FastQCFastQC Report
Tue 4 Jul 2023
SRR7632990.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7632990.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45425707
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCC13554232.983823674995306No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCTGGAATTCTCGGGTGCCAAGGAA12159762.67684551392893RNA PCR Primer, Index 1 (100% over 23bp)
CCCCCCGAGTGTTACAGCCCCCTGGAATTCTCGGGTGCCAAGGAACTCCA11086352.440545394263209RNA PCR Primer, Index 1 (100% over 28bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTC8723341.9203531603811914No Hit
CCCCCCGAGTGTTACAGCCCCCCTGGAATTCTCGGGTGCCAAGGAACTCC8034511.7687143537468772RNA PCR Primer, Index 1 (100% over 27bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG7230041.5916185960517908No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGA6085961.3397612061381896RNA PCR Primer, Index 1 (100% over 22bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCG5712481.2575434434074961No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT5627191.2387677312320091No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG4838181.0650753327845839No Hit
GTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAAGGAA4614671.0158719158735383RNA PCR Primer, Index 1 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC3621100.7971477471996199No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT3616980.796240771772688No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGCC3354630.7384871302058106No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG3122240.6873288730541938No Hit
GGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGG3121980.6872716367408437No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA2777130.6113564726686588No Hit
CTCGGATAGCCGGTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA2588640.5698623468865327RNA PCR Primer, Index 1 (100% over 32bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA2463850.5423911178751714No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG2437520.5365948404501443Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA2391420.5264464018138452No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG2335190.5140679483535612No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA2147260.47269710078480454No Hit
ACGACCCATTCGAACGTCTGCCCTTGGAATTCTCGGGTGCCAAGGAACTC2144840.47216436279131546RNA PCR Primer, Index 1 (100% over 26bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC1998570.4399645337385723No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA1984870.43694862030435766No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCGGGTGCCA1954750.4303180135424199No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGTGGAATTCTCGGGTGCCAAGGAAC1951900.42969061549223664RNA PCR Primer, Index 1 (100% over 24bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA1842160.40553248846517675No Hit
CCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA1758000.3870055341130959RNA PCR Primer, Index 1 (100% over 34bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA1739640.38296376983191477No Hit
CAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG1717840.378164725097179RNA PCR Primer, Index 1 (100% over 29bp)
CTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA1597160.3515982701160821RNA PCR Primer, Index 1 (100% over 34bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG1557490.34286532953686333Illumina Small RNA Adapter 2 (100% over 21bp)
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTC1555140.3423480013200455No Hit
GGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAA1535120.3379408051920909RNA PCR Primer, Index 1 (100% over 23bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG1489910.32798829085918246Illumina Small RNA Adapter 2 (100% over 21bp)
TGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA1396900.30751310045653224No Hit
ATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCC1378420.3034449194153434No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGC1358410.2990399246840561No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC1352150.29766185037032006No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAGG1304110.2870863407805629Illumina Small RNA Adapter 2 (100% over 21bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC1264180.27829616388799405RNA PCR Primer, Index 1 (100% over 24bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC1257740.27687846443424646No Hit
AGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1253410.27592525967730125RNA PCR Primer, Index 1 (100% over 31bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC1222730.26917137470199415No Hit
TCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT1195030.2630735059335455RNA PCR Primer, Index 1 (100% over 35bp)
GGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAAC1181100.2600069603759827RNA PCR Primer, Index 1 (100% over 24bp)
GATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGC1169550.25746434722523964No Hit
CGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGGAAC1166440.25677971286170626RNA PCR Primer, Index 1 (100% over 24bp)
CCCCGCGGGGGCGCGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1147480.2526058647804865RNA PCR Primer, Index 1 (100% over 30bp)
CCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCA1109740.24429779375805863RNA PCR Primer, Index 1 (100% over 28bp)
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGT1090820.2401327512635081No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA1089460.23983336131675395No Hit
TTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATG954710.2101695412247519RNA PCR Primer, Index 15 (100% over 36bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCA948000.20869240406098688No Hit
GCCTCGGATAGCCGGTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT947680.20862195936763298RNA PCR Primer, Index 1 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC928370.20437106240305736No Hit
CTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGG907040.19967548331168514No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG905750.1993915031416022Illumina Small RNA Adapter 2 (100% over 21bp)
TGGAGGTGATGAACTGTCTGAGCCTGACCTGGAATTCTCGGGTGCCAAGG904720.19916475928486924Illumina Small RNA Adapter 2 (100% over 21bp)
ACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA901350.19842288860798576RNA PCR Primer, Index 1 (100% over 32bp)
CTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACT891460.1962457073040162RNA PCR Primer, Index 1 (100% over 25bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGTGGAATTCTC872630.1921004773794715No Hit
TGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAG864540.19031954747561772No Hit
CTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA863460.19008179663554822RNA PCR Primer, Index 1 (100% over 32bp)
GGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCA858470.18898329969856056No Hit
AGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT854240.18805210890828844RNA PCR Primer, Index 1 (100% over 35bp)
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGG853000.187779135721542No Hit
CCCCCGCGGGGGCGCGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG850280.18718035582803366RNA PCR Primer, Index 1 (100% over 29bp)
GTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAA844980.1860136155943594No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGCCAAGGA840780.1850890289940892RNA PCR Primer, Index 1 (100% over 22bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTG838290.184540881223929No Hit
AAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT832900.18335432842024893RNA PCR Primer, Index 1 (100% over 30bp)
CGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGTGGAATTCTCGGGTGCCA831450.18303512590348897No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA823690.1813268420896564No Hit
CTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT820250.18056956163610177RNA PCR Primer, Index 1 (100% over 35bp)
AGTAAGGTCAGCTAAATAAGCTATCGGGCTGGAATTCTCGGGTGCCAAGG808630.17801153870868758Illumina Small RNA Adapter 2 (100% over 21bp)
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCG804050.1770032990350596No Hit
GGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACT800280.17617337249148374RNA PCR Primer, Index 1 (100% over 25bp)
ATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGT788320.173540502077381No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA781060.17194228809691395No Hit
GAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTC760420.16739860537558612RNA PCR Primer, Index 26 (100% over 44bp)
CCTCGGATAGCCGGTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC745380.1640877047879519RNA PCR Primer, Index 1 (100% over 31bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG745170.16404147545793837No Hit
TGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACT743510.16367604361116492RNA PCR Primer, Index 1 (100% over 25bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGC730650.1608450474970043No Hit
CGCGACCTCAGATCAGACGTGGCGACCCTGGAATTCTCGGGTGCCAAGGA729770.160651324590281RNA PCR Primer, Index 1 (100% over 22bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG714190.15722154858261206No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAG697270.15349678542152353No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGTGGAATTCTCG679260.14953207002369825No Hit
CGACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAAGGAA650750.1432558881251975RNA PCR Primer, Index 1 (100% over 23bp)
GGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGA649340.14294549119510677RNA PCR Primer, Index 26 (100% over 37bp)
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCC639390.14075510151113332No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCC632840.1393131866940453No Hit
TCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTG632090.13914808194399703No Hit
TCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGCCAAGGAACTC629710.13862414953717725RNA PCR Primer, Index 1 (100% over 26bp)
TAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCA628050.13825871769040382No Hit
TCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGCCA623830.137329728296799No Hit
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG622110.13695108807002168RNA PCR Primer, Index 1 (100% over 29bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGC618080.13606392521309574No Hit
CCGCAGCTAGGAATAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA615160.1354211173862412RNA PCR Primer, Index 1 (100% over 34bp)
GGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTG585170.12881912878097856No Hit
GCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTC583160.12837664805084928RNA PCR Primer, Index 1 (100% over 26bp)
CGCTGAGAAGACGGTCGAACTTGACTATCTGGAATTCTCGGGTGCCAAGG581770.12807065391409317Illumina Small RNA Adapter 2 (100% over 21bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA578510.12735299860055013RNA PCR Primer, Index 1 (100% over 32bp)
CAGGCTGATGAGACTAAGGCGAATGCGACTTGGAATTCTCGGGTGCCAAG576310.12686869133374193No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCTGGAATTCTCGGG574780.1265318776436435No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC571240.12575258322341576No Hit
AGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCC563170.12397605611289661RNA PCR Primer, Index 1 (100% over 27bp)
TCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC561600.12363043683612893RNA PCR Primer, Index 1 (100% over 33bp)
TGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAAGGA557150.12265081531917599RNA PCR Primer, Index 1 (100% over 22bp)
CGACTCTTAGCGGTGGATCACTCGGTGGAATTCTCGGGTGCCAAGGAACT553170.1217746594455866RNA PCR Primer, Index 1 (100% over 25bp)
TGCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCA544360.11983522898168651No Hit
CATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGC530520.1167884959941295No Hit
CGGTTGGCCTCGGATAGCCGGTCTGGAATTCTCGGGTGCCAAGGAACTCC517150.11384522864993603RNA PCR Primer, Index 1 (100% over 27bp)
GGTTCCATGGTGTAATGGTTAGCACTCTGGACTTGGAATTCTCGGGTGCC517000.11381220769992638No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC514460.11325305294642965No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTCGTATGC509860.11224041047946706RNA PCR Primer, Index 26 (100% over 50bp)
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGATGGAATTCTCGGGTGC491460.10818984061161667No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA485140.10679855791787676RNA PCR Primer, Index 1 (100% over 23bp)
AGCAGAGTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAG483630.10646614702111296No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCT482160.10614254171101838No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG481970.10610071517433951No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGAATTCTCGGGTGCC480500.10577710986424493No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC472800.10408203443041625No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)