FastQCFastQC Report
Fri 7 Jul 2023
SRR7516858_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7516858_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35040132
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length35-55
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9304072.655261116025476No Hit
GGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAA1800760.5139135891383058No Hit
GCGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAA1767610.5044530083391239No Hit
CCGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAA1383950.39496141167504734No Hit
GGCCTCGCTTCTGGCGCCAAGCGTCCGTCCAAAAAAAAAAAAAA1119500.31949080557116627No Hit
GCGGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAAAAAAAAAAAAAA961330.2743511354352204No Hit
GGCGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAA948850.27078950501670485No Hit
GGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAA923870.2636605364386184No Hit
GGCCCCCGCCCCTTGCCTCTCGGCGCCCCAAAAAAAAAAAAAA893760.2550675322798442No Hit
CCGGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAAAAAAAAAAAAAA777460.22187701804319684No Hit
GGGGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAAAAAAAAAAAAAA731030.20862649718328685No Hit
GCGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAA701410.20017333268036777No Hit
GCGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAA697390.19902607672824976No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG687310.19614937523637183No Hit
GGGCTCGCTTCTGGCGCCAAGCGTCCGTCCAAAAAAAAAAAAAA660360.18845819416433704No Hit
GCGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAA625840.17860663310286617No Hit
GGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAA611790.17459694501150852No Hit
CCGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAA555250.1584611610481376No Hit
GCGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAA550650.15714838060541553No Hit
GGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAA512120.1461524174623543No Hit
CGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAA508310.14506509279131713No Hit
GGCCCCCGCCCCTTGCCTCTCGGCGCCCAAAAAAAAAAAAAA499500.14255083285645156No Hit
CCGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAA496780.14177458007292895No Hit
GGCGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAA466580.13315589107940573No Hit
CCGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAA438990.12528206229360095No Hit
CCCCCCTGTGGTCTAGTGGTTAGGATTCGGCGAAAAAAAAAAAAAAAGAT416790.1189464697222031No Hit
GCGGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAAAAAAAAAAAAAAAGA412150.1176222737973704No Hit
CGCCTCGCTTCTGGCGCCAAGCGTCCGTCCAAAAAAAAAAAAAA407530.11630378561359302No Hit
GCGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAA396930.1132786828542769No Hit
CCGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAA394760.11265939294977541No Hit
CCCCCCTGTGGTCTAGTGGTTAGGATTCGGCGAAAAAAAAAAAAAA393670.11234832106226084No Hit
GCCCTCGCTTCTGGCGCCAAGCGTCCGTCCAAAAAAAAAAAAAA391090.11161202246612542No Hit
GCGGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAA384540.10974273727051029No Hit
GCGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAGA375770.10723989281775537No Hit
GGGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAA371540.10603270558455657No Hit
GGCGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAA365910.10442597647748587No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)