FastQCFastQC Report
Tue 4 Jul 2023
SRR7632998.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7632998.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47320115
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGA10862332.2954994931859316RNA PCR Primer, Index 1 (100% over 22bp)
CCCCCCGAGTGTTACAGCCCCCCCGGCTGGAATTCTCGGGTGCCAAGGAA10635222.2475051043303678RNA PCR Primer, Index 1 (100% over 23bp)
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCC6969871.4729190746894847No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG6918781.4621223976315358No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG6781851.4331854434419695No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT6506851.375070622715097No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC6460201.3652122358536112No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG5236901.106696380598399No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC4995971.0557814578430336No Hit
CCCCCCGAGTGTTACAGCCCCCTGGAATTCTCGGGTGCCAAGGAACTCCA4918001.039304321217309RNA PCR Primer, Index 1 (100% over 28bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCG4904511.0364535251023799No Hit
CCCCCCGAGTGTTACAGCCCCCCTGGAATTCTCGGGTGCCAAGGAACTCC3898330.8238209057606897RNA PCR Primer, Index 1 (100% over 27bp)
TTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAC3787520.8004038029070725RNA PCR Primer, Index 43 (100% over 36bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA3748310.7921176861045244No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT3663400.7741739427302744No Hit
CCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT3533320.7466845758933596RNA PCR Primer, Index 3 (100% over 34bp)
GGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAA3038160.6420440863256567RNA PCR Primer, Index 1 (100% over 23bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTC3018870.6379675958099426No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG2887370.6101781451714561Illumina Small RNA Adapter 2 (100% over 21bp)
GTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAAGGAA2789830.5895653465761864RNA PCR Primer, Index 1 (100% over 23bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA2723380.5755226926223658No Hit
CTCGGATAGCCGGTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA2606900.5509073678286708RNA PCR Primer, Index 1 (100% over 32bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG2594860.5483629953139378No Hit
GGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACA2544490.537718473422983RNA PCR Primer, Index 43 (100% over 37bp)
CGCGACCTCAGATCAGACGTGGCGACCCTGGAATTCTCGGGTGCCAAGGA2448000.5173275677795796RNA PCR Primer, Index 1 (100% over 22bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG2236910.4727186313896321Illumina Small RNA Adapter 2 (100% over 21bp)
CTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT2174420.45951283085427835RNA PCR Primer, Index 3 (100% over 34bp)
CCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCA2158900.45623304169907447RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA2144080.4531011811784481No Hit
GGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAAC2130690.4502715177256015RNA PCR Primer, Index 1 (100% over 24bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA2125600.44919586522560223No Hit
ACGACCCATTCGAACGTCTGCCCTTGGAATTCTCGGGTGCCAAGGAACTC2089910.4416536181283583RNA PCR Primer, Index 1 (100% over 26bp)
TCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA2082690.4401278399260019RNA PCR Primer, Index 10 (100% over 35bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA1952780.4126743986146272No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAGG1911520.40395506223938804Illumina Small RNA Adapter 2 (100% over 21bp)
CAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG1851230.3912141802698493RNA PCR Primer, Index 1 (100% over 29bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC1783350.3768693292482489No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC1778880.37592469925316113No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA1771020.3742636720134767No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG1726760.3649103557757626Illumina Small RNA Adapter 2 (100% over 21bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA1717770.3630105294545459No Hit
GGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGG1712650.36192853715592194No Hit
CTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA1672460.35343532026496555RNA PCR Primer, Index 10 (100% over 35bp)
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCGGGTGCCA1622560.3428901218857984No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGC1613260.34092478431212603No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC1595670.33720754905181444No Hit
GAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTC1520610.32134537289269055RNA PCR Primer, Index 43 (100% over 44bp)
CCCCCCGAGTGTTACAGCCCCCCCGGTGGAATTCTCGGGTGCCAAGGAAC1501170.3172371833838527RNA PCR Primer, Index 1 (100% over 24bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG1496140.316174210481103No Hit
CGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGGAAC1483710.3135474205842484RNA PCR Primer, Index 1 (100% over 24bp)
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGCC1452880.3070322208642139No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC1374810.29053395157640677No Hit
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1314410.2777698236785773RNA PCR Primer, Index 1 (100% over 32bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGAATTCTCGGGTGCC1287780.27214219576600773No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGTGGAATTCTC1261320.26655049337897846No Hit
ACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1221060.2580424836245643RNA PCR Primer, Index 1 (100% over 32bp)
AGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1209540.2556080009526604RNA PCR Primer, Index 1 (100% over 31bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA1202080.254031504361306No Hit
CGCGACCTCAGATCAGACGTGGCGACCTGGAATTCTCGGGTGCCAAGGAA1186910.25082567952339085RNA PCR Primer, Index 1 (100% over 23bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA1176500.24862576940060266No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGC1167170.24665409202830552No Hit
CAGGCTGATGAGACTAAGGCGAATGCGACTTGGAATTCTCGGGTGCCAAG1127890.23835318236229983No Hit
TCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1117290.23611312018155495RNA PCR Primer, Index 1 (100% over 33bp)
AGTAAGGTCAGCTAAATAAGCTATCGGGCTGGAATTCTCGGGTGCCAAGG1108890.234337976566625Illumina Small RNA Adapter 2 (100% over 21bp)
AGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA1100510.23256705948411158RNA PCR Primer, Index 10 (100% over 35bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCA1091410.23064398723460414No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC1075820.22734940521594252No Hit
GGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACT1053090.22254595112459044RNA PCR Primer, Index 1 (100% over 25bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC1045770.22099904026015152No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA1041280.22005018373264731No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCAA1032670.21823066152734413No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGCCAAGGA1027950.21723319987705017RNA PCR Primer, Index 1 (100% over 22bp)
CTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1009790.2133955084428683RNA PCR Primer, Index 1 (100% over 32bp)
AGCAGAGTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAG1000740.21148300252440216No Hit
GATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGC990290.209274639336781No Hit
ATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCC983810.20790524283383505No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGACTTGGAATTCTCGGGTGCC974770.2059948501815771No Hit
GCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTC966890.2043295964094762RNA PCR Primer, Index 1 (100% over 26bp)
GCCTCGGATAGCCGGTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT943070.19929579630142488RNA PCR Primer, Index 1 (100% over 30bp)
CTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGG914780.19331736619828585No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAATGGAATTCTCG902370.19069480283384774No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTC898110.18979455142913323No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG890520.18819058237707156Illumina Small RNA Adapter 2 (100% over 21bp)
AAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT881880.18636472037314364RNA PCR Primer, Index 1 (100% over 30bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAG854080.1804898403142089No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC838190.17713186030929975No Hit
CGACTCTTAGCGGTGGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC823320.17398943345763213RNA PCR Primer, Index 1 (100% over 31bp)
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGG806230.170377861507733No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA797180.16846535558926684No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGC793530.16769401342325563No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA791890.16734743776510264No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC784160.16571388298612547RNA PCR Primer, Index 1 (100% over 24bp)
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCTGGAATTCTCGGG746270.15770671732306651No Hit
GCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGC739300.15623377077591633RNA PCR Primer, Index 43 (100% over 39bp)
TCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACA737760.15590832777984584RNA PCR Primer, Index 43 (100% over 37bp)
CTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACT725700.15335972873269643RNA PCR Primer, Index 1 (100% over 25bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC724500.15310613678770646RNA PCR Primer, Index 1 (100% over 33bp)
CCCCGCGGGGGCGCGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT721350.15244045793210773RNA PCR Primer, Index 1 (100% over 30bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG720770.15231788849202924Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA706380.1492768984183576No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTCTCGGGTGCCAAGG702360.14842736540264112Illumina Small RNA Adapter 2 (100% over 21bp)
GGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCA683400.14442061267179931No Hit
TACCTGGTTGATCCTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA679580.14361334498024783RNA PCR Primer, Index 1 (100% over 32bp)
TTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA677020.14307234883093586RNA PCR Primer, Index 10 (100% over 35bp)
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGT660260.139530514665909No Hit
TGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAG657530.13895359299105678No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATTCTCGGGTGCCAAG655660.13855841221011403No Hit
TGGAGGTGATGAACTGTCTGAGCCTGACCTGGAATTCTCGGGTGCCAAGG641410.13554700786335788Illumina Small RNA Adapter 2 (100% over 21bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTCGTATGC639700.13518563934174715RNA PCR Primer, Index 43 (100% over 50bp)
GGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAG639050.1350482770382109RNA PCR Primer, Index 43 (100% over 38bp)
GTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAA619660.13095065386041432No Hit
TGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAAGGA619600.13093797426316484RNA PCR Primer, Index 1 (100% over 22bp)
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG610340.1289810897543254RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG606050.1280744985509862No Hit
CATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGC605390.1279350229812417No Hit
CGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGTGGAATTCTCGGGTGCCA600600.12692276846748998No Hit
CCTCGGATAGCCGGTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC566320.11967849190560928RNA PCR Primer, Index 1 (100% over 31bp)
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCG564630.11932134991641503No Hit
AGCAGAGTCGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA556920.11769202166985436No Hit
GGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTG550010.11623175471995364No Hit
CTCGGATAGCCGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA546030.1153906747690702RNA PCR Primer, Index 10 (100% over 35bp)
GTACGACTCTTAGCGGTGGATCACTCGGTGGAATTCTCGGGTGCCAAGGA536410.11335771267673377RNA PCR Primer, Index 1 (100% over 22bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG535640.11319499117869852Illumina Small RNA Adapter 2 (100% over 21bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCTGGAATTCTCGGGT533340.11270893995080104No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCC528770.11174317729363084No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTG525430.11103734638007537No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA525130.11097394839382788RNA PCR Primer, Index 10 (100% over 35bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG509020.10756947653233724No Hit
ATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGT501370.10595282788302607No Hit
CCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC481740.10180448631623147RNA PCR Primer, Index 1 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)