FastQCFastQC Report
Fri 7 Jul 2023
SRR7513746.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7513746.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62157197
Total Bases3.7 Gbp
Sequences flagged as poor quality0
Sequence length61
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA23577073.79313597426216No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT14348962.308495346081967No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA12274141.974693292556291No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA9238411.4862977170608256No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT5698090.9167224834800708No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG3659180.5886977174984258No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCGG3317530.5337322402102527No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCTGTAGGCACCATCAATAGATCG3021010.48602738633790066No Hit
TCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCAC2885520.46422942784887805No Hit
AGCGGTCGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2819290.4535741854639938No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAATAGATCG2780780.4473786036394144No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAATAGATCGG2751260.4426293547310378No Hit
GCGGTCGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT2672220.42991320860237636Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA2573970.41410651127012693No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACTGTAGGCACCATCAATAG2572980.4139472376786875No Hit
GTATAGTGGTGAGTATCCCCTGTAGGCACCATCAATAGATCGGAAGAGCA2521840.40571971094513803No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAAT2512510.4042186780076328No Hit
GTCTAGGGGTATGATTCTCTGTAGGCACCATCAATAGATCGGAAGAGCAC2503620.4027884333329896No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA2478600.3987631552947924No Hit
AAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATAGATCGGAAGAG2304750.37079374734352966No Hit
TCTGATCGAGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2174650.34986294507456633No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG2092090.33658049284300895No Hit
GTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGGAAG2081210.3348300921613309No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC2045060.3290141928375567No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGATC2042390.32858463678791694No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATAGATCG1931710.31077817102981653No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATAGATC1923770.30950076464998894No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCCTGTAGGCACCATCAATAGAT1901910.3059838750450732No Hit
CCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATAGATCGGAA1710760.27523120130400985No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAGAT1698980.2733360064482959No Hit
ATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGATCGGAAGAGCA1671660.2689406988542292No Hit
TGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGATCGGAAGAGC1655600.26635692725976684No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT1627940.2619069196443977No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCTGTAGGCACCATCAATA1573270.25311147798379646No Hit
TATCAGATCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA1570980.25274305725208296Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAGA1556980.2504907034337472No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCCTGTAGGCACCATCAATAGA1531570.24640268125346773No Hit
GTCTAGTGGTTAGGATTCGCTGTAGGCACCATCAATAGATCGGAAGAGCA1525770.24546956324301433No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGAT1503250.2418464912438056No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCG1483340.2386433223493009No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG1477140.23764585137260935No Hit
TTCTGATCGAGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1472350.23687522460190732No Hit
ATGCGTGCATTTATCAGATCCTGTAGGCACCATCAATAGATCGGAAGAGC1471020.23666125098916543No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA1439690.23162080490856113No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATA1439670.23161758726024922No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATAG1407080.2263744293359947No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCTGTAGGCACCATCAATAGATC1400810.2253656965902114No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCCTGTAGGCACCATCAATAG1367000.2199262621189305No Hit
TGATCGAGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1317790.21200923844748018Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TATCAGATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC1316520.211804917779674Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGCACCATCAATAG1310080.21076883502323956No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG1305300.20999981707669346No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTGTAGGCACCATCAATAGATCGGA1293170.2080483133755211No Hit
ATCCCGGACGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1224880.19706165321451027Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATAGATCGGA1198860.19287549276071764No Hit
GTCTAGCGGTTAGGATTCCTCTGTAGGCACCATCAATAGATCGGAAGAGC1154360.18571622526672174No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAATAGATC1143120.18390790691542927No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG1130670.1819049208412664No Hit
TTTCTGATCGAGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1104560.17770428097007013No Hit
CCCCCCCGGGCCGCGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACA1078670.17353903523030487No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCTGTAGGCACCATCAATAGATCGG1076970.17326553512379267No Hit
GGGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGAT1061700.1708088606376507No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCTGTAGGCACCATCAATAGATCGGA1056710.1700060573838296No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATAGA1054120.16958937192743745No Hit
CCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATAGATCGGAAG1048580.16869808334503886No Hit
GCATTGGTGGTTCAGTGGTAGAATTCCTGTAGGCACCATCAATAGATCGG971180.15624578437795386No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCCTGTAGGCACCATCAAT936270.15062937924951797No Hit
ATCCGAGTCACGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT924600.14875188145951948Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCAC913150.146909777800952No Hit
GGGGGGGGGGATGCGTGCATTTATCAGATCCTGTAGGCACCATCAATAGA909100.14625820401779058No Hit
CGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGATC904520.14552136255436357No Hit
CAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATAGATCGGAAGA895290.14403641785841792No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT890830.14331888228486236No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC882230.1419352935107418No Hit
GGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGATCG868990.13980521032825852No Hit
GCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATAGATCGGA856150.1377394801120134No Hit
GTATAGTGGTGAGTATCCCTGTAGGCACCATCAATAGATCGGAAGAGCAC851560.13700102982443046No Hit
CCCCCCGCCGGGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT820320.13197506316122973No Hit
CGGGGGGGGATGCGTGCATTTATCAGATCCTGTAGGCACCATCAATAGAT818880.13174339248277236No Hit
CGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGATCGGAAG799500.12862549126853323No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAGATCG793730.1276971997305477No Hit
GTATAGTGGTTAGTATCCCCTGTAGGCACCATCAATAGATCGGAAGAGCA788530.12686061116945155No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGC780440.12555907242728465No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCCTGTAGGCACCATCAATAGATC775380.12474500740437186No Hit
TGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATAGATCG751940.12097392358281535No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATAGAT750620.1207615587942294No Hit
ATTTATCAGATCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT741420.11928144057075162Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGGATGCGTGCATTTATCAGATCCTGTAGGCACCATCAATAGATC734640.1181906577930147No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAA729710.11739750748412932No Hit
CTGATCGAGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC724230.11651587184666645No Hit
CCTTCTGATCGAGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA717080.11536556257515924No Hit
TGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGATCGGAAGAGCAC696180.11200312008921509No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATCAATAGATCG693040.11149794930424549No Hit
ATTTATCAGATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG692190.11136119925098939Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCGTGCATTTATCAGATCCTGTAGGCACCATCAATAGATCGGAAGAGCA688540.11077397843406614No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCTGTAGGCACCATCAATAGATCGG686690.1104763459652146No Hit
GCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAG679900.10938395436332175No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATA672850.1082497333333741No Hit
TTGGTGGTTCAGTGGTAGAATTCTCTGTAGGCACCATCAATAGATCGGAA671190.1079826685234857No Hit
GCGGGGCGGACTGTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC650500.10465401134481658No Hit
ATTGGTGGTTCAGTGGTAGAATTCTCTGTAGGCACCATCAATAGATCGGA647500.10417136409803035No Hit
GCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGGCACCAT638720.10275881648910262No Hit
GATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGATCGGAAGAGC637760.10260436937013102No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCCTGTAGGCACCATCAATAGATC637700.10259471642519528No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGG633100.1018546573134564No Hit
TCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC628650.10113873056405681No Hit
TTCCCGGCCAATGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT625060.10056116269206927No Hit
CCCCCCGCCGGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC623380.10029088023386896No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)