FastQCFastQC Report
Fri 29 Sept 2023
SRR7517656_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7517656_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287250155
Total Bases14.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCACTACCACAAATTATGCAGTCGAATCTCGTATGCCGTCTTCTGCTT73860012.57127833403606RNA PCR Primer, Index 8 (96% over 27bp)
TCGCGTTGGGCTCGACTCCGTGTACATATCTCGTATGCCGTCTTCTGCTT34849001.213193427171519Illumina PCR Primer Index 10 (96% over 26bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCTCGTATGCCGTCTTC31042451.0806765273982186RNA PCR Primer, Index 23 (95% over 22bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAATCTCGTATGCCGTCTT27724440.9651671032170548RNA PCR Primer, Index 3 (95% over 21bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACGGGGGGGGGGGGGGG23576140.8207529078617903Illumina Paired End PCR Primer 2 (100% over 24bp)
TCGCGTTGGGTTGCTCGACTCCGTGTACATATCTCGTATGCCGTCTTCTG21896630.7622843580362907Illumina PCR Primer Index 6 (95% over 23bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGGGGGGGGGGGGG15984510.556466540461919Illumina Paired End PCR Primer 2 (100% over 24bp)
TCAGGATCGGTCGATTGTGCACCTCTATCTCGTATGCCGTCTTCTGCTTG15878160.5527641925902529TruSeq Adapter, Index 2 (96% over 27bp)
CCCCACTACCACAAATTATGCAGTCGGATCTCGTATGCCGTCTTCTGCTT14387750.5008787549653367Illumina PCR Primer Index 9 (96% over 27bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGGGGGGGGGG12764950.4443844425427725Illumina Paired End PCR Primer 2 (100% over 24bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGGATCTCGTATGCCGTCTT10492010.36525689603196215RNA PCR Primer, Index 3 (95% over 21bp)
CAGGATCGGTCGATTGTGCACCTCTATCTCGTATGCCGTCTTCTGCTTGA10276300.35774741357406753RNA PCR Primer, Index 2 (96% over 27bp)
GCGAGACGGGCCGGTGGTGCGCCCTCGGCGGATCTCGTATGCCGTCTTCT9693270.33745047065335787RNA PCR Primer, Index 30 (95% over 23bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGGG9282180.3231392512216399Illumina Paired End PCR Primer 2 (100% over 24bp)
CCCACTACCACAAATTATGCAGTCGAATCTCGTATGCCGTCTTCTGCTTG8287540.2885129861809822RNA PCR Primer, Index 13 (96% over 31bp)
GACCCGACGGCGCGACGACGCCCGGGGCGCACTGATCTCGTATGCCGTCT8232270.28658887929929927Illumina PCR Primer Index 5 (95% over 21bp)
CCCCACTACCACAAATTATGCAGTCGATCTCGTATGCCGTCTTCTGCTTG6631850.23087367872786702RNA PCR Primer, Index 23 (96% over 29bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGCGTATGCCGTCTTC6399260.22277655515973524No Hit
TCAGGATCGGTCGATTGTGCACCTCTTATCTCGTATGCCGTCTTCTGCTT5917840.20601694714490232TruSeq Adapter, Index 10 (96% over 27bp)
CCCCACTACCACAAATTATGCAGTCGCATCTCGTATGCCGTCTTCTGCTT5730700.19950206815380137RNA PCR Primer, Index 24 (96% over 28bp)
CAGGATCGGTCGATTGTGCACCTCTTATCTCGTATGCCGTCTTCTGCTTG5621030.19568414158035877RNA PCR Primer, Index 35 (96% over 28bp)
CGCCGCCGCATCGCCAGTCGGCATCGTTTAATCTCGTATGCCGTCTTCTG5436340.18925455410111092Illumina PCR Primer Index 12 (95% over 24bp)
GCCTCTAATACTTGTAATGCTAGAGGTATCTCGTATGCCGTCTTCTGCTT4910600.17095204004328562Illumina PCR Primer Index 2 (96% over 28bp)
CGCCGCCGCATCGCCAGTCGGCATCGTTTTATCTCGTATGCCGTCTTCTG4686280.1631428188437357RNA PCR Primer, Index 35 (96% over 26bp)
CCCCACTACCACAAATTATGCAGTCAATCTCGTATGCCGTCTTCTGCTTG4662950.16233063477372187RNA PCR Primer, Index 15 (96% over 29bp)
GACGCCTTATTCGTATCCATCTATATTTATCTCGTATGCCGTCTTCTGCT4645430.16172071343181696Illumina PCR Primer Index 2 (96% over 26bp)
GCGAGACGGGCCGGTGGTGCGCCCTCGGCGGATCTCGTATGCCGTCTTCG4322980.15049530608608375RNA PCR Primer, Index 30 (95% over 22bp)
TGCGATTCGAAAAGTTATTATGAATCATCAAATCTCGTATGCCGTCTTCT4165340.1450074065234186Illumina PCR Primer Index 9 (95% over 24bp)
ACCCGACGGCGCGACGACGCCCGGGGCGCACTGATCTCGTATGCCGTCTT3841240.13372455795541693Illumina PCR Primer Index 5 (95% over 22bp)
GACGCCTTATTCGTATCCATCTATATATCTCGTATGCCGTCTTCTGCTTG3835960.13354074604415792Illumina PCR Primer Index 2 (96% over 28bp)
GACGCCTTATTCGTATCCATCTATATTAATCTCGTATGCCGTCTTCTGCT3647120.12696668518768944Illumina PCR Primer Index 8 (96% over 25bp)
CCCCACTACCACAAATTATGCAGTCAAATCTCGTATGCCGTCTTCTGCTT3176180.1105719159664161RNA PCR Primer, Index 13 (96% over 31bp)
GCAGAGTACTAACCACTATACGATCACGATCTCGTATGCCGTCTTCTGCT2943890.10248523625687861TruSeq Adapter, Index 1 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)