FastQCFastQC Report
Tue 4 Jul 2023
SRR7632992.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7632992.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45093655
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGA12531562.779007379197805RNA PCR Primer, Index 1 (100% over 22bp)
CCCCCCGAGTGTTACAGCCCCCCCGGCTGGAATTCTCGGGTGCCAAGGAA11413732.5311166282706514RNA PCR Primer, Index 1 (100% over 23bp)
CCCCCCGAGTGTTACAGCCCCCTGGAATTCTCGGGTGCCAAGGAACTCCA10977232.434318087544689RNA PCR Primer, Index 1 (100% over 28bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG10711452.3753785316359917No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCC9883122.1916875001593903No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG6517931.4454206473172333No Hit
CCCCCCGAGTGTTACAGCCCCCCTGGAATTCTCGGGTGCCAAGGAACTCC6512561.4442297924175806RNA PCR Primer, Index 1 (100% over 27bp)
CTCGGATAGCCGGTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA5070541.1244464437402557RNA PCR Primer, Index 1 (100% over 32bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC4928681.0929874724060402No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT3883870.8612896869858963No Hit
GGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAA3690890.8184943092326403RNA PCR Primer, Index 1 (100% over 23bp)
CCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG3607480.799997250167457RNA PCR Primer, Index 6 (100% over 34bp)
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG2960730.6565735245901003Illumina Small RNA Adapter 2 (100% over 21bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA2858120.6338186602971083No Hit
CAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG2854660.633051368313347RNA PCR Primer, Index 1 (100% over 29bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG2701440.5990731955526781No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA2630370.5833126633891176No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA2625280.5821839014823704No Hit
CTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG2597270.5759723845849266RNA PCR Primer, Index 6 (100% over 34bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG2550890.5656871238315013Illumina Small RNA Adapter 2 (100% over 21bp)
TTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAC2487370.551600884869501RNA PCR Primer, Index 41 (100% over 36bp)
ACGACCCATTCGAACGTCTGCCCTTGGAATTCTCGGGTGCCAAGGAACTC2419020.5364435417798802RNA PCR Primer, Index 1 (100% over 26bp)
GGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAAC2325480.5157000469356499RNA PCR Primer, Index 1 (100% over 24bp)
CGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGGAAC2320950.5146954710147137RNA PCR Primer, Index 1 (100% over 24bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC2299870.5100207556916821No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA2272390.503926771959381No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA2130960.47256315772141333No Hit
GCCTCGGATAGCCGGTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT2121010.4703566388663771RNA PCR Primer, Index 1 (100% over 30bp)
CCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCA2094330.46444006368523466RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG2045240.4535538314647593Illumina Small RNA Adapter 2 (100% over 21bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAGG2040800.45256921400582856Illumina Small RNA Adapter 2 (100% over 21bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC1993490.4420777158116813RNA PCR Primer, Index 1 (100% over 24bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA1915930.4248779567768458No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA1913100.424250374027122No Hit
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1852750.410867116449088RNA PCR Primer, Index 1 (100% over 32bp)
TCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA1758590.38998612997770976RNA PCR Primer, Index 9 (100% over 35bp)
ACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1717320.3808340663448106RNA PCR Primer, Index 1 (100% over 32bp)
GGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACT1706800.3785011438970738RNA PCR Primer, Index 1 (100% over 25bp)
TGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA1525690.3383380655216349No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGTGGAATTCTCGGGTGCCAAGGAAC1448180.32114939452124697RNA PCR Primer, Index 1 (100% over 24bp)
CCTCGGATAGCCGGTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1384730.307078678807473RNA PCR Primer, Index 1 (100% over 31bp)
CTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA1324710.29376860225679197RNA PCR Primer, Index 9 (100% over 35bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGC1286380.2852685150494011No Hit
CTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACT1285620.2850999769258003RNA PCR Primer, Index 1 (100% over 25bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG1271190.2818999701842754No Hit
GCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTC1252650.2777885270111726RNA PCR Primer, Index 1 (100% over 26bp)
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG1248810.2769369659656109RNA PCR Primer, Index 1 (100% over 29bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC1238910.27474153514502203No Hit
AAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1218210.27015108888379086RNA PCR Primer, Index 1 (100% over 30bp)
AGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA1199590.26602190485557226RNA PCR Primer, Index 9 (100% over 35bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGAATTCTCGGGTGCC1181740.2620634765578439No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGC1173650.2602694325842516No Hit
CCCCGCGGGGGCGCGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1167840.2589810029814616RNA PCR Primer, Index 1 (100% over 30bp)
GGTTCCATGGTGTAATGGTTAGCACTCTGGACTTGGAATTCTCGGGTGCC1157380.2566613861750617No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGCCAAGGA1132670.2511816795511475RNA PCR Primer, Index 1 (100% over 22bp)
TCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1110840.24634064371140463RNA PCR Primer, Index 1 (100% over 33bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG1105060.24505886692928308Illumina Small RNA Adapter 2 (100% over 21bp)
CGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGTGGAATTCTCGGGTGCCA1095330.24290113542581546No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCA1094440.2427037684126514No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA1085200.24065469964676847No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGC1079770.23945053910577885No Hit
GAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACGACATCTC1069540.23718192725783704RNA PCR Primer, Index 41 (100% over 44bp)
CAGGCTGATGAGACTAAGGCGAATGCGACTTGGAATTCTCGGGTGCCAAG1062740.23567395457298815No Hit
AGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1043830.23148045994497451RNA PCR Primer, Index 1 (100% over 31bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA1025350.2273823224132087No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC1021620.2265551550434313No Hit
GGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACG1018490.22586104408702287RNA PCR Primer, Index 41 (100% over 37bp)
TGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAG936940.20777645990328353No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA931700.20661443389319406No Hit
GTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAA891860.1977794880454911No Hit
TGGAGGTGATGAACTGTCTGAGCCTGACCTGGAATTCTCGGGTGCCAAGG863320.1914504379829047Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC862780.19133068721087257No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCA806840.17892539427110088No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC794780.17625096036238358No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTG785820.1742639845894062No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAG778100.1725519920707248No Hit
CGACTCTTAGCGGTGGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC776860.17227700881642882RNA PCR Primer, Index 1 (100% over 31bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA765870.16983985884488625RNA PCR Primer, Index 1 (100% over 23bp)
AGTAAGGTCAGCTAAATAAGCTATCGGGCTGGAATTCTCGGGTGCCAAGG761480.16886632942040294Illumina Small RNA Adapter 2 (100% over 21bp)
AGCAGAGTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAG757610.16800811555417275No Hit
ATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC757170.16791054085103546RNA PCR Primer, Index 1 (100% over 31bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG749750.1662650765390386Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA726820.16118010394145252No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGACTGGAATTCTCGGGTGCCA709300.15729485667107712No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC683280.1515246435446406No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTGGAATTCTCGGGTGCCAAGG679220.15062429514751022Illumina Small RNA Adapter 2 (100% over 21bp)
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC676700.15006545821136033No Hit
TGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACT676410.1500011476115653RNA PCR Primer, Index 1 (100% over 25bp)
GACCCAGTGGCCTAATGGATAAGGCATCAGCCTTGGAATTCTCGGGTGCC672770.14919393870379327No Hit
GACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGG632730.1403146407183006Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG626840.13900847026039473No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA615600.13651588011661508No Hit
TTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA592960.13149521811882403RNA PCR Primer, Index 9 (100% over 35bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC592810.13146195401548177RNA PCR Primer, Index 1 (100% over 33bp)
CTCGGATAGCCGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA577850.1281444141088142RNA PCR Primer, Index 9 (100% over 35bp)
CAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAGGAACTCCA567250.1257937508059615RNA PCR Primer, Index 1 (100% over 28bp)
GTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAAGGAA562930.12483574462970456RNA PCR Primer, Index 1 (100% over 23bp)
GTACGACTCTTAGCGGTGGATCACTCGGTGGAATTCTCGGGTGCCAAGGA557850.12370920032984685RNA PCR Primer, Index 1 (100% over 22bp)
CTCGGATAGCCGGTCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC550180.12200829584561286RNA PCR Primer, Index 1 (100% over 33bp)
AGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCC543220.12046484145053223RNA PCR Primer, Index 1 (100% over 27bp)
TGCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCA536530.11898126244146766No Hit
GCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC529480.11741784958438166RNA PCR Primer, Index 1 (100% over 31bp)
CCCCCGCGGGGGCGCGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG527410.11695880495825856RNA PCR Primer, Index 1 (100% over 29bp)
GGCCTCGGATAGCCGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA526770.1168168781173316RNA PCR Primer, Index 1 (100% over 32bp)
CGCGACCTCAGATCAGACGTGGCGACCCTGGAATTCTCGGGTGCCAAGGA521310.1156060647556735RNA PCR Primer, Index 1 (100% over 22bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTC517440.11474785088944331No Hit
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG513650.113907377878329Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCA506430.11230626570412179No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT502710.11148131594123387No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCC502200.1113682179898702No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAG501740.11126620807295395No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCC489620.10857846852289972No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG485040.10756280456751621No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG482130.10691748096267645No Hit
CGGCGTCCGGTGAGCTCTCGCTGGCTGGAATTCTCGGGTGCCAAGGAACT481100.10668906745305963RNA PCR Primer, Index 1 (100% over 25bp)
TACCTGGTTGATCCTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA479730.10638525530920037RNA PCR Primer, Index 1 (100% over 32bp)
GCCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAAC478010.10600382692420919RNA PCR Primer, Index 1 (100% over 24bp)
CGGTTGGCCTCGGATAGCCGGTCTGGAATTCTCGGGTGCCAAGGAACTCC477680.10593064589685622RNA PCR Primer, Index 1 (100% over 27bp)
CGACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAAGGAA474320.1051855299819897RNA PCR Primer, Index 1 (100% over 23bp)
TCGGATAGCCGGTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC464980.10311428514721195RNA PCR Primer, Index 1 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)