FastQCFastQC Report
Mon 10 Jul 2023
SRR7513748.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7513748.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53560093
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length61
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA17127573.1978230508300274No Hit
ATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCAC13569332.533477677120538No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG9534621.7801724130688121No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG9175281.7130814167929096No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGG8191721.5294446930852044No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAGAT6829001.2750164567488709No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA6750521.2603637562765249No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA6747981.2598895225966094No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG5832581.0889786916538773No Hit
GATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCA4821850.9002691612204631No Hit
TGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACA4462330.8331445578333855No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAGATC4278970.7989101139163444No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAATAGATCGG4033000.7529859964955625No Hit
TATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT3627990.6773681292898427Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATC3491060.651802452994247No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAATAGATCG3449260.6439981349546947No Hit
GGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGA3379400.6309548416952898No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC3023650.5645341205811574No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCGG2939130.5487537148227133No Hit
ATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA2917600.5447339309138242Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCG2734810.5106059095155044No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA2473590.46183452295349825No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATAGA2400400.44816949813735385No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGC2352850.4392916196019301No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATAGA2329290.43489282216145514No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATAG2309070.43111762333945164No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGAT2180430.40709974121964276No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAGATCG2085540.3893831924451662No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG2071520.38676557189697186No Hit
CCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAG1992160.37194856999221415No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1957680.36551094114044946No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATAGAT1863550.34793628905760116No Hit
GGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGC1706900.31868876702659943No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT1603010.2992918626933676No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCCTGTAGGCACCATCAATAGAT1567540.29266939473013986No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA1561590.29155849299962944No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGC1452310.27115524239287636No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAGA1339650.25012092492072413No Hit
TCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1307010.24402683542763828No Hit
GCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAG1293740.24154924450934018No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTACTGTAGGCACCATCAATAG1289730.24080055275482812No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAGA1210180.22594807667716335No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCCTGTAGGCACCATCAATAGA1178740.22007803459191155No Hit
TGGGGGTGGGGGCCGGGCCGCCTGTAGGCACCATCAATAGATCGGAAGAG1154010.2154607909287984No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG1125160.21007431783212177No Hit
GCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACAC1115370.2082464643965424No Hit
CGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACG1105220.2063513967386128No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGCACCATCAATAG1090020.20351346290604835No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAA1070080.1997905418125394No Hit
ATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGATCGGAAGAGCA1057200.1973857663017874No Hit
TTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1019510.19034881063406667No Hit
TGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1018090.19008368786812974No Hit
AACGATGCCGACTGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGATC1016340.18975695206503843No Hit
GGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAG1008450.18828384035852963No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCCCCTGTAGGCACCATCAATAGATC1001810.18704411136851462No Hit
TTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAGATCGGAAG969230.1809612242458205No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAATAGATC965920.18034322681254494No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATAGATCGGA954350.1781830363886784No Hit
TGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG928850.1734220289722051No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG926420.17296833297134118No Hit
GTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT894530.16701427310815162No Hit
TCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC869590.16235782114866754No Hit
CGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAGATCGG844500.1576733632632042No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA843620.15750906183079255No Hit
TCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAGAT843090.1574101075589992No Hit
TTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA841180.1570534987681967Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACGATGCCGACTGGCGATGCGGCGGCGTCTGTAGGCACCATCAATAGAT818820.1528787487355558No Hit
GCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAA774270.14456098871971712No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC752370.14047212352674593No Hit
TCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC750990.14021446900773676No Hit
GCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT724790.13532276727002696Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGGGCGTGGGGGTGGGGGCCGGGCCGCCTGTAGGCACCATCAATAGATC703930.13142807649717858No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGCTGTAGGCACCATCAATAGA701640.13100051936056198No Hit
CAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAG687830.12842210710873858No Hit
TGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGG681070.1271599733779402No Hit
CATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG680720.12709462621732193Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGATC679150.12680149752540573No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA677470.12648783115443807No Hit
TTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAGATC677370.12646916053711854No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGG670720.1252275644853716No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGTAGGCACCATCAATAGATCGG663640.12390568477915076No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCTGTAGGCACCATCAATA662660.12372271272941965No Hit
CGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAATAGATCGGA661780.12355841129700802No Hit
GGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGA654840.1222626704550345No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCCTGTAGGCACCATCAATAGA617290.11525185365156106No Hit
CGGGCGTGGGGGTGGGGGCCGGGCCGCCTGTAGGCACCATCAATAGATCG611010.11407933888389625No Hit
CCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGA610020.11389449977243318No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATAGAT596580.11138516880469194No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCACCATCAATA587410.10967307319649353No Hit
TTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG587170.1096282637149267No Hit
TTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATAGA585820.10937621038111342No Hit
GTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAGATCGGAA584790.10918390302272253No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT582300.10871900465146692No Hit
AAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATAGATCGGAAGAG578130.10794043990924361No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA567400.10593708267086094No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCTGTAGGCACCATCAATAGATCGG565690.10561781511469744No Hit
TGGGGAAGGTGGCTCGGGGGGGGCTGTAGGCACCATCAATAGATCGGAAG556090.10382543585202512No Hit
GGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGATCG549590.10261184572625742No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCTGTAGGCACCATCAATAGATC542810.10134597787199512No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)