FastQCFastQC Report
Tue 4 Jul 2023
SRR7632999.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7632999.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45916734
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG32003276.96984894439574No Hit
CCCCCCGAGTGTTACAGCCCCCTGGAATTCTCGGGTGCCAAGGAACTCCA21570774.697801459485337RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC15324473.3374477374632088No Hit
CCCCCCGAGTGTTACAGCCCCCCTGGAATTCTCGGGTGCCAAGGAACTCC10355632.2553063116379315RNA PCR Primer, Index 1 (100% over 27bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT9454952.0591512453825658No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG9038431.9684392186953017No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC6843461.4904065258648405No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCTGGAATTCTCGGGTGCCAAGGAA6594231.436127839580228RNA PCR Primer, Index 1 (100% over 23bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA6346721.3822237443978485No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA5575771.2143219942428831No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT5444201.1856679527773033No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA4643641.0113175732402917No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG4640081.0105422567728795Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG4477970.975237045387418No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA3498580.761940080494401No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC3488220.7596838224600209No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA3462290.7540366438083336No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA3358430.7314174392281472No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA3285990.7156410558294498No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG3117960.6790465541386284No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA3111810.6777071731626209No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA3081610.6711300503210877No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCC2994900.6522458674870037No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCA2729280.5943976764549499No Hit
AGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA2695890.5871258177900893RNA PCR Primer, Index 10 (100% over 35bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG2672900.5821189285805911Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG2517840.548349105143236Illumina Small RNA Adapter 2 (100% over 21bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC2149980.46823452208077343No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCC2149810.4681974985415992No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCTGGAATTCTCGGG2035330.4432654116906486No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC1888910.41137725518544066No Hit
CGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGGAAC1853790.40372862756310146RNA PCR Primer, Index 1 (100% over 24bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGA1762390.3838230306188589RNA PCR Primer, Index 1 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC1636770.3564648130243758No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC1578200.34370911485124356No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATGGAATTCTCGGGTG1563060.3404118420094948No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGAATTCTCGGGTGCC1497650.3261664908484127No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGATGGAATTCTCGGGTGC1482690.3229084194010837No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTGGAATTCTCGGGTGCCAAGG1479270.3221635929071087Illumina Small RNA Adapter 2 (100% over 21bp)
TGCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCA1422670.30983693221734804No Hit
CAGGCTGATGAGACTAAGGCGAATGCGACTTGGAATTCTCGGGTGCCAAG1275460.27777672514774243No Hit
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCTGGAATTCTCGGGT1246970.2715720155531968No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGC1201760.261725931988107No Hit
CGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGTGGAATTCTCGGGTGCCA1181850.25738982219423534No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA1127890.24563811528929735No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA1123360.2446515468630674No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAG1072090.23348568301917988No Hit
GCCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAAC1068640.23273432295946836RNA PCR Primer, Index 1 (100% over 24bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG1063930.23170855313881863Illumina Small RNA Adapter 2 (100% over 21bp)
CGACTCTTAGCGGTGGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1034680.22533832654561187RNA PCR Primer, Index 1 (100% over 31bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGC1023520.2229078400915884No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA990620.21574269633375928No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG978980.21320767282794983No Hit
CCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT961180.20933109049088727RNA PCR Primer, Index 3 (100% over 34bp)
CCCCGCGGGGGCGCGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT956570.208327099222693RNA PCR Primer, Index 1 (100% over 30bp)
TGGAGGTGATGAACTGTCTGAGCCTGACCTGGAATTCTCGGGTGCCAAGG944110.2056134915867492Illumina Small RNA Adapter 2 (100% over 21bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG939160.20453545324020647No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA898330.19564327027266357No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTG886570.1930821125039076No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATTGGAATTCTCGGGT863730.1881078911230925No Hit
TACCTGGTTGATCCTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA862500.18784001492789099RNA PCR Primer, Index 1 (100% over 32bp)
AGTAAGGTCAGCTAAATAAGCTATCGGGCTGGAATTCTCGGGTGCCAAGG784280.17080483119727113Illumina Small RNA Adapter 2 (100% over 21bp)
AGCAGAGTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAG774570.16869013375385103No Hit
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGATGGAATTCTCGGGTGCC769420.16756853830239754No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGTGGAATTCTCGGGTGCCAAGGAAC752090.1637943151618754RNA PCR Primer, Index 1 (100% over 24bp)
GTTTCCGTAGTGTAGTGGTTATCACTTTCGCCTTGGAATTCTCGGGTGCC713260.15533770324344062No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAG710500.15473661519567136No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGTGGAATTCTCGGG702430.15297908601251997No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA684000.14896529879498832No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCTGGAATTCTCGGGT680040.1481028681177542No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG679380.1479591296715485No Hit
GGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAA670160.14595114713515991RNA PCR Primer, Index 1 (100% over 23bp)
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCCA665840.14501031366908632No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATTCTCGGGTGCCAAGGA656510.14297837472499678RNA PCR Primer, Index 1 (100% over 22bp)
CTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT635210.13833954305199495RNA PCR Primer, Index 3 (100% over 34bp)
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATGGAATTCTCGGGTGC630190.13724625971873347No Hit
TTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAG614650.13386187266716312RNA PCR Primer, Index 10 (100% over 36bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGC606420.1320694978000831No Hit
GCGAGAATTAATGTGAATTGCAGGACTGGAATTCTCGGGTGCCAAGGAAC593950.12935371230889375RNA PCR Primer, Index 1 (100% over 24bp)
GGGGATGTAGCTCAGTGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC576730.12560344557607253RNA PCR Primer, Index 1 (100% over 31bp)
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCTGGAATTCTCGGGT569930.12412250400910482No Hit
GAGAATTAATGTGAATTGCAGGACTGGAATTCTCGGGTGCCAAGGAACTC548350.11942269238922786RNA PCR Primer, Index 1 (100% over 26bp)
GCATGTTGGAACAATGTAGGTAAGGGAAGTCGGCTGGAATTCTCGGGTGC548090.11936606815284379No Hit
CATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGC546190.11895227565619104No Hit
ATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCGGGTG543040.11826625125384571No Hit
TAGGATGGGTGTGATAGGTGGCACGGAGATGGAATTCTCGGGTGCCAAGG532320.11593159043062601Illumina Small RNA Adapter 2 (100% over 21bp)
GACCCAGTGGCCTAATGGATAAGGCATCAGCCTTGGAATTCTCGGGTGCC531980.11585754335227763No Hit
CGAGAATTAATGTGAATTGCAGGACTGGAATTCTCGGGTGCCAAGGAACT529280.11526952243598161RNA PCR Primer, Index 1 (100% over 25bp)
GGTTCCATGGTGTAATGGTTAGCACTCTGGACTTGGAATTCTCGGGTGCC514400.11202887383061695No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC514180.11198096101521507No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC505220.11002960271521053No Hit
TCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA503510.10965718946822306RNA PCR Primer, Index 10 (100% over 35bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG498390.1085421275825062Illumina Small RNA Adapter 2 (100% over 21bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG497970.1084506576621935No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC495350.10788005958786179No Hit
CCCCCGCGGGGGCGCGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG495340.10787788173261625RNA PCR Primer, Index 1 (100% over 29bp)
AACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCTGGAATTCTCGGG491960.1071417666596235No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGCC489410.10658641357201058No Hit
CGCGACCTCAGATCAGACGTGGCGACCCTGGAATTCTCGGGTGCCAAGGA483870.105379881765981RNA PCR Primer, Index 1 (100% over 22bp)
TGGATATGATGACTGATTACCTGAGATGGAATTCTCGGGTGCCAAGGAAC476570.10379004743673624RNA PCR Primer, Index 1 (100% over 24bp)
TAGGATGGGGTGTGATAGGTGGCACGGAGATGGAATTCTCGGGTGCCAAG473900.10320856008617686No Hit
ATTGATCATCGACACTTCGAACGCACTGGAATTCTCGGGTGCCAAGGAAC471300.10264231772233626RNA PCR Primer, Index 1 (100% over 24bp)
CTCGGATAGCCGGTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA470720.10251600211809489RNA PCR Primer, Index 1 (100% over 32bp)
GGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAAC469590.1022699044753488RNA PCR Primer, Index 1 (100% over 24bp)
GGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGC468580.10204994109554917RNA PCR Primer, Index 10 (100% over 37bp)
GGGGATGTAGCTCAGTGGTAGAGTGGAATTCTCGGGTGCCAAGGAACTCC463510.10094576848606002RNA PCR Primer, Index 1 (100% over 27bp)
CTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA463200.10087825497344825RNA PCR Primer, Index 10 (100% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)