FastQCFastQC Report
Tue 4 Jul 2023
SRR7633003.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7633003.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43542351
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG29169526.699114616020618No Hit
CCCCCCGAGTGTTACAGCCCCCTGGAATTCTCGGGTGCCAAGGAACTCCA24856445.7085663564652265RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC18259264.193448351008883No Hit
CCCCCCGAGTGTTACAGCCCCCCTGGAATTCTCGGGTGCCAAGGAACTCC11125862.555181276270544RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG9388252.156119222868788No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT8341471.9157141974258578No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA7427931.7059092652117014No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCTGGAATTCTCGGGTGCCAAGGAA6267971.439511155472519RNA PCR Primer, Index 1 (100% over 23bp)
TGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA5852701.344139640048375No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG5628001.292534709483188Illumina Small RNA Adapter 2 (100% over 21bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA4573681.0503980366149728No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA4556481.0464478594644557No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC4107990.9434469902647196No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA3955260.9083707951369001No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCA3530000.8107049617049846No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA3239920.7440847647385875No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCC3139910.7211163219000278No Hit
AGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC2823990.6485616727493654RNA PCR Primer, Index 6 (100% over 35bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA2671680.6135819354356865No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG2608740.5991270430023404Illumina Small RNA Adapter 2 (100% over 21bp)
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCC2011210.4618974294704482No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG1986860.4563051728649195Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC1897890.4358721925694825No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA1841290.4228733538067341No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCA1839710.42251048869639585No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA1838810.4223037933803804No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCTGGAATTCTCGGG1831100.42053310350651485No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGAATTCTCGGGTGCC1635870.37569629623352213No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATGGAATTCTCGGGTG1548290.3555825453705979No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC1504110.34543610196886243No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGATGGAATTCTCGGGTGC1472340.3381397573135176No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG1420160.32615602221386714No Hit
CGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGGAAC1389590.3191352713132095RNA PCR Primer, Index 1 (100% over 24bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGC1380480.31704305539220884No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGA1359320.3121834188512237RNA PCR Primer, Index 1 (100% over 22bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAG1331880.3058815083273753No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG1302000.2990192238356629Illumina Small RNA Adapter 2 (100% over 21bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA1252520.2876555746840587No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTGGAATTCTCGGGTGCCAAGG1240390.28486978114709516Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT1221380.2805039167499247No Hit
CCCCGCGGGGGCGCGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1200320.2756672463551635RNA PCR Primer, Index 1 (100% over 30bp)
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCTGGAATTCTCGGGT1196380.27476238019394034No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC1175800.27003594730105407No Hit
AGCAGAGTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAG1168230.26829741003190205No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG1143100.2625260174858266No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGC1076120.24714329274503344No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC1031550.23690728137302464No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA1025880.23560510088212736No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA1016830.23352666464886104No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG1006660.23119100757788666No Hit
TGGAGGTGATGAACTGTCTGAGCCTGACCTGGAATTCTCGGGTGCCAAGG964540.22151766678836426Illumina Small RNA Adapter 2 (100% over 21bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATTCTCGGGTGCCAAGGA928940.21334171873264263RNA PCR Primer, Index 1 (100% over 22bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA928420.21322229477227814No Hit
CGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGTGGAATTCTCGGGTGCCA915720.21030559420183811No Hit
GCCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAAC892900.20506471963353565RNA PCR Primer, Index 1 (100% over 24bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG887340.20378780190348475No Hit
CCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG859010.19728149267824333RNA PCR Primer, Index 6 (100% over 34bp)
CCCCCGCGGGGGCGCGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG855990.1965879150622804RNA PCR Primer, Index 1 (100% over 29bp)
AGTAAGGTCAGCTAAATAAGCTATCGGGCTGGAATTCTCGGGTGCCAAGG849610.19512267493319319Illumina Small RNA Adapter 2 (100% over 21bp)
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATTGGAATTCTCGGGT834110.19156292226848293No Hit
GTTTCCGTAGTGTAGTGGTTATCACTTTCGCCTTGGAATTCTCGGGTGCC820730.18849005190372015No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATTCTCGGGTGCCAAGG808960.18578693649316272Illumina Small RNA Adapter 2 (100% over 21bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTC767440.17625139258098396No Hit
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGATGGAATTCTCGGGTGCC749660.17216801178236793No Hit
GGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCA712960.16373943611818295RNA PCR Primer, Index 6 (100% over 37bp)
CTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG705470.16201927176601005RNA PCR Primer, Index 6 (100% over 34bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG687140.15780957716316238No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA685320.1573915933018867No Hit
TAGGATGGGTGTGATAGGTGGCACGGAGATGGAATTCTCGGGTGCCAAGG675480.15513172451345128Illumina Small RNA Adapter 2 (100% over 21bp)
AGCAGAGTCGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA670480.15398341720225442No Hit
TCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC656090.15067858876062984RNA PCR Primer, Index 6 (100% over 35bp)
GGTTCCATGGTGTAATGGTTAGCACTCTGGACTTGGAATTCTCGGGTGCC647950.14880914445800136No Hit
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCCA632360.14522872226168954No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTG626670.14392194854154752No Hit
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATGGAATTCTCGGGTGC615260.1413015112573963No Hit
TAGGATGGGGTGTGATAGGTGGCACGGAGATGGAATTCTCGGGTGCCAAG597110.13713315571775167No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGTGGAATTCTCGGGTGCCAAGGAAC596490.13699076561116325RNA PCR Primer, Index 1 (100% over 24bp)
GGGGATGTAGCTCAGTGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC586670.1347354900519726RNA PCR Primer, Index 1 (100% over 31bp)
CAGGCTGATGAGACTAAGGCGAATGCGACTTGGAATTCTCGGGTGCCAAG576610.13242509574184452No Hit
GACCCAGTGGCCTAATGGATAAGGCATCAGCCTTGGAATTCTCGGGTGCC575890.1322597394890322No Hit
TTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCC572120.13139391577638976RNA PCR Primer, Index 6 (100% over 36bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGC564760.12970360741430795No Hit
CATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGC553990.12723015346598993No Hit
AGCAGAGTAGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA547920.12583610839019693No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC532730.12234755077878087No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC525150.12060671689500643No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCG517560.11886358639660959No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC517490.11884751009425282No Hit
GCATGTTGGAACAATGTAGGTAAGGGAAGTCGGCTGGAATTCTCGGGTGC517090.11875564550935709No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAG510140.11715949834679344No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAG509310.11696887933313478No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAAC505910.11618803036152091RNA PCR Primer, Index 1 (100% over 24bp)
GGGGATGTAGCTCAGTGGTAGAGTGGAATTCTCGGGTGCCAAGGAACTCC502880.1154921561309356RNA PCR Primer, Index 1 (100% over 27bp)
CGCCGTCGGGCCCGGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA500400.11492259570458196RNA PCR Primer, Index 1 (100% over 32bp)
TGGATATGATGACTGATTACCTGAGATGGAATTCTCGGGTGCCAAGGAAC496760.11408662798203063RNA PCR Primer, Index 1 (100% over 24bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA478410.10987234014993814RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCGTGGAATTCTCGGGTGCC459060.1054283908556063No Hit
CTCGGATAGCCGGTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA457590.10509078850611442RNA PCR Primer, Index 1 (100% over 32bp)
TAGGATGGGTGTGATAGGTGGCACGGAGAATGGAATTCTCGGGTGCCAAG454930.10447988901655769No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG451710.10374037910814692No Hit
AGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA447500.10277350435211915No Hit
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG447390.10274824159127283RNA PCR Primer, Index 6 (100% over 34bp)
GGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAA440400.10114290797021962RNA PCR Primer, Index 1 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)