FastQCFastQC Report
Tue 4 Jul 2023
SRR7632996.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7632996.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42599307
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGA42372849.94683786757376RNA PCR Primer, Index 1 (100% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA10372732.434952756391084No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG10015892.3511861354927674No Hit
GGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAA9593552.2520436776119386RNA PCR Primer, Index 1 (100% over 23bp)
CCCCCCGAGTGTTACAGCCCCCCCGGCTGGAATTCTCGGGTGCCAAGGAA7148711.6781282380955165RNA PCR Primer, Index 1 (100% over 23bp)
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCC6151901.4441314737819562No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG5759341.3519797399521076Illumina Small RNA Adapter 2 (100% over 21bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTG4689931.1009404448762512No Hit
CCCCCCGAGTGTTACAGCCCCCTGGAATTCTCGGGTGCCAAGGAACTCCA4664761.0950318980541163RNA PCR Primer, Index 1 (100% over 28bp)
TTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGT4242410.9958870927172594RNA PCR Primer, Index 13 (100% over 36bp)
GGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACT4193400.9843822107246957RNA PCR Primer, Index 1 (100% over 25bp)
CAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG3998460.9386209029175052RNA PCR Primer, Index 1 (100% over 29bp)
CTCGGATAGCCGGTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA3883710.9116838449977602RNA PCR Primer, Index 1 (100% over 32bp)
GGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAAC3758540.8823007378969804RNA PCR Primer, Index 1 (100% over 24bp)
GTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAAGGAA3649450.8566923400890066RNA PCR Primer, Index 1 (100% over 23bp)
CCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA3580320.8404643765683794RNA PCR Primer, Index 1 (100% over 34bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA3565770.8370488280478365No Hit
CCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCA3249420.7627870566063434RNA PCR Primer, Index 1 (100% over 28bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAGG3150200.7394955978978719Illumina Small RNA Adapter 2 (100% over 21bp)
CCCCCCGAGTGTTACAGCCCCCCTGGAATTCTCGGGTGCCAAGGAACTCC2927680.6872600063658313RNA PCR Primer, Index 1 (100% over 27bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT2844980.6678465450153919No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC2815270.6608722531566066RNA PCR Primer, Index 1 (100% over 24bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG2461510.5778286487148723No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG2326710.546184941459259Illumina Small RNA Adapter 2 (100% over 21bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC2316260.5437318499101406No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG2249570.5280766656603123No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCG2242470.5264099718805285No Hit
CGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGTGGAATTCTCGGGTGCCA2017930.4737001942308592No Hit
AAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1989580.4670451563918634RNA PCR Primer, Index 1 (100% over 30bp)
AGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1933390.4538548009712928RNA PCR Primer, Index 1 (100% over 31bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGC1893490.44448845142011345No Hit
CTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAG1762840.4138189384160639RNA PCR Primer, Index 13 (100% over 35bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA1737620.40789865431378963No Hit
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1477330.34679672136450485RNA PCR Primer, Index 1 (100% over 32bp)
TCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1416740.3325734852916739RNA PCR Primer, Index 1 (100% over 33bp)
ACGACCCATTCGAACGTCTGCCCTTGGAATTCTCGGGTGCCAAGGAACTC1381700.32434799937003667RNA PCR Primer, Index 1 (100% over 26bp)
CTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA1351920.3173572753190562RNA PCR Primer, Index 1 (100% over 34bp)
CTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACT1345850.31593236951014253RNA PCR Primer, Index 1 (100% over 25bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG1331730.31261776159879784No Hit
CTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGG1331000.3124463973087637No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA1315280.30875619643296076No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA1272220.29864805077697626No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA1235680.2900704464511594No Hit
CGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGGAAC1199150.28149518958137043RNA PCR Primer, Index 1 (100% over 24bp)
TGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA1198830.28142007098847877No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCG1107100.2598868568448778No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA1106340.2597084501867601No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCC1091130.2561379695683782No Hit
GCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTC1076050.2525980058783585RNA PCR Primer, Index 1 (100% over 26bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA1068000.2507083037759276No Hit
ATTGATCATCGACACTTCGAACGCACTGGAATTCTCGGGTGCCAAGGAAC1066420.25033740572352503RNA PCR Primer, Index 1 (100% over 24bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC1059150.24863080519126757No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAA1045190.2453537565763687RNA PCR Primer, Index 1 (100% over 23bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG1007650.23654140664776543No Hit
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG977550.22947556400389332RNA PCR Primer, Index 1 (100% over 29bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG949000.2227735770443402Illumina Small RNA Adapter 2 (100% over 21bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC939370.22051297688950666No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGTGGAATTCTCGGGTGCCAAGGAAC915800.2149800230318301RNA PCR Primer, Index 1 (100% over 24bp)
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTC898070.21081798349442635No Hit
GACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGG890090.20894471358419045Illumina Small RNA Adapter 2 (100% over 21bp)
GGTTCCATGGTGTAATGGTTAGCACTCTGGACTTGGAATTCTCGGGTGCC887670.20837662922544728No Hit
GCCTCGGATAGCCGGTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT886320.20805972266168554RNA PCR Primer, Index 1 (100% over 30bp)
TGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAG870680.2043883014341055No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC833340.19562290062605947No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGG831220.19512523994815223No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA813740.19102188681144508RNA PCR Primer, Index 1 (100% over 23bp)
TGGAGGTGATGAACTGTCTGAGCCTGACCTGGAATTCTCGGGTGCCAAGG810110.19016976027333027Illumina Small RNA Adapter 2 (100% over 21bp)
GACCCAGTGGCCTAATGGATAAGGCATCAGCCTTGGAATTCTCGGGTGCC809730.19008055694427142No Hit
CGGTTGGCCTCGGATAGCCGGTCTGGAATTCTCGGGTGCCAAGGAACTCC808140.18970731143584096RNA PCR Primer, Index 1 (100% over 27bp)
ATTGATCATCGACACTTCGAACGCACTTGGAATTCTCGGGTGCCAAGGAA805210.18901950681967666RNA PCR Primer, Index 1 (100% over 23bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGAATTCTCGGGTGCC782290.18363913760381126No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG779320.1829419431635355Illumina Small RNA Adapter 2 (100% over 21bp)
GTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAA778470.182742409401167No Hit
ATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCC762120.17890431879560859No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCTGGAATTCTCGGG758620.178082709185856No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGC756670.17762495526042243No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC743160.1744535421667775No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCC711070.16692055577336035No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC701670.16471394710716772No Hit
ATTGATCATCGACACTTCGAACGCATGGAATTCTCGGGTGCCAAGGAACT689820.1619322117141483RNA PCR Primer, Index 1 (100% over 25bp)
AGTAAGGTCAGCTAAATAAGCTATCGGGCTGGAATTCTCGGGTGCCAAGG689520.1618617880333124Illumina Small RNA Adapter 2 (100% over 21bp)
GGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGG688910.16171859321561263No Hit
GAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC670160.15731711316336672RNA PCR Primer, Index 13 (100% over 44bp)
GGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCA647610.1520235998205323No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCTGGAATTCTCGGGT642430.1508076175980985No Hit
CCTCGGATAGCCGGTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC635060.14907754250556235RNA PCR Primer, Index 1 (100% over 31bp)
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGATGGAATTCTCGGGTGC629630.14780287388243193No Hit
TCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTC623570.14638031552954606RNA PCR Primer, Index 13 (100% over 37bp)
GGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAGGA598470.14048820089960617RNA PCR Primer, Index 1 (100% over 22bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC575750.13515478080429807RNA PCR Primer, Index 1 (100% over 33bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA566020.1328707060891859No Hit
GATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGC563300.1322321980496068No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA562150.13196224060640235No Hit
TTTCGTACGTAGCAGAGCAGCTCTGGAATTCTCGGGTGCCAAGGAACTCC561990.13192468130995652RNA PCR Primer, Index 1 (100% over 27bp)
CAGGCTGATGAGACTAAGGCGAATGCGACTTGGAATTCTCGGGTGCCAAG558480.13110072424417607No Hit
GCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC555360.13036831796348236RNA PCR Primer, Index 1 (100% over 31bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG542560.1273635742478158Illumina Small RNA Adapter 2 (100% over 21bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG539630.1266757696316515Illumina Small RNA Adapter 2 (100% over 21bp)
TCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAG523190.1228165519218423RNA PCR Primer, Index 13 (100% over 35bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAG511050.11996674030401482No Hit
GGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAGGAAC503280.11814276697036409RNA PCR Primer, Index 1 (100% over 24bp)
ACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA495180.11624132758779385RNA PCR Primer, Index 1 (100% over 32bp)
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCGGGTGCCA493250.11578826857441601No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAG492420.11559342972410325No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGCCAAGGA489890.11499952334905356RNA PCR Primer, Index 1 (100% over 22bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGTGGAATTCTCGG476690.1119008813922724No Hit
ATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC463100.10871068865040458RNA PCR Primer, Index 1 (100% over 31bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCA458800.10770128255842285No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGC457010.10728108792943508No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAA454730.106745867955082RNA PCR Primer, Index 1 (100% over 23bp)
CGCTGAGAAGACGGTCGAACTTGACTATCTGGAATTCTCGGGTGCCAAGG449180.10544302985961719Illumina Small RNA Adapter 2 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCA448700.1053303519702797No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATGGAATTCTCGGGTG448500.10528340284972242No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGACTGGAATTCTCGGGTGCCA445690.1046237677058925No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGTGGAATTCTCGGG436070.10236551500708685No Hit
TAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCA430290.10100868542298118No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)