FastQCFastQC Report
Fri 7 Jul 2023
SRR7632997.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7632997.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48813435
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTC16529393.3862378257133514No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGA10688782.189720924167701RNA PCR Primer, Index 1 (100% over 22bp)
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGCC10256392.1011408027318708No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCG9616481.9700477952432565No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCTGGAATTCTCGGGTGCCAAGGAA8953881.834306477304865RNA PCR Primer, Index 1 (100% over 23bp)
GTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAAGGAA7505361.5375603048627904RNA PCR Primer, Index 1 (100% over 23bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG6277571.2860332406436876No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCC5721881.1721936798752228No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCGGGTGCCA4859200.9954636464325857No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG4799910.983317400219837No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC3438200.7043552661270406No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA3099420.6349522421439917No Hit
GGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAA3050840.6250000640192602RNA PCR Primer, Index 1 (100% over 23bp)
ACGACCCATTCGAACGTCTGCCCTTGGAATTCTCGGGTGCCAAGGAACTC2976510.6097726988481756RNA PCR Primer, Index 1 (100% over 26bp)
CCCCCCGAGTGTTACAGCCCCCTGGAATTCTCGGGTGCCAAGGAACTCCA2740490.5614212562586509RNA PCR Primer, Index 1 (100% over 28bp)
ATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCC2651210.5431312096761886No Hit
CGCGACCTCAGATCAGACGTGGCGACCCTGGAATTCTCGGGTGCCAAGGA2530990.5185027441727876RNA PCR Primer, Index 1 (100% over 22bp)
GATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGC2510260.5142559625234323No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG2488570.5098125137065236Illumina Small RNA Adapter 2 (100% over 21bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG2461170.5041993049659381No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC2389750.4895680871464997No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA2388140.4892382599175821No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT2312250.47369131059922337No Hit
CCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT2283790.4678609485277977RNA PCR Primer, Index 3 (100% over 34bp)
CCCCCCGAGTGTTACAGCCCCCCTGGAATTCTCGGGTGCCAAGGAACTCC2262810.46356295147022536RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT2077940.42569018140190296No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAGG2067360.42352274532615863Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG2035560.41700814540095366Illumina Small RNA Adapter 2 (100% over 21bp)
AGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2034700.4168319643966871RNA PCR Primer, Index 1 (100% over 31bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA1926820.3947314914428784No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG1809440.37068483297682286No Hit
GGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAAC1795360.36780038118604025RNA PCR Primer, Index 1 (100% over 24bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA1757380.3600197363697105No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA1753060.3591347341157204No Hit
CCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCA1749980.35850376028648673RNA PCR Primer, Index 1 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC1749280.358360357143479No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG1729770.35436350668622274Illumina Small RNA Adapter 2 (100% over 21bp)
CTCGGATAGCCGGTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1719630.35228620972894037RNA PCR Primer, Index 1 (100% over 32bp)
TTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAT1632440.3344243239591723RNA PCR Primer, Index 44 (100% over 36bp)
TCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGCCA1623350.3325621317164014No Hit
GGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGG1606580.3291266021332037No Hit
CTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1594440.3266395819101852RNA PCR Primer, Index 1 (100% over 32bp)
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTC1532400.3139299662070493No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA1527790.3129855540795275No Hit
CAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG1525350.31248569169532936RNA PCR Primer, Index 1 (100% over 29bp)
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGG1511710.30969137902300875No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG1443780.2957751283022799No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC1411960.2892564311444175No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT1378190.28233825380246236No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGC1358330.27826970177370225No Hit
TCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGCCAAGGAACTC1343370.2752049717459958RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCTGGAATTCTCGGGTGCCAAGGAA1331740.27282243095574RNA PCR Primer, Index 1 (100% over 23bp)
GGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTG1308330.26802662013029815No Hit
CGACTCTTAGCGGTGGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1276300.26146490202953343RNA PCR Primer, Index 1 (100% over 31bp)
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGT1255810.2572672871720665No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCTGGAATTCTCGGGTGCCAAGG1249610.25599714504828436Illumina Small RNA Adapter 2 (100% over 21bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC1248030.25567346366835275No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC1237610.25353880545386737RNA PCR Primer, Index 1 (100% over 24bp)
CGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGGAAC1218520.2496279968824157RNA PCR Primer, Index 1 (100% over 24bp)
CCCCCCGAGTGTTACAGCCCCCCCGGTGGAATTCTCGGGTGCCAAGGAAC1216020.24911584280024546RNA PCR Primer, Index 1 (100% over 24bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGAATTCTCGGGTGCC1204340.24672305892834626No Hit
ATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGT1191170.2440250312234736No Hit
GAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTATAATATCTC1178670.2414642608126226RNA PCR Primer, Index 44 (100% over 44bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA1135900.2327023287748547No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC1134930.23250361299097266No Hit
ATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGAACTCC1122620.22998176629036657RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC1107480.22688016116874382No Hit
GGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACT1103230.22600949922905447RNA PCR Primer, Index 1 (100% over 25bp)
TCTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGCCAAGGA1101470.2256489427552066RNA PCR Primer, Index 1 (100% over 22bp)
CTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGG1075000.22022625533318851No Hit
TCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTG1058340.21681326052960626No Hit
CATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGC1056000.2163338843086949No Hit
CTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA1052690.21565579230390158RNA PCR Primer, Index 10 (100% over 35bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGC1007160.20632844215941779No Hit
TGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAAGGA976390.2000248497160669RNA PCR Primer, Index 1 (100% over 22bp)
AAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT967230.19814831715899528RNA PCR Primer, Index 1 (100% over 30bp)
GCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTC959190.19650122963073588RNA PCR Primer, Index 1 (100% over 26bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTC953110.19525567090289794No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCA950250.19466976663289523No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA948860.1943850089632086No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGACTTGGAATTCTCGGGTGCC946410.1938830979626818No Hit
GACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGCCAAG932070.1909453821473535No Hit
TAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCA917740.18800971494835386No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGTGGAATTCTC893520.18304796620028893No Hit
CTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACT883690.18103417634919566RNA PCR Primer, Index 1 (100% over 25bp)
CTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT867430.17770312619876064RNA PCR Primer, Index 3 (100% over 34bp)
CGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGTGGAATTCTCGGGTGCCA859260.17602940665822842No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA857380.17564426678843642No Hit
CAGGCTGATGAGACTAAGGCGAATGCGACTTGGAATTCTCGGGTGCCAAG848310.17378617177832292No Hit
AGCAGAGTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAG834880.17103488004890458No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA812250.16639886129709988No Hit
TTGAAAAAATTAGAGTGTTCAAAGCAGGCCCTGGAATTCTCGGGTGCCAA811830.16631281941129528No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA795370.16294079693428665No Hit
AGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA794900.16284451196683863RNA PCR Primer, Index 10 (100% over 35bp)
TCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA789040.16164402279823167RNA PCR Primer, Index 10 (100% over 35bp)
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCG777730.15932703773049367No Hit
TCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC770930.15793397862699068RNA PCR Primer, Index 1 (100% over 33bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGTGGAATTCTCG752690.15419730244347687No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA732840.15013079903104545No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTATAATATCTCGTATGC730080.14956538092432956RNA PCR Primer, Index 44 (100% over 50bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC726050.14873978854387115No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCTGGAATTCTCGGG721640.14783634874292292No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGGAATTCTCG714290.1463306157413425No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC711460.14575085732032583No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAG709450.145339085438261No Hit
TCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCGGGTGCCAA703830.14418776306154238No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTTGGAATTCTCGG690180.14139140177289305No Hit
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA675450.1383737899207462RNA PCR Primer, Index 1 (100% over 32bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTTGGAATTCTC660960.1354053448604877No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG659040.13501201052538098Illumina Small RNA Adapter 2 (100% over 21bp)
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA655000.13418436952859392No Hit
ACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA654750.1341331541203769RNA PCR Primer, Index 1 (100% over 32bp)
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG654220.13402457745495683RNA PCR Primer, Index 1 (100% over 29bp)
TCGAACGCACTTGCGGCCCCGGGTGGAATTCTCGGGTGCCAAGGAACTCC644280.1319882528242481RNA PCR Primer, Index 1 (100% over 27bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC641600.1314392236481616RNA PCR Primer, Index 1 (100% over 33bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGC619650.1269425108067072No Hit
GGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTATA618620.12673150332485308RNA PCR Primer, Index 44 (100% over 37bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG617450.12649181521439742Illumina Small RNA Adapter 2 (100% over 21bp)
TGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACT610180.12500247114344648RNA PCR Primer, Index 1 (100% over 25bp)
GCCTCGGATAGCCGGTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT606470.12424243448550588RNA PCR Primer, Index 1 (100% over 30bp)
GTGGTAATTCTAGAGCTAATACATGCCGTGGAATTCTCGGGTGCCAAGGA599150.12274284733291153RNA PCR Primer, Index 1 (100% over 22bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATTGGAATTCTCGG597750.12245604104689622No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG591760.12122891986601639No Hit
ATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCGGGTG582270.1192847829700983No Hit
TGGAGGTGATGAACTGTCTGAGCCTGACCTGGAATTCTCGGGTGCCAAGG580470.11891603203093574Illumina Small RNA Adapter 2 (100% over 21bp)
CGCTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAG569470.11666255406938685No Hit
TACCTGGTTGATCCTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA564620.11566897514997664RNA PCR Primer, Index 1 (100% over 32bp)
GTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAA562070.11514657798616303No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCTGGAATTCTCGGGT561590.11504824440238634No Hit
TGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAG555220.11374327580101666No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG551940.11307132964520936No Hit
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG546980.11205521594618367Illumina Small RNA Adapter 2 (100% over 21bp)
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCTGGAATTCTCGGG546080.1118708404766024No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCAA545660.1117847985907978No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA542840.11120708878610981RNA PCR Primer, Index 1 (100% over 23bp)
GGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCA540680.11076458765911475No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAA539930.11061094143446368No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCT530150.10860739466501385No Hit
CGACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAAGGAA514510.10540335872695704RNA PCR Primer, Index 1 (100% over 23bp)
CCCCGCGGGGGCGCGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT513960.10529068482887959RNA PCR Primer, Index 1 (100% over 30bp)
TCATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCGGGTGC502750.1029941859244284No Hit
CGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGCCAA499140.10225463542977462No Hit
ACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAAG495170.10144133474728832No Hit
CTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAGGA489120.10020192186843643RNA PCR Primer, Index 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)