FastQCFastQC Report
Tue 4 Jul 2023
SRR7632991.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7632991.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45435039
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCGAGTGTTACAGCCCCCCCGGCTGGAATTCTCGGGTGCCAAGGAA12289872.7049322000141784RNA PCR Primer, Index 1 (100% over 23bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGA10219812.2493234791765007RNA PCR Primer, Index 1 (100% over 22bp)
CCCCCCGAGTGTTACAGCCCCCTGGAATTCTCGGGTGCCAAGGAACTCCA9743072.1443956502381347RNA PCR Primer, Index 1 (100% over 28bp)
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCC7201201.5849441661093324No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG6948791.5293901255372533No Hit
CCCCCCGAGTGTTACAGCCCCCCTGGAATTCTCGGGTGCCAAGGAACTCC5519471.2148047237287505RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG5076601.1173314938719432No Hit
CCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT4969991.0938672243683998RNA PCR Primer, Index 3 (100% over 34bp)
CTCGGATAGCCGGTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA4737601.0427194747208206RNA PCR Primer, Index 1 (100% over 32bp)
TTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAA4240230.9332510972423727RNA PCR Primer, Index 42 (100% over 36bp)
GGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAA3480370.7660101271179717RNA PCR Primer, Index 1 (100% over 23bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC3227860.7104340771007152No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG3112680.6850835981454754Illumina Small RNA Adapter 2 (100% over 21bp)
CTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT2949310.6491267675592839RNA PCR Primer, Index 3 (100% over 34bp)
CGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGGAAC2746120.6044057759034828RNA PCR Primer, Index 1 (100% over 24bp)
TCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA2599300.5720915084941383RNA PCR Primer, Index 10 (100% over 35bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA2589870.5700160178139166RNA PCR Primer, Index 1 (100% over 32bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT2518930.5544025174051243No Hit
ACGACCCATTCGAACGTCTGCCCTTGGAATTCTCGGGTGCCAAGGAACTC2158550.47508487887509027RNA PCR Primer, Index 1 (100% over 26bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG2155990.47452143707855077No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG2150500.47331311853831576No Hit
CCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCA2140800.4711782023561155RNA PCR Primer, Index 1 (100% over 28bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAGG2112560.46496273503803975Illumina Small RNA Adapter 2 (100% over 21bp)
CAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG2071790.45598948423924535RNA PCR Primer, Index 1 (100% over 29bp)
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG2040680.44914234584458046Illumina Small RNA Adapter 2 (100% over 21bp)
GGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAAT1988530.43766442018460683RNA PCR Primer, Index 42 (100% over 37bp)
GGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAAC1966200.4327497110765108RNA PCR Primer, Index 1 (100% over 24bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA1947960.4287351882761672No Hit
CTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA1887710.4154744975568305RNA PCR Primer, Index 10 (100% over 35bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA1818780.4003033869961023No Hit
CGCGACCTCAGATCAGACGTGGCGACCCTGGAATTCTCGGGTGCCAAGGA1775920.39087013879310195RNA PCR Primer, Index 1 (100% over 22bp)
ACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1764240.3882994355963907RNA PCR Primer, Index 1 (100% over 32bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC1713780.3771934695599139No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA1707340.3757760612904943No Hit
AGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA1679150.36957159869500716RNA PCR Primer, Index 10 (100% over 35bp)
CCCCCCGAGTGTTACAGCCCCCCCGGTGGAATTCTCGGGTGCCAAGGAAC1644060.3618484843822848RNA PCR Primer, Index 1 (100% over 24bp)
GCCTCGGATAGCCGGTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1640890.3611507849701637RNA PCR Primer, Index 1 (100% over 30bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG1611050.3545831665292507Illumina Small RNA Adapter 2 (100% over 21bp)
TCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1481500.3260699303020297RNA PCR Primer, Index 1 (100% over 33bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC1457110.3207018266232808RNA PCR Primer, Index 1 (100% over 24bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA1425810.3138128702827789No Hit
CGACTCTTAGCGGTGGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1418680.31224359684163583RNA PCR Primer, Index 1 (100% over 31bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC1406640.3095936596422862No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA1391700.3063054485327943No Hit
CCCCGCGGGGGCGCGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1355940.2984348709373838RNA PCR Primer, Index 1 (100% over 30bp)
GAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGATCTC1294020.28480662248358585RNA PCR Primer, Index 42 (100% over 44bp)
TCTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGCCAAGGA1282130.28218969945200223RNA PCR Primer, Index 1 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT1254060.2760116481907279No Hit
GGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACT1224860.26958489019894977RNA PCR Primer, Index 1 (100% over 25bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGC1212810.26693275205508243No Hit
CTCGGATAGCCGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA1170470.2576139529670042RNA PCR Primer, Index 10 (100% over 35bp)
GCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTC1169950.2574995038520821RNA PCR Primer, Index 1 (100% over 26bp)
CCTCGGATAGCCGGTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1139230.2507382022936087RNA PCR Primer, Index 1 (100% over 31bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA1128370.24834797654735147No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA1060280.23336174532611276No Hit
CGCGACCTCAGATCAGACGTGGCGACCTGGAATTCTCGGGTGCCAAGGAA1059880.23327370754540347RNA PCR Primer, Index 1 (100% over 23bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA1036620.22815431059715827No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG1026130.2258455197980572No Hit
TTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA1018950.22426524163432546RNA PCR Primer, Index 10 (100% over 35bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1018640.22419701235427575RNA PCR Primer, Index 1 (100% over 33bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC1018000.2240561519051409No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGC1017150.22386907162113365No Hit
AGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1012080.22275319275064343RNA PCR Primer, Index 1 (100% over 31bp)
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG1000530.22021110183266268RNA PCR Primer, Index 1 (100% over 29bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGAATTCTCGGGTGCC990460.21799475070330634No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTG984640.21671380099398618No Hit
CTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACT978760.2154196456175596RNA PCR Primer, Index 1 (100% over 25bp)
TACCTGGTTGATCCTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA967670.21297879814739457RNA PCR Primer, Index 1 (100% over 32bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGC962180.21177047960715956No Hit
AAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT953460.20985125598769705RNA PCR Primer, Index 1 (100% over 30bp)
CAGGCTGATGAGACTAAGGCGAATGCGACTTGGAATTCTCGGGTGCCAAG939750.2068337610538862No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCA916720.20176498582954888No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA888700.19559793929086314No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC874060.19237575651690317No Hit
CCCCCGCGGGGGCGCGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG826920.18200050406031346RNA PCR Primer, Index 1 (100% over 29bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA811450.1785956428913817No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA793620.17467135881626514RNA PCR Primer, Index 10 (100% over 35bp)
TGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAG782820.1722943387371143No Hit
GGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGG780770.17184314511097923No Hit
GGCCTCGGATAGCCGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA778870.1714249656526101RNA PCR Primer, Index 1 (100% over 32bp)
ATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC778140.17126429670281565RNA PCR Primer, Index 1 (100% over 31bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA750920.1652733257255485No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG734610.16168358521712725Illumina Small RNA Adapter 2 (100% over 21bp)
GGTTCCATGGTGTAATGGTTAGCACTCTGGACTTGGAATTCTCGGGTGCC730540.16078780079841024No Hit
GCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC728950.1604378506200908RNA PCR Primer, Index 1 (100% over 31bp)
GGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCA726030.159795174820913No Hit
GCCGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGATCT719700.1584019769411885RNA PCR Primer, Index 42 (100% over 43bp)
CCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC717230.15785834364530862RNA PCR Primer, Index 1 (100% over 33bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG714380.15723107445775494Illumina Small RNA Adapter 2 (100% over 21bp)
GTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAA703890.15492228365865385No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCTGGAATTCTCGGGTGCCAAGG695620.1531021025424893Illumina Small RNA Adapter 2 (100% over 21bp)
CGGCGTCCGGTGAGCTCTCGCTGGCTGGAATTCTCGGGTGCCAAGGAACT694750.15291062036944658RNA PCR Primer, Index 1 (100% over 25bp)
TCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAAT691870.1522767483483397RNA PCR Primer, Index 42 (100% over 37bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAG688930.15162967066012645No Hit
TCGGATAGCCGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAA684050.1505556097354731RNA PCR Primer, Index 42 (100% over 36bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGATCTCGTATGC672720.1480619395968825RNA PCR Primer, Index 42 (100% over 50bp)
GTACGACTCTTAGCGGTGGATCACTCGGTGGAATTCTCGGGTGCCAAGGA630020.13866390650616586RNA PCR Primer, Index 1 (100% over 22bp)
CTCGGATAGCCGGTCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC628940.1384262044982508RNA PCR Primer, Index 1 (100% over 33bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA613310.13498612821703532RNA PCR Primer, Index 1 (100% over 23bp)
CGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGTGGAATTCTCGGGTGCCA611440.13457455159221937No Hit
TGGAGGTGATGAACTGTCTGAGCCTGACCTGGAATTCTCGGGTGCCAAGG599730.13199724556195494Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAAC595530.13107284886450743RNA PCR Primer, Index 1 (100% over 24bp)
TCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA581470.1279783208725759RNA PCR Primer, Index 1 (100% over 32bp)
CGGTTGGCCTCGGATAGCCGGTCTGGAATTCTCGGGTGCCAAGGAACTCC578480.12732023846177395RNA PCR Primer, Index 1 (100% over 27bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCC564090.12415307930075728No Hit
GCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCG562660.12383834423472158RNA PCR Primer, Index 42 (100% over 39bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC552420.12158457704856376No Hit
CGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGATCTCGT548440.12070860113050634RNA PCR Primer, Index 42 (100% over 46bp)
CTCGGATAGCCGGTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT548000.12061175957172614RNA PCR Primer, Index 3 (100% over 34bp)
TGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACT547020.12039606700898837RNA PCR Primer, Index 1 (100% over 25bp)
TCGGATAGCCGGTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC534530.11764708730634083RNA PCR Primer, Index 1 (100% over 33bp)
CAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAGGAACTCCA531350.11694718694970198RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC518400.11409696379923874No Hit
CTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT506790.11154166721415162RNA PCR Primer, Index 3 (100% over 34bp)
CGACTCTTAGCGGTGGATCACTCGGTGGAATTCTCGGGTGCCAAGGAACT505730.111308367095272RNA PCR Primer, Index 1 (100% over 25bp)
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCTGGAATTCTCGGG501700.11042138645462592No Hit
CGCGACCTCAGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAA494190.10876847712180901RNA PCR Primer, Index 42 (100% over 36bp)
GGGGATGTAGCTCAGTGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC492600.1084185269434896RNA PCR Primer, Index 1 (100% over 31bp)
CGACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAAGGAA492410.10837670899765267RNA PCR Primer, Index 1 (100% over 23bp)
GTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAAGGAA486610.10710016117736797RNA PCR Primer, Index 1 (100% over 23bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCA476990.10498285255130957No Hit
GGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATC475110.10456907498197592RNA PCR Primer, Index 42 (100% over 38bp)
GACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGG470280.10350601877991124Illumina Small RNA Adapter 2 (100% over 21bp)
CGCTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAG469930.10342898572179061No Hit
ATAGCCGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGA465570.10246937391205936RNA PCR Primer, Index 42 (100% over 40bp)
CGCCGTCGGGCCCGGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA463800.10207980673242077RNA PCR Primer, Index 1 (100% over 32bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG462880.10187731983678938Illumina Small RNA Adapter 2 (100% over 21bp)
CTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGG462190.10172545466506587No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGACTGGAATTCTCGGGTGCCA461830.10164622066242751No Hit
CCTCGGATAGCCGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT459790.10119722798081014RNA PCR Primer, Index 3 (100% over 34bp)
GGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAGGA458070.10081866552376019RNA PCR Primer, Index 1 (100% over 22bp)
AGCAGAGTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAG455980.10035866811955417No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)