FastQCFastQC Report
Tue 4 Jul 2023
SRR7632995.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7632995.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44958365
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCC16109213.583139644869203No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCTGGAATTCTCGGGTGCCAAGGAA10823152.40737179832941RNA PCR Primer, Index 1 (100% over 23bp)
CCCCCCGAGTGTTACAGCCCCCTGGAATTCTCGGGTGCCAAGGAACTCCA9795892.178880393003616RNA PCR Primer, Index 1 (100% over 28bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG9178472.041548886397448No Hit
CCCCCCGAGTGTTACAGCCCCCCTGGAATTCTCGGGTGCCAAGGAACTCC6865651.5271129188083241RNA PCR Primer, Index 1 (100% over 27bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT6230381.3858110720885868No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG6079271.3521999743540496No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC4983951.1085701181526508No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG4809111.0696808035612504Illumina Small RNA Adapter 2 (100% over 21bp)
GGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGG4735871.0533901755546493No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA4429420.9852271095712666No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGA3536640.7866478240478718RNA PCR Primer, Index 1 (100% over 22bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA3429850.7628947360519005No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA3234090.7193522273330002No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG3157040.702214148579469No Hit
ACGACCCATTCGAACGTCTGCCCTTGGAATTCTCGGGTGCCAAGGAACTC3016550.6709652363914924RNA PCR Primer, Index 1 (100% over 26bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA2942800.6545611700959321No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA2765000.6150134685725337No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA2722020.6054535123775076No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT2699470.6004377605813734No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC2282170.5076185488506978No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC2105830.4683955922329471No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC2066640.45967863822449945No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC2064230.45914258670216324No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA2018830.44904435470462506No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCA1999100.44465584991802976No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG1977930.43994704878613805No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA1970020.4381876431671837No Hit
TGGAGGTGATGAACTGTCTGAGCCTGACCTGGAATTCTCGGGTGCCAAGG1909890.4248130464708848Illumina Small RNA Adapter 2 (100% over 21bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAGG1909220.42466401969911494Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG1858850.41346032045426917Illumina Small RNA Adapter 2 (100% over 21bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG1853750.4123259375646779Illumina Small RNA Adapter 2 (100% over 21bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA1779470.3958039844198071No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGC1695680.3771667408278749No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGTGGAATTCTCGGGTGCCAAGGAAC1666130.3705939929087724RNA PCR Primer, Index 1 (100% over 24bp)
TCCCTGGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAAC1598960.3556535029688024RNA PCR Primer, Index 1 (100% over 24bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG1578130.3510203273628834No Hit
CGACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAAGGAA1545670.3438003139126612RNA PCR Primer, Index 1 (100% over 23bp)
CCCCCGCGGGGGCGCGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG1510840.3360531460608054RNA PCR Primer, Index 1 (100% over 29bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG1415870.314929157232475Illumina Small RNA Adapter 2 (100% over 21bp)
TCTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGCCAAGGA1408580.31330765698441215RNA PCR Primer, Index 1 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGC1327600.29529543612184295No Hit
CAGGCTGATGAGACTAAGGCGAATGCGACTTGGAATTCTCGGGTGCCAAG1202030.2673651499559648No Hit
GGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAA1158100.25759388714425RNA PCR Primer, Index 1 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCTGGAATTCTCGGGTGCCAAGGAA1154360.25676200635854973RNA PCR Primer, Index 1 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGA1153550.2565818396643205RNA PCR Primer, Index 1 (100% over 22bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC1143350.25431307388513796RNA PCR Primer, Index 1 (100% over 24bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAG1137510.2530140942625471No Hit
CGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGGAAC1137110.25292512305552034RNA PCR Primer, Index 1 (100% over 24bp)
TGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAG1122740.24972883244308372No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCA1068300.2376198511667406No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC1065870.237079351084053No Hit
CTCGGATAGCCGGTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1063600.23657443948417609RNA PCR Primer, Index 1 (100% over 32bp)
AGTAAGGTCAGCTAAATAAGCTATCGGGCTGGAATTCTCGGGTGCCAAGG1029120.2289051214384687Illumina Small RNA Adapter 2 (100% over 21bp)
GGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCA1025800.22816666042014652No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCC948550.21098409606310195No Hit
GTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAA922650.2052232104081187No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTGGAATTCTCGGGTGCCAAGGAACT896180.19933554078312232RNA PCR Primer, Index 1 (100% over 25bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG879350.19559207724747107No Hit
TGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACT868040.19307641636878922RNA PCR Primer, Index 1 (100% over 25bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA850120.18909050629399002No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGC849510.1889548252032742No Hit
GGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAAC834130.18553388229309495RNA PCR Primer, Index 1 (100% over 24bp)
GGTTCCATGGTGTAATGGTTAGCACTCTGGACTTGGAATTCTCGGGTGCC827520.18406363309697762No Hit
CGACTCTTAGCGGTGGATCACTCGGTGGAATTCTCGGGTGCCAAGGAACT812350.18068940007048745RNA PCR Primer, Index 1 (100% over 25bp)
CCCCGCGGGGGCGCGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT803760.1787787433995876RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAACT800320.1780135910191574RNA PCR Primer, Index 1 (100% over 25bp)
CGCGACCTCAGATCAGACGTGGCGACCCTGGAATTCTCGGGTGCCAAGGA796810.17723286867749752RNA PCR Primer, Index 1 (100% over 22bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTG788390.17536002476958404No Hit
AGCAGAGTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAG761490.1693767110970339No Hit
CAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG736690.16386049626137428RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC729840.1623368643410409No Hit
CGACTCTTAGCGGTGGATCACTCGGCTGGAATTCTCGGGTGCCAAGGAAC726390.161569487680435RNA PCR Primer, Index 1 (100% over 24bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCG711470.15825086165833654No Hit
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGG695880.15478320886446825No Hit
GTACGACTCTTAGCGGTGGATCACTCGGTGGAATTCTCGGGTGCCAAGGA659540.1467001747060864RNA PCR Primer, Index 1 (100% over 22bp)
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA653010.1452477197513744No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGAA646260.1437463306327977RNA PCR Primer, Index 1 (100% over 23bp)
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCC643730.1431835877483534No Hit
GATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCTGGAATTCTCGGGTGC618710.13761843874882906No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGAATTCTCGGGTGCC618490.13756950458496434No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCTGGAATTCTCGGGTGCCAAGGAAC610750.13584791172899638RNA PCR Primer, Index 1 (100% over 24bp)
CTCGGTTGGCCTCGGATAGCCGGTCCCCTGGAATTCTCGGGTGCCAAGGA608400.1353252058877141RNA PCR Primer, Index 1 (100% over 22bp)
TAGGATGGGTGTGATAGGTGGCACGGAGATGGAATTCTCGGGTGCCAAGG604040.13435541973112233Illumina Small RNA Adapter 2 (100% over 21bp)
GCCTCGGATAGCCGGTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT600810.1336369772343812RNA PCR Primer, Index 1 (100% over 30bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCC594830.132306857689331No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC582170.1294909189869338No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCCTGGAATTCTCGGGTGCCAAGGAA579170.12882363493423304RNA PCR Primer, Index 1 (100% over 23bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCTGGAATTCTCGGGT560720.12471983801012336No Hit
CCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCA552550.12290260110660164RNA PCR Primer, Index 1 (100% over 28bp)
CTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACT547640.12181047954034806RNA PCR Primer, Index 1 (100% over 25bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA544410.12109203704360691No Hit
GCCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAAC542930.12076284357760785RNA PCR Primer, Index 1 (100% over 24bp)
TAGGATGGGGTGTGATAGGTGGCACGGAGATGGAATTCTCGGGTGCCAAG541700.12048925711600056No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAG535730.11916136185112604No Hit
GGAGGTGATGAACTGTCTGAGCCTGACCTGGAATTCTCGGGTGCCAAGGA533130.11858304900545205RNA PCR Primer, Index 1 (100% over 22bp)
GAGAATTAATGTGAATTGCAGGACTGGAATTCTCGGGTGCCAAGGAACTC531940.11831835966454742RNA PCR Primer, Index 1 (100% over 26bp)
CGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGTGGAATTCTCGGGTGCCA515500.11466164305574725No Hit
GTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAAGGAA510120.11346498032123722RNA PCR Primer, Index 1 (100% over 23bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA505820.11250853984569947RNA PCR Primer, Index 1 (100% over 23bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA505450.1124262414791997No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC497590.11067795726112371No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGCC489500.10887851459900733No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCTGGAATTCTCGGG484840.10784200003714549No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTC477650.10624274259083934No Hit
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG472230.10503718273562662Illumina Small RNA Adapter 2 (100% over 21bp)
GCAAAGTGATGAGTAATACTGGCTGGAGCCCTGGAATTCTCGGGTGCCAA466430.10374710023373847No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATTCTCGGGTGCCAAGG461170.10257712886133648Illumina Small RNA Adapter 2 (100% over 21bp)
CAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAGGAACTCCA460620.10245479345167467RNA PCR Primer, Index 1 (100% over 28bp)
GCGAGAATTAATGTGAATTGCAGGACTGGAATTCTCGGGTGCCAAGGAAC457290.10171410815317683RNA PCR Primer, Index 1 (100% over 24bp)
CGACTCTTAGCGGTGGATCACTCGGCTCTGGAATTCTCGGGTGCCAAGGA456700.10158287562281236RNA PCR Primer, Index 1 (100% over 22bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATTGGAATTCTCGG450550.1002149433147758No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)