FastQCFastQC Report
Tue 4 Jul 2023
SRR7633002.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7633002.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40966829
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG25624826.255016711203106No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCG23059075.628717321518831No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTC20238214.940145599260318No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT10685152.6082443432465814No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC10037652.450189640013387No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG8573142.0927028547901525No Hit
CCCCCCGAGTGTTACAGCCCCCTGGAATTCTCGGGTGCCAAGGAACTCCA6639141.6206135944766435RNA PCR Primer, Index 1 (100% over 28bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA6205511.5147645427963194No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT5970841.4574816127457657No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCTGGAATTCTCGGGTGCCAAGGAA5642491.3773314014614115RNA PCR Primer, Index 1 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC5494831.3412876061264103No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA4844751.1826031250795612No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA4636911.1318693960911643No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG4394661.0727361885880893No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA4033800.9846502886518261No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGTGGAATTCTC4029710.9836519199472334No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA3932860.9600108419423921No Hit
CCCCCCGAGTGTTACAGCCCCCCTGGAATTCTCGGGTGCCAAGGAACTCC3427100.8365548624717818RNA PCR Primer, Index 1 (100% over 27bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCA3223640.7868902911670318No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG3027170.7389319783574169Illumina Small RNA Adapter 2 (100% over 21bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA2975540.7263290990864829No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA2961840.722984930075989No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTC2915620.7117026314143085No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC2552530.623072388639111No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG2415760.5896868415175605Illumina Small RNA Adapter 2 (100% over 21bp)
GGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGG2295350.5602947692143807No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCTGGAATTCTCGGG2139890.5223469944427478No Hit
GATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGC2131900.5203966360198393No Hit
CTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGG2005430.4895253181543536No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCG1903110.4645490135445924No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTC1823110.44502101932273064No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG1821450.444615813442627Illumina Small RNA Adapter 2 (100% over 21bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG1775270.43334327877805723No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGG1766160.4311195284360427No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA1661280.4055183280111819No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGAATTCTCGGGTGCC1540600.3760603487275034No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC1489050.3634769974507912No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA1405010.3429628395207254No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG1353340.3303501962526804No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC1315140.3210255790117414No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCA1295740.31629004041293995No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA1284170.3134658042486032No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT1276900.3116911977736915No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCT1252770.30580106651652245No Hit
CATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGC1236160.30174656671620836No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG1215560.29671810820407896Illumina Small RNA Adapter 2 (100% over 21bp)
ATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCC1208410.29497279372050006No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAG1200300.2929931433062588No Hit
CTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1189480.29035198208775204RNA PCR Primer, Index 1 (100% over 32bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA1188580.2901322921527561No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCC1146980.27997773515738794No Hit
CAGGCTGATGAGACTAAGGCGAATGCGACTTGGAATTCTCGGGTGCCAAG1133720.27674097011511434No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG1115500.27229346943108534No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCC1048490.25593633327099835No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA1028390.2510299247227556No Hit
CGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGGAAC1027280.2507589738029272RNA PCR Primer, Index 1 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAATGGAATTCTCG1015500.2478834766537581No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTGGAATTCTCGGGTGCCAAGG1014040.24752709075920912Illumina Small RNA Adapter 2 (100% over 21bp)
ATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGT1012620.24718046886177109No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGTGGAATTCTCGG1006090.2455864963334116No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA982220.23975983105746357No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATGGAATTCTCGGGTG956330.23344008392741358No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATTCTCGGGTGCCAAGGA950540.2320267453456063RNA PCR Primer, Index 1 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGC940920.22967850404042744No Hit
ATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCGGGTG886360.2163604119811177No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCT847450.2068624837914597No Hit
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCTGGAATTCTCGGGT845400.20636207893952443No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGC844730.20619853198791638No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGCC835990.204065098619178No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGATGGAATTCTCGGGTGC832130.20312287289797315No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGTGGAATTCTCGGG812400.19830678132300647No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATTCTCGGGTGCCAAGG808230.19728888462419192Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC761370.1858503620087364No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC758540.18515955921313804No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC749950.1830627408335656No Hit
AGCAGAGTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAG748050.1825989509707964No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA735070.17943053390829933No Hit
CATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCGGGTGCC724600.17687480766451316No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC721040.1760058119216403No Hit
TGGAGGTGATGAACTGTCTGAGCCTGACCTGGAATTCTCGGGTGCCAAGG713630.17419703145684037Illumina Small RNA Adapter 2 (100% over 21bp)
CCCCCCGAGTGTTACAGCCCCCCCGGTGGAATTCTCGGGTGCCAAGGAAC686850.16766003539107213RNA PCR Primer, Index 1 (100% over 24bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG665780.16251684991288928No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA658000.16061775247481322No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCTGGAATTCTCGGGTGCCAAGG630170.15382445148488305Illumina Small RNA Adapter 2 (100% over 21bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGGAATTCTCG626980.15304577271528633No Hit
AGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA617950.15084155036749367RNA PCR Primer, Index 7 (100% over 35bp)
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCGGGTGCCA611530.14927442883118924No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATTGGAATTCTCGGGT611060.1491597018651358No Hit
GACCCAGTGGCCTAATGGATAAGGCATCAGCCTTGGAATTCTCGGGTGCC592530.1446365302034971No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAG566480.13827772708500333No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGA547240.13358124447464556RNA PCR Primer, Index 1 (100% over 22bp)
CGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGTGGAATTCTCGGGTGCCA537170.1311231582019687No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG530010.1293754027191121No Hit
GTTTCCGTAGTGTAGTGGTTATCACTTTCGCCTTGGAATTCTCGGGTGCC514700.1256382328249033No Hit
GCCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAAC513030.12523058594552192RNA PCR Primer, Index 1 (100% over 24bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTG511000.12473506309214218No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAA506390.1236097624251074No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCGTGGAATTCTCGGGTGCC467330.11407521924628337No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGCTGGAATTCTCG464160.1133014224752421No Hit
AGCAGAGTCGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA442290.10796295705484064No Hit
AACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCTGGAATTCTCGGG439100.1071842782852439No Hit
ACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCTGGAATTCTC422310.10308584049793065No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTTGGAATTCTCGG412980.10080838817180603No Hit
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGATGGAATTCTCGGGTGCC411550.10045932527509024No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)