FastQCFastQC Report
Tue 4 Jul 2023
SRR7632994.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7632994.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42626464
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGA486574111.4148360980634RNA PCR Primer, Index 1 (100% over 22bp)
GGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAA15422813.618130277003507RNA PCR Primer, Index 1 (100% over 23bp)
TTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGC12666132.971424043054568RNA PCR Primer, Index 11 (100% over 36bp)
CCCCCCGAGTGTTACAGCCCCCCCGGCTGGAATTCTCGGGTGCCAAGGAA11248772.638916988282209RNA PCR Primer, Index 1 (100% over 23bp)
CCCCCCGAGTGTTACAGCCCCCTGGAATTCTCGGGTGCCAAGGAACTCCA8973082.1050491075215625RNA PCR Primer, Index 1 (100% over 28bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG7372371.7295288673252374Illumina Small RNA Adapter 2 (100% over 21bp)
CCCCCCGAGTGTTACAGCCCCCCTGGAATTCTCGGGTGCCAAGGAACTCC6969181.6349420866811755RNA PCR Primer, Index 1 (100% over 27bp)
GGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAAC6550891.5368129057104056RNA PCR Primer, Index 1 (100% over 24bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA6063931.4225740141147996No Hit
CCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG6042691.417591194052596RNA PCR Primer, Index 6 (100% over 34bp)
CCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCA6018021.411803709545319RNA PCR Primer, Index 1 (100% over 28bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA4965631.1649171744576328No Hit
CAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG4730641.1097894491084224RNA PCR Primer, Index 1 (100% over 29bp)
GGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACT4687641.0997018190389896RNA PCR Primer, Index 1 (100% over 25bp)
CTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGG4002780.9390363695191796RNA PCR Primer, Index 11 (100% over 35bp)
TCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC2789780.6544713631419204RNA PCR Primer, Index 1 (100% over 33bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG2597760.6094242299806993No Hit
GAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTC2542660.5964979877289376RNA PCR Primer, Index 11 (100% over 44bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC2521850.5916160439674283No Hit
AAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT2481780.5822157803190056RNA PCR Primer, Index 1 (100% over 30bp)
GTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAAGGAA2423060.5684403003730265RNA PCR Primer, Index 1 (100% over 23bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA2377220.5576864175269147RNA PCR Primer, Index 1 (100% over 32bp)
AGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2284750.5359933209566714RNA PCR Primer, Index 1 (100% over 31bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC2282080.5353669495081741No Hit
CGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGTGGAATTCTCGGGTGCCA2120140.4973764654745934No Hit
TCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCT2033080.4769525335247137RNA PCR Primer, Index 11 (100% over 37bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG2031230.4765185308356799No Hit
GCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTC1873150.439433587547867RNA PCR Primer, Index 1 (100% over 26bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAGG1853260.43476747215063394Illumina Small RNA Adapter 2 (100% over 21bp)
CTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG1817510.426380663430117RNA PCR Primer, Index 6 (100% over 34bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAA1759160.4126919840219447RNA PCR Primer, Index 1 (100% over 23bp)
CCCCCCGAGTGTTACAGCCCCCCCGGTGGAATTCTCGGGTGCCAAGGAAC1639030.3845099607605266RNA PCR Primer, Index 1 (100% over 24bp)
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCC1597100.3746733484625889No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGAATTCTCGGGTGCC1536640.3604896713928699No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG1535380.3601940803722308No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA1527830.3584228802088768No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT1440410.33791449368167153No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA1399360.32828432590608503No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA1382540.3243384203765999No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG1368170.3209672751650242No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG1294270.3036306272084872Illumina Small RNA Adapter 2 (100% over 21bp)
CCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCC1274460.2989832794951042RNA PCR Primer, Index 1 (100% over 27bp)
TCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGG1239290.29073253648250064RNA PCR Primer, Index 11 (100% over 35bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA1173700.27534538168589356No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG1167610.2739166917528041No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC1155630.2711062310962504No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC1145790.2687978059826872RNA PCR Primer, Index 1 (100% over 24bp)
GGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCT1128730.2647955974016517RNA PCR Primer, Index 11 (100% over 37bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC1051170.24660032790897224No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC1045070.24516929201540152No Hit
CGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACAT1029960.2416245457282124RNA PCR Primer, Index 11 (100% over 41bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1025950.24068381557522575RNA PCR Primer, Index 1 (100% over 33bp)
CTCGGATAGCCGGTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1015640.23826513031904312RNA PCR Primer, Index 1 (100% over 32bp)
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG968080.22710774227015407RNA PCR Primer, Index 1 (100% over 29bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC939030.22029272707208367No Hit
ACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA901860.21157279196322734RNA PCR Primer, Index 1 (100% over 32bp)
CCCCGCGGGGGCGCGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT900540.21126312517970058RNA PCR Primer, Index 1 (100% over 30bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGC899140.2109346907123237No Hit
TTTCGTACGTAGCAGAGCAGCTCTGGAATTCTCGGGTGCCAAGGAACTCC887870.20829079324993977RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCTGGAATTCTCGGGTGCCAAGGA874380.20512609256071535RNA PCR Primer, Index 1 (100% over 22bp)
GGTTCCATGGTGTAATGGTTAGCACTCTGGACTTGGAATTCTCGGGTGCC856880.20102066171850427No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC856420.20091274753636615No Hit
CTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACT829210.19452938906684825RNA PCR Primer, Index 1 (100% over 25bp)
ATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACAT824930.19352531798086747RNA PCR Primer, Index 11 (100% over 41bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG813490.19084153919030206Illumina Small RNA Adapter 2 (100% over 21bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGG761110.17855339818944402RNA PCR Primer, Index 11 (100% over 35bp)
GGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTA744900.17475059624931594RNA PCR Primer, Index 11 (100% over 38bp)
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCTGGAATTCTCGGG723180.16965517008401165No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA715050.16774790421274444No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT713680.16742650762681136No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGATGGAATTCTCGGGTGC706900.16583594642051472No Hit
GCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC683260.16029009584280787RNA PCR Primer, Index 1 (100% over 31bp)
GCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTAC640730.15031272591599434RNA PCR Primer, Index 11 (100% over 39bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGC639360.14999132933006126No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC631920.14824593473200123No Hit
TGGAGGTGATGAACTGTCTGAGCCTGACCTGGAATTCTCGGGTGCCAAGG622990.14615099202223295Illumina Small RNA Adapter 2 (100% over 21bp)
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCC611850.14353759204610544No Hit
GGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAAC608180.14267662454948174RNA PCR Primer, Index 1 (100% over 24bp)
CGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCT592310.1389535852657166RNA PCR Primer, Index 11 (100% over 43bp)
CGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGGAAC586530.13759762010754634RNA PCR Primer, Index 1 (100% over 24bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTG583210.13681876122776684No Hit
GACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGG571310.13402706825506333Illumina Small RNA Adapter 2 (100% over 21bp)
CTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGG558770.13108523381155895No Hit
GGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAGGA556140.13046824620498665RNA PCR Primer, Index 1 (100% over 22bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA553860.12993336721525858No Hit
CTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTA542320.12722612881988055RNA PCR Primer, Index 11 (100% over 38bp)
GTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA540040.12669124983015245RNA PCR Primer, Index 1 (100% over 32bp)
CCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACA538950.12643554013769476RNA PCR Primer, Index 11 (100% over 40bp)
GACCCAGTGGCCTAATGGATAAGGCATCAGCCTTGGAATTCTCGGGTGCC528190.12391128665985526No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA527840.12382917804301102RNA PCR Primer, Index 1 (100% over 23bp)
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATGGAATTCTCGGGTG524920.1231441575824821No Hit
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATC513940.1205682929740548RNA PCR Primer, Index 11 (100% over 42bp)
TGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAG509500.11952668652037382No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCG502030.11777425404087001No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGC500390.11738951652194281RNA PCR Primer, Index 11 (100% over 50bp)
CGCGACCTCAGATCAGACGTGGCGACCTGGAATTCTCGGGTGCCAAGGAA452960.10626262595931017RNA PCR Primer, Index 1 (100% over 23bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG452390.10612890621187815No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)