FastQCFastQC Report
Fri 7 Jul 2023
SRR7516866_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7516866_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53731855
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length35-55
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4282510.7970151039825445No Hit
GGGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAA1881310.35012935994858174No Hit
GGGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA1859440.34605914871169063No Hit
GGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAA1496460.27850518095829746No Hit
GGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAA1285060.2391616667617375No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1149810.21399037870551835No Hit
GCGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAA1136720.21155420746222145No Hit
GGCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAA1119970.20843687603936248No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAA1021660.19014046695391404No Hit
CCGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAA872950.16246414719908703No Hit
GGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAA860680.16018058561350618No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAA859810.1600186704888562No Hit
GCGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAA820750.15274923972008783No Hit
CCGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAA805050.14982732310284097No Hit
CCGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA804370.1497007687525398No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAA799010.14870322269722494No Hit
GCGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA759070.1412700157104198No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAAAAAAAAAAAAAA740210.13775999358295No Hit
GGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAA739800.13768368875409195No Hit
GGCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAA633130.1178314055972942No Hit
GGCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA607290.11302234028585093No Hit
GGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAA577230.10742789356518587No Hit
CCCCCCTGTGGTCTAGTGGTTAGGATTCGAAAAAAAAAAAAAA576100.10721759001247955No Hit
GCGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAA561950.10458414287018379No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)