FastQCFastQC Report
Fri 7 Jul 2023
SRR7513747.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7513747.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51630482
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length61
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA23646824.5800114746168745No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA16459873.188014010793082No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG14949432.8954658993886597No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAGAT12271892.3768691526064No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG7062061.3678082648928205No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAATAGATCG6926831.3416163730565211No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAGATC6810601.319104477854768No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAATAGATCGG6348451.2295934018202659No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA6086071.1787745851375162No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG5079560.9838296686829303No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGG4217660.8168934002979094No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATAG4000390.7748116703617061No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCGG3953690.7657666260020582No Hit
ATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCAC3718670.7202470044730552No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG3553560.6882678337188485No Hit
TGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACA3518570.6814908293902815No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAGA3478150.6736621207603679No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA3419150.6622347627899349No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC3412170.6608828482368225No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAGA3201610.6201007381647144No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATAGA3195500.6189173287206577No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT3157830.6116212511825864No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA3056660.5920262375237946No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAGATCG3005900.5821948359885541No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGAT2808650.5439906604009624No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGCACCATCAATAG2741000.5308879355416438No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAATAGATC2538030.49157588728302015No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG2467850.4779831418192067No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCCTGTAGGCACCATCAATAGAT2440260.47263939933777877No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCCTGTAGGCACCATCAATAGA2398780.46460538563246423No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATAGA2369300.4588955803279156No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT2348260.45482046826523914No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCG2343270.45385398493858725No Hit
GGGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATC2105280.4077591218303947No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCTGTAGGCACCATCAATA2037180.3945692391560474No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG1939400.3756308143704721No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC1789090.3465181673105434No Hit
TCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAGAT1780660.34488541090900526No Hit
ATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1771420.3430957704404154Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAGATC1662880.3220733054554865No Hit
GATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCA1646430.3188872031061031No Hit
GGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGA1495660.28968546139081175No Hit
CGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAGATCGG1484930.28760723171246005No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTCTGTAGGCACCATCAATAGAT1437280.27837818752108495No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATA1381240.2675241342895075No Hit
AACGATGCCGACTGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGATC1216570.23563018450999548No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCCTGTAGGCACCATCAATAGA1201300.23267262932002067No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCCCCTGTAGGCACCATCAATAGATC1165180.2256767620337149No Hit
CGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAATAGATCGGA1156310.22395878465748198No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATAGATCGGA1149930.22272308052440803No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTACTGTAGGCACCATCAATAG1122220.21735609595897246No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCCTGTAGGCACCATCAAT1026650.19884571288720487No Hit
TTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAATAGAT1017990.1971684091579854No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA995080.19273110795285622No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAA993600.19244445558342843No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATAGATCG968170.18751907061413836No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCTGTAGGCACCATCAATAGATC933080.18072269788223166No Hit
TTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAGATCGGAAG932300.18057162433618187No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATAGAT931360.18038956134478853No Hit
CCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAGA919560.1781040897507019No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCTGTAGGCACCATCAATAGATCGG918590.17791621623830667No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA897990.17392632515032497No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGC889950.1723691055218117No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATAGATC883770.17117213819541718No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATA856740.16593685877269168No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC854860.165572732789905No Hit
GTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAGATCGGAA779410.1509592724700885No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCACCATCAATA778600.1508023884030368No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATAGATCGG772070.14953763166495326No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCTGTAGGCACCATCAATAGATCGGA758470.14690352881075175No Hit
CCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCGGAA744520.14420163654486123No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA729220.14123827083388452No Hit
TTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATAGA717660.13899928340781323No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAAT715650.13860997850068493No Hit
TGGGGGTGGGGGCCGGGCCGCCTGTAGGCACCATCAATAGATCGGAAGAG706080.136756422301074No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT697420.1350791185718545No Hit
TTCGGTTGGCCCCGGATAGCCGGGTCCCCCTGTAGGCACCATCAATAGAT672630.13027769138393866No Hit
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAG668560.12948939736801218No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATA659410.12771718846242808No Hit
GGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGC655320.12692502076583365No Hit
TATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT641840.12431415999563979Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCGGA640580.12407011811355936No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG640440.12404300234888374No Hit
TGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG634250.12284409818215526No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATCA622920.12064965808376532No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCCTGTAGGCACCATCA614080.11893749122853434No Hit
TCTCGGTTGGCCCCGGATAGCCGGGTCCCCCTGTAGGCACCATCAATAGA610810.11830414443932558No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGC608090.11777732386848529No Hit
GCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAG598140.11585016773618345No Hit
TAACGATGCCGACTGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATAG587690.11382616958718302No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGCTGTAGGCACCATCAATAGA584070.11312503338628527No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATAGATCGG582550.11283063365552155No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCCTGTAGGCACCATCAATAG573370.11105261422893553No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGATC566300.10968326811281753No Hit
TGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGG550490.10662112354480828No Hit
GGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAGATCGGA540120.10461262011847962No Hit
ACGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCG534480.1035202421701196No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA531790.1029992321202812No Hit
GCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAATAGATCGGAA528780.1024162431797557No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGG526080.10189329628958335No Hit
CTCGCTTCTGGCGCCAAGCGTCCGCTGTAGGCACCATCAATAGATCGGAA525080.10169961225618618No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA516570.10005136113197625No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)