGATGCGTGCATTTATCAGATCCTGTAGGCACCATCAATAGATC1178210.2197891452393967No Hit
GATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGATCGGAAGAGC1172520.21872770437875882No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCTGTAGGCACCATCAATAGATCGGA1101740.20552405163430368No Hit
TCTGATCGAGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1094550.2041827933235855No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGCACCATCAATAG1065510.19876552749003112No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATAGA1048040.19550658692142941No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCTGTAGGCACCATCAATAGATCG1026580.19150333193561409No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATAGATCGGA1019960.1902684042559264No Hit
TGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGATCGGAAGAGC1007440.18793286127258962No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATAG954570.17807022888209312No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC947800.17680731945739742No Hit
GTCTAGCGGTTAGGATTCCTCTGTAGGCACCATCAATAGATCGGAAGAGC940730.17548844654268564No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACTGTAGGCACCATCAATAG939550.1752683234819558No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATAG934180.1742665770106684No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGAT931040.173680825815167No Hit
GTCTAGGGGTATGATTCTCTGTAGGCACCATCAATAGATCGGAAGAGCAC907900.16936417528526176No Hit
GGGGGGGGGGATGCGTGCATTTATCAGATCCTGTAGGCACCATCAATAGA890790.16617239090467928No Hit
GCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAG889720.16597278779028868No Hit
TGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGATCGGAAGAGCAC887000.16546538548080977No Hit
GATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCA877650.16372119004197597No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT847580.1581117828926998No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA821650.15327467190564525No Hit
GTATAGTGGTGAGTATCCCCTGTAGGCACCATCAATAGATCGGAAGAGCA802870.14977135743064005No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATA802710.14974151023596483No Hit
GGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGATCGG794540.14821743785786087No Hit
GTCTAGTGGTTAGGATTCGCTGTAGGCACCATCAATAGATCGGAAGAGCA763740.14247185288287897No Hit
CCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATAGATCGGAAG763130.14235806045317967No Hit
TTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAGATCGGAAG756100.14104664933713673No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGC751590.14020533153722867No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCCCCTGTAGGCACCATCAATAGATC743360.13867006646112146No Hit
TTCTGATCGAGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG731420.1364427195584824No Hit
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAG729800.1361405167123957No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCCTGTAGGCACCATCAATAG717030.13375833748737884No Hit
TGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGATC712760.13296179047948362No Hit
TATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT698520.1303053901533881Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCATTGGTGGTTCAGTGGTAGAATTCTGTAGGCACCATCAATAGATCGGA693450.12935960717211673No Hit
CGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAGATCGG692060.12910030966837566No Hit
CCCCCCCGGGCCGCGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACA690040.12872348883560086No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAA672860.125518646307348No Hit
TGATCGAGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT666770.12438258746002202Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAATAGATCGGA627580.1170718902142577No Hit
GGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGA624310.11646188817308269No Hit
GGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGATCGGAAGAG623300.1162734777566953No Hit
TTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAATAGAT617830.11525307678873584No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCTGTAGGCACCATCAATAGATC616080.11492662309697552No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCTGTAGGCACCATCAATAGATCG612230.11420842497510278No Hit
GATGCGTGCATTTATCAGATCCTGTAGGCACCATCAATAGATCGGAAGAG607190.11326823834283302No Hit
ATTTATCAGATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG604520.1127701632816901Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACA604050.1126824871473316No Hit
GCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATAGATCGGA600690.11205569605915178No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGC596890.11134682518561503No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATAGAT583240.10880048638988442No Hit
CCCCCCGCCGGGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT582110.10858969057749061No Hit
TTTCTGATCGAGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC580390.10826883323473188No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCACCATCAAT577870.107798739918597No Hit
TGGGGAAGGTGGCTCGGGGGGGGCCTGTAGGCACCATCAATAGATCGGAA574580.10718500697808758No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT571180.10655075409123892No Hit
GTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAGATCGGAA569880.10630824563450268No Hit
CGTGCATTTATCAGATCTGTAGGCACCATCAATAGATCGGAAGAGCACAC561720.1047860387060659No Hit
TGGGGAAGGTGGCTCGGGGGGCTGTAGGCACCATCAATAGATCGGAAGAG537220.10021568702142122No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)