FastQCFastQC Report
Tue 4 Jul 2023
SRR7632989.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7632989.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48651581
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCGAGTGTTACAGCCCCCCCGGCTGGAATTCTCGGGTGCCAAGGAA11943112.4548246438281214RNA PCR Primer, Index 1 (100% over 23bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG11744292.4139585515216866No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGA11051352.2715294699261674RNA PCR Primer, Index 1 (100% over 22bp)
CCCCCCGAGTGTTACAGCCCCCTGGAATTCTCGGGTGCCAAGGAACTCCA9999872.0554049415167004RNA PCR Primer, Index 1 (100% over 28bp)
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCC9219881.8950833273023542No Hit
AGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT7754621.593909147577342RNA PCR Primer, Index 1 (100% over 35bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG6427961.3212232506894277No Hit
CCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA6122831.2585058643829066RNA PCR Primer, Index 1 (100% over 34bp)
CCCCCCGAGTGTTACAGCCCCCCTGGAATTCTCGGGTGCCAAGGAACTCC6053511.2442576121010331RNA PCR Primer, Index 1 (100% over 27bp)
CTCGGATAGCCGGTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA5466511.1236037735341016RNA PCR Primer, Index 1 (100% over 32bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA5058061.0396496672944708No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC4425860.9097052776147193No Hit
TTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATT4220560.867507265591225RNA PCR Primer, Index 27 (100% over 36bp)
CTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA4018700.8260163220595031RNA PCR Primer, Index 1 (100% over 34bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT4000840.8223453211109418No Hit
TCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT3268210.6717582312484357RNA PCR Primer, Index 1 (100% over 35bp)
CAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG2796250.574750078522628RNA PCR Primer, Index 1 (100% over 29bp)
GGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAA2642420.5431313732641083RNA PCR Primer, Index 1 (100% over 23bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA2636810.5419782761016543No Hit
CTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT2564530.527121616047791RNA PCR Primer, Index 1 (100% over 35bp)
GCCTCGGATAGCCGGTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT2546340.5233827858543795RNA PCR Primer, Index 1 (100% over 30bp)
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG2524180.5188279492911032Illumina Small RNA Adapter 2 (100% over 21bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG2477200.5091715313424244Illumina Small RNA Adapter 2 (100% over 21bp)
ACGACCCATTCGAACGTCTGCCCTTGGAATTCTCGGGTGCCAAGGAACTC2413980.49617709237444924RNA PCR Primer, Index 1 (100% over 26bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTG2408650.4950815472985349No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC2386840.490598650843433No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA2186960.4495146827808124No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG2136940.4392334136068466Illumina Small RNA Adapter 2 (100% over 21bp)
GGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATTC2131890.43819542061747185RNA PCR Primer, Index 27 (100% over 37bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG2118370.43541647701027436No Hit
ACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA2002170.4115323610963434RNA PCR Primer, Index 1 (100% over 32bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1928220.3963324439549045RNA PCR Primer, Index 1 (100% over 32bp)
TGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA1913610.3933294582965351No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA1890970.3886759610134766No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA1702220.3498796883908048No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA1629300.3348914807105652No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAGG1618600.3326921688320879Illumina Small RNA Adapter 2 (100% over 21bp)
GTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAAGGAA1609060.3307312870264175RNA PCR Primer, Index 1 (100% over 23bp)
CCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCA1604650.3298248416634189RNA PCR Primer, Index 1 (100% over 28bp)
GGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAAC1592660.32736037910052707RNA PCR Primer, Index 1 (100% over 24bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC1585320.3258516922605249RNA PCR Primer, Index 1 (100% over 24bp)
TCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1572080.32313030073986704RNA PCR Primer, Index 1 (100% over 33bp)
CCCCGCGGGGGCGCGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1497860.3078748869435507RNA PCR Primer, Index 1 (100% over 30bp)
GGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACT1484580.30514527369624433RNA PCR Primer, Index 1 (100% over 25bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG1382470.28415726099425215Illumina Small RNA Adapter 2 (100% over 21bp)
GGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGG1366120.2807966302266724No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGC1350850.2776579860786024No Hit
CGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGGAAC1330950.27356767707096713RNA PCR Primer, Index 1 (100% over 24bp)
AAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1258790.25873568219704923RNA PCR Primer, Index 1 (100% over 30bp)
TGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACT1247100.25633288258402126RNA PCR Primer, Index 1 (100% over 25bp)
GGTTCCATGGTGTAATGGTTAGCACTCTGGACTTGGAATTCTCGGGTGCC1232940.2534223913504476No Hit
GAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATTCCTATCTC1211320.24897854809692618RNA PCR Primer, Index 27 (100% over 44bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGC1181830.24291708012530983No Hit
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG1162660.23897681762900985RNA PCR Primer, Index 1 (100% over 29bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA1152350.23685766758535556No Hit
AGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1116820.22955471888981369RNA PCR Primer, Index 1 (100% over 31bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA1083580.22272246404489918No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG1081720.22234015375574334No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGTGGAATTCTCGGGTGCCAAGGAAC1081380.22227026907923098RNA PCR Primer, Index 1 (100% over 24bp)
CCTCGGATAGCCGGTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1078040.2215837549040801RNA PCR Primer, Index 1 (100% over 31bp)
GCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTC1069790.21988802378282427RNA PCR Primer, Index 1 (100% over 26bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG1031160.2119478912720226Illumina Small RNA Adapter 2 (100% over 21bp)
TCTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGCCAAGGA1015760.208782526512345RNA PCR Primer, Index 1 (100% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCC993020.2041084749126652No Hit
CTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACT990440.20357817354383612RNA PCR Primer, Index 1 (100% over 25bp)
GCAGCTAGGAATAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATT974860.2003758110142402RNA PCR Primer, Index 27 (100% over 36bp)
TGGAGGTGATGAACTGTCTGAGCCTGACCTGGAATTCTCGGGTGCCAAGG925820.190295974143163Illumina Small RNA Adapter 2 (100% over 21bp)
CGACTCTTAGCGGTGGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC919110.18891677949787491RNA PCR Primer, Index 1 (100% over 31bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCG918190.18872767978495908No Hit
AGATAACCTCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATT907870.18660647430964267RNA PCR Primer, Index 27 (100% over 36bp)
CTCGGATAGCCGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT888030.18252849789198014RNA PCR Primer, Index 1 (100% over 35bp)
TTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT879150.18070327457601018RNA PCR Primer, Index 1 (100% over 35bp)
CGGTTGGCCTCGGATAGCCGGTCTGGAATTCTCGGGTGCCAAGGAACTCC859800.1767260143097919RNA PCR Primer, Index 1 (100% over 27bp)
AGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCC849600.17462947401442103RNA PCR Primer, Index 1 (100% over 27bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC823400.16924424305964486RNA PCR Primer, Index 1 (100% over 33bp)
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC822090.16897498151190604No Hit
TCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATTC817960.16812608823544706RNA PCR Primer, Index 27 (100% over 37bp)
TACCTGGTTGATCCTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA810760.16664617743871468RNA PCR Primer, Index 1 (100% over 32bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAG808880.16625975628623454No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC806480.16576645268732376No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA778540.16002357662333727No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCA774630.15921990284344512No Hit
ATTGATCATCGACACTTCGAACGCACTTGGAATTCTCGGGTGCCAAGGAA758410.155885992687473RNA PCR Primer, Index 1 (100% over 23bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC753320.15483977797144968No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA750570.1542745342643644No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATTCCTATCTCGTATGC741910.15249453044496128RNA PCR Primer, Index 27 (100% over 50bp)
CAGGCTGATGAGACTAAGGCGAATGCGACTTGGAATTCTCGGGTGCCAAG741910.15249453044496128No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA738160.15172374357166316No Hit
GTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAA731900.15043704335117083No Hit
CCCCCGCGGGGGCGCGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG729930.15003212331373156RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC729880.15002184615542094No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGACTGGAATTCTCGGGTGCCA719890.14796846992495477No Hit
CTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGG717600.14749777607432737No Hit
TGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAG712880.1465276123298028No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGAATTCTCGGGTGCC712820.14651527973983003No Hit
TCGGATAGCCGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATT704150.1447332204887648RNA PCR Primer, Index 27 (100% over 36bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT700740.14403231829197904RNA PCR Primer, Index 1 (100% over 35bp)
CCGCAGCTAGGAATAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA699280.1437322252693083RNA PCR Primer, Index 1 (100% over 34bp)
GCCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAAC698660.14360478850625635RNA PCR Primer, Index 1 (100% over 24bp)
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCG694290.1427065648699063No Hit
CTCGGATAGCCGGTCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC667060.1371096244539309RNA PCR Primer, Index 1 (100% over 33bp)
ATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC656560.13495142120869616RNA PCR Primer, Index 1 (100% over 31bp)
GGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCA650870.13378188059294518No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG642950.13215397871653956No Hit
GCGAGAATTAATGTGAATTGCAGGACTGGAATTCTCGGGTGCCAAGGAAC638740.13128864198678353RNA PCR Primer, Index 1 (100% over 24bp)
CGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGTGGAATTCTCGGGTGCCA637910.1311180411588269No Hit
ATTGATCATCGACACTTCGAACGCACTGGAATTCTCGGGTGCCAAGGAAC632810.13006977101114145RNA PCR Primer, Index 1 (100% over 24bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA624590.12838020618487198RNA PCR Primer, Index 1 (100% over 34bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC621860.12781907334111095No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGA621390.12772246805299092RNA PCR Primer, Index 1 (100% over 22bp)
AGTAAGGTCAGCTAAATAAGCTATCGGGCTGGAATTCTCGGGTGCCAAGG620330.1275045922968053Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGC611310.12565059293756559No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCA601970.12373081976513774No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA582140.11965489877913732RNA PCR Primer, Index 1 (100% over 23bp)
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTC560710.11525010872719635No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG558690.11483491153144643No Hit
TAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC557520.11459442602697742RNA PCR Primer, Index 1 (100% over 31bp)
GCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATTCCT551540.11336527789302468RNA PCR Primer, Index 27 (100% over 39bp)
GGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATTCC543860.11178670637651016RNA PCR Primer, Index 27 (100% over 38bp)
CGCTGAGAAGACGGTCGAACTTGACTATCTGGAATTCTCGGGTGCCAAGG539520.11089464903514647Illumina Small RNA Adapter 2 (100% over 21bp)
GACCCAGTGGCCTAATGGATAAGGCATCAGCCTTGGAATTCTCGGGTGCC526710.1082616410759601No Hit
GGCCTCGGATAGCCGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA504940.10378696634750677RNA PCR Primer, Index 1 (100% over 32bp)
CAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAGGAACTCCA496270.10200490709644154RNA PCR Primer, Index 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)