FastQCFastQC Report
Fri 7 Jul 2023
SRR7516859_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7516859_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33228561
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length35-55
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8846692.6623752981659363No Hit
GCGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAA1504940.45290555916640507No Hit
GCGGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAAAAAAAAAAAAAA1295240.3897971988615456No Hit
GGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAA1254370.3774975389394684No Hit
CCGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAA1198640.36072582258377067No Hit
CCGGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAAAAAAAAAAAAAA1043940.3141694881099425No Hit
GGCCTCGCTTCTGGCGCCAAGCGTCCGTCCAAAAAAAAAAAAAA1005790.3026884011016908No Hit
GGCCCCCGCCCCTTGCCTCTCGGCGCCCCAAAAAAAAAAAAAA956490.2878517670386027No Hit
GGGGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAAAAAAAAAAAAAA939890.2828560646968733No Hit
GGCGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAA810630.24395579453470764No Hit
GGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAA720780.2169158032452865No Hit
GCGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAA671210.20199791378266427No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG663810.19977091394358004No Hit
GGCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAA654850.19707443846274292No Hit
GGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAA648800.1952537156213295No Hit
GCGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAA639250.19237968204521408No Hit
GGGCTCGCTTCTGGCGCCAAGCGTCCGTCCAAAAAAAAAAAAAA612340.18428122722497672No Hit
GCGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAA553220.16648930418623906No Hit
CCGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAA510830.1537322064593769No Hit
GCGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAA505410.15210107955020985No Hit
GGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAA486290.14634699347949492No Hit
CGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAA484020.14566384623155965No Hit
CCGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAA452360.13613589827136963No Hit
GGCGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAA442250.13309333497770187No Hit
GGCCCCCGCCCCTTGCCTCTCGGCGCCCAAAAAAAAAAAAAA436750.13143813239459873No Hit
CCGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAA413690.12449831938253361No Hit
CCGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAA408140.12282806950322044No Hit
GCGGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAA405440.1220155155078789No Hit
GGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAA400130.12041749265037387No Hit
GCGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAGA366440.11027862446405669No Hit
GCGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAA360100.10837062730462507No Hit
CGCCTCGCTTCTGGCGCCAAGCGTCCGTCCAAAAAAAAAAAAAA355700.10704646523814257No Hit
GGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAA352560.10610149503615278No Hit
GCGGGGGGGGATGCGTGCATTTATCAGATAAAAAAAAAAAAAA350430.10546048021760557No Hit
GGCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAA347530.10458773703742393No Hit
GCCCTCGCTTCTGGCGCCAAGCGTCCGTCCAAAAAAAAAAAAAA346480.10427174381701333No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)