FastQCFastQC Report
Fri 7 Jul 2023
SRR7516864_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7516864_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38764993
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length35-55
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6875761.7737034029646284No Hit
CGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAA2880870.743162780914213No Hit
CGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAA2379750.613891507732247No Hit
CCGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAA2002480.5165691633170165No Hit
GCGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAA1489700.38429002166980913No Hit
GGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAA1459260.3764375760367092No Hit
CGGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAA1037710.2676925544653136No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG991380.25574104966302974No Hit
CGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAA945040.24378696521369167No Hit
CGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAA907950.23421905428952353No Hit
CCGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAA877890.22646463524448462No Hit
CCGGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAAAAAAAAAAAAAA746080.19246230742257583No Hit
CCCCCCTGTGGTCTAGTGGTTAGGATTCGGCGAAAAAAAAAAAAAA728110.18782668166611047No Hit
CCGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAA710430.183265865674218No Hit
CGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAACATCGGAA686120.1769947436853658No Hit
CGGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAA657460.1696014752279202No Hit
GCGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAA646580.16679481923290945No Hit
CCGGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAAAAAAAAAAAAAA599880.1547478674896188No Hit
CGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAA555150.143209106216013No Hit
CGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAA529080.13648396634561497No Hit
GGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAA529060.1364788070515065No Hit
GCGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAA528290.13628017422833016No Hit
CGGGGGGGGGATGCGTGCATTTATCGAAAAAAAAAAAAAA520600.1342964256436213No Hit
CCGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAA514600.13274863741107862No Hit
GCGGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAAAAAAAAAAAAAA495890.12792211777259963No Hit
CGGGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAAAAAAAAAAAAA492600.1270734138917554No Hit
GGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAA483840.12481364307224303No Hit
CCGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAA453160.11689928590984139No Hit
CCCCCCTGTGGTCTAGTGGTTAGGATTCGAAAAAAAAAAAAAA424660.10954729180526357No Hit
CGGGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAA416150.10735201216210719No Hit
GCGGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAAAAAAAAAAAAAA410370.10586097616475773No Hit
CCGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAACATCGGAA410310.1058454982824323No Hit
CCCCCCCTGTGGTCTAGTGGTTAGGATTCGGCGAAAAAAAAAAAAAA405590.10462790487283204No Hit
CCCCCCTGTGGTCTAGTGGTTAGGATTCGGCAAAAAAAAAAAAAA400890.10341547075734027No Hit
CCGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAA400320.1032684308752487No Hit
CGGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAA397130.10244552346494683No Hit
CCGGGGGGGGATGCGTGCATTTATCGAAAAAAAAAAAAAA394870.10186252323068909No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)