FastQCFastQC Report
Fri 29 Sept 2023
SRR7517655_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7517655_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences227390056
Total Bases11.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAATCTCGTATGCCGTCTT77772793.4202370749229245RNA PCR Primer, Index 3 (95% over 21bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCTCGTATGCCGTCTTC57438552.525992165638061RNA PCR Primer, Index 23 (95% over 22bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGGATCTCGTATGCCGTCTT29922121.3158939544832164RNA PCR Primer, Index 3 (95% over 21bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGCGTATGCCGTCTTC29593101.3014245442641519No Hit
CCCCACTACCACAAATTATGCAGTCGAATCTCGTATGCCGTCTTCTGCTT28766771.2650847845342894RNA PCR Primer, Index 8 (96% over 27bp)
TCAGGATCGGTCGATTGTGCACCTCTATCTCGTATGCCGTCTTCTGCTTG18071320.794727804631879TruSeq Adapter, Index 2 (96% over 27bp)
CAGGATCGGTCGATTGTGCACCTCTATCTCGTATGCCGTCTTCTGCTTGA12690650.5581004826350014RNA PCR Primer, Index 2 (96% over 27bp)
GACCCGACGGCGCGACGACGCCCGGGGCGCACTGATCTCGTATGCCGTCT11409400.501754571009033Illumina PCR Primer Index 5 (95% over 21bp)
TCAGGATCGGTCGATTGTGCACCTCTTATCTCGTATGCCGTCTTCTGCTT10931780.4807501344737784TruSeq Adapter, Index 10 (96% over 27bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGCGTATGCCGTCTTT9573320.4210087357557975No Hit
CAGGATCGGTCGATTGTGCACCTCTTATCTCGTATGCCGTCTTCTGCTTG9508960.4181783569286776RNA PCR Primer, Index 35 (96% over 28bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGCGTATTCCGTCTTC9312860.4095544090107441No Hit
GGGCCTCGATCAGAAGGACTTGGGCCCCCCACGATCTCGTATGCCGTCTT9088680.39969557859645366RNA PCR Primer, Index 1 (95% over 22bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGCGTATTCCGTCTTT7671770.3373837068759067No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGGGGGGGGGG7571400.33296970558818106Illumina Paired End PCR Primer 2 (100% over 24bp)
CGCCGCCGCATCGCCAGTCGGCATCGTTTAATCTCGTATGCCGTCTTCTG7342540.3229050614244978Illumina PCR Primer Index 12 (95% over 24bp)
GCGAGACGGGCCGGTGGTGCGCCCTCGGCGGATCTCGTATGCCGTCTTCT7235960.3182179611231548RNA PCR Primer, Index 30 (95% over 23bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGATCTCGTATGCCGTCTTCT6497090.2857244557783125RNA PCR Primer, Index 17 (95% over 23bp)
CCCCACTACCACAAATTATGCAGTCGGATCTCGTATGCCGTCTTCTGCTT6170470.27136059107175736Illumina PCR Primer Index 9 (96% over 27bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCTCGTATTCCGTCTTC5907410.259791923354819No Hit
ACCCGACGGCGCGACGACGCCCGGGGCGCACTGATCTCGTATGCCGTCTT5590390.24585024069830036Illumina PCR Primer Index 5 (95% over 22bp)
CGCCGCCGCATCGCCAGTCGGCATCGTTATCTCGTATGCCGTCTTCTGCT5499110.24183599303920308RNA PCR Primer, Index 31 (96% over 26bp)
CGCCGCCGCATCGCCAGTCGGCATCGTTTTATCTCGTATGCCGTCTTCTG5319680.2339451466602392RNA PCR Primer, Index 35 (96% over 26bp)
CGCCGCCGCATCGCCAGTCGGCATCGTTAATCTCGTATGCCGTCTTCTGC5116400.22500544175071577RNA PCR Primer, Index 15 (96% over 25bp)
TCGCGTTGGGCTCGACTCCGTGTACATATCTCGTATGCCGTCTTCTGCTT4939960.2172460874894195Illumina PCR Primer Index 10 (96% over 26bp)
GCAGAGTACTAACCACTATACGATCACGATCTCGTATGCCGTCTTCTGCT4642090.20414657006813TruSeq Adapter, Index 1 (96% over 29bp)
CCCCACTACCACAAATTATGCAGTCGAATCTCGTATTCCGTCTTCTGCTT4360910.1917810337317477RNA PCR Primer, Index 46 (96% over 26bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACGGGGGGGGGGGGGGG4123090.1813223529880304Illumina Paired End PCR Primer 2 (100% over 24bp)
CCCCACTACCACAAATTATGCAGTCGATCTCGTATGCCGTCTTCTGCTTG4120730.18121856656739643RNA PCR Primer, Index 23 (96% over 29bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGGG4030110.17723334392423915Illumina Paired End PCR Primer 2 (100% over 24bp)
CCTGGAGGTACTGCAATACCAGGTCAATCTCGTATGCCGTCTTCTGCTTG3987630.17536518835282752RNA PCR Primer, Index 15 (96% over 31bp)
AACGCCGCCGCATCGCCAGTCGGCATCGTTTAATCTCGTATGCCGTCTTC3891210.1711248973877732Illumina PCR Primer Index 12 (95% over 22bp)
TCAGGATCGGTCGATTGTGCACCTCTAATCTCGTATGCCGTCTTCTGCTT3842820.16899683599180784Illumina PCR Primer Index 12 (96% over 27bp)
AACGCCGCCGCATCGCCAGTCGGCATCGTTATCTCGTATGCCGTCTTCTG3746920.1647794132211305RNA PCR Primer, Index 31 (95% over 24bp)
GCCTCTAATACTTGTAATGCTAGAGGTATCTCGTATGCCGTCTTCTGCTT3714250.16334267493209992Illumina PCR Primer Index 2 (96% over 28bp)
CCCCACTACCACAAATTATGCAGTCAATCTCGTATGCCGTCTTCTGCTTG3636660.15993047646727349RNA PCR Primer, Index 15 (96% over 29bp)
GGACCCGACGGCGCGACGACGCCCGGGGCGCACTGATCTCGTATGCCGTC3485420.15327935008732307No Hit
AACGCCGCCGCATCGCCAGTCGGCATCGTTTTATCTCGTATGCCGTCTTC3237310.14236814295872288RNA PCR Primer, Index 35 (95% over 24bp)
CGCCGCGGGGGCGAAACCCGGCGCGCGGAGGGATCTCGTATGCCGTCTTC3212960.141297295779724RNA PCR Primer, Index 17 (95% over 22bp)
AACGCCGCCGCATCGCCAGTCGGCATCGTTAATCTCGTATGCCGTCTTCT3159200.13893307629951945RNA PCR Primer, Index 15 (95% over 23bp)
CCTTCGAGCCGGAAACGAACCAGCGACCTATCTCGTATGCCGTCTTCTGC3052310.13423234303614404Illumina Paired End PCR Primer 2 (96% over 26bp)
GACCCGACGGCGCGACGACGCCCGGGGCGCACTATCTCGTATGCCGTCTT3031980.13333828459059793Illumina PCR Primer Index 1 (95% over 21bp)
GCGAGACGGGCCGGTGGTGCGCCCTCGGCGGATCGCGTATGCCGTCTTCT2807690.12347461667365084RNA PCR Primer, Index 23 (95% over 21bp)
CAGGATCGGTCGATTGTGCACCTCTAATCTCGTATGCCGTCTTCTGCTTG2773530.12197235221227089Illumina PCR Primer Index 12 (96% over 28bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGGGTATTCCGTCTTT2723910.11979019874114459No Hit
ACGCCGCCGCATCGCCAGTCGGCATCGTTTAATCTCGTATGCCGTCTTCT2667880.11732615079702517Illumina PCR Primer Index 12 (95% over 23bp)
CCTGGAGGTACTGCAATACCAGGTCAAATCTCGTATGCCGTCTTCTGCTT2525360.11105850644585794RNA PCR Primer, Index 13 (100% over 28bp)
CCCCGGGACCCGACGGCGCGACGACGCCCGGGGCGCACTGATCTCGTATG2505850.11020050938375246No Hit
CCCCACTACCACAAATTATGCAGTCGCATCTCGTATGCCGTCTTCTGCTT2455040.1079660229293404RNA PCR Primer, Index 24 (96% over 28bp)
CCCCACTACCACAAATTATGCAGTCAAATCTCGTATGCCGTCTTCTGCTT2453390.10789346038949038RNA PCR Primer, Index 13 (96% over 31bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGCGTATTCCGTCTTA2333370.10261530521809625No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)