FastQCFastQC Report
Tue 4 Jul 2023
SRR7633006.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7633006.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42254368
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCG18698524.425227706636152No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTC15571033.6850699080388565No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG13759663.2563875999754632No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG10149112.401907892694076No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA9477022.2428497806427963No Hit
CCCCCCGAGTGTTACAGCCCCCTGGAATTCTCGGGTGCCAAGGAACTCCA9238522.1864059119284422RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA7988831.8906518729613944No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCTGGAATTCTCGGGTGCCAAGGAA7723351.8278228655555802RNA PCR Primer, Index 1 (100% over 23bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT6892291.631142607552431No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC5460561.2923066320622758No Hit
CCCCCCGAGTGTTACAGCCCCCCTGGAATTCTCGGGTGCCAAGGAACTCC5419411.2825679939172208RNA PCR Primer, Index 1 (100% over 27bp)
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTC5389431.2754728694557684No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC4733821.1203149459009776No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA4376021.0356373097332803No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC3653310.8645993711230043No Hit
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG3199680.7572424228425331Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC3077410.7283057694768976No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCTGGAATTCTCGGG2967680.7023368566298283No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA2930070.6934360016933634No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT2884980.6827649155703855No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGA2842360.6726783843980343RNA PCR Primer, Index 1 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG2837840.6716086725045799No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA2810640.6651714681899868No Hit
GATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGC2667500.6312956804844413No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCG2469680.584479218811177No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG2280550.539719349251656No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA2169900.5135327074351225No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG2156150.5102786059893264Illumina Small RNA Adapter 2 (100% over 21bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGGAATTCTCG2134820.5052306071646842No Hit
CTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGG2009170.4754940364981911No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA2005300.4745781548549016No Hit
CTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1989740.4708956953278771RNA PCR Primer, Index 1 (100% over 32bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA1892150.4477998582300415No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA1851490.4381771844274182No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCC1826790.4323316349211518No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTTGGAATTCTC1725790.4084287806647587No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA1705300.40357957785571424No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGG1702160.40283645941645607No Hit
ATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCC1662930.3935522121641956No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCC1640340.38820601931615684No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTTGGAATTCTCGG1531700.36249506796551784No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTC1499140.35478935574187265No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGTGGAATTCTC1489460.3524984683240322No Hit
GGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGG1466290.3470150115604616No Hit
CGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCTGGAATTCTCG1452570.34376800997236545No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCA1436310.3399198870990095No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGAATTCTCGGGTGCC1375530.32553557539897415No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATGGAATTCTCGGGTG1356540.32104136547492557No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTGGAATTCTCGGGTGCCAAGG1353250.3202627477471678Illumina Small RNA Adapter 2 (100% over 21bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATGGAATTC1334380.31579693725391894No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGTGGAATTCTCGG1247640.2952688820242205No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGATGGAATTCTCGGGTGC1245110.2946701273581941No Hit
ACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCTGGAATTCTC1212300.2869052496537163No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCT1114770.2638236122712804No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA1075070.25442813391505464No Hit
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCTGGAATTCTCGGGT1053710.24937303523271248No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT1049060.2482725572892251No Hit
AACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCTGGAATTCT1018660.2410780348199741No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGTGGAATTCTCGGGTGCCAAGGAAC985870.23331789035396294RNA PCR Primer, Index 1 (100% over 24bp)
AACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCTGGAATTCTCGGG984550.2330054966151665No Hit
GGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAA956410.22634583009264272RNA PCR Primer, Index 1 (100% over 23bp)
CAGGCTGATGAGACTAAGGCGAATGCGACTTGGAATTCTCGGGTGCCAAG942330.2230136302121475No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC914240.2163657967857903No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGCC889840.21059124585652306No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATTGGAATTCTCGGGT849970.20115553497333105No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCTGGAATTCTCGGGT837100.1981096960200659No Hit
CCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT832310.1969760854073122RNA PCR Primer, Index 3 (100% over 34bp)
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGT809040.19146896245140857No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA808280.1912890993896773No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCGGGTGCCA808110.1912488668627111No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG807910.19120153447804497Illumina Small RNA Adapter 2 (100% over 21bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGTGGAATTCTCGGG807660.1911423689972123No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC806980.1909814388893475No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATTCTCGGGTGCCAAGGA789530.1868516883272281RNA PCR Primer, Index 1 (100% over 22bp)
CGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGTGGAATTCTCGGGTGCCA788540.18661739302313077No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG779970.18458920034018733Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC777310.18395967962412788No Hit
ATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGT764380.18089964095546288No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGC762560.18046891625500114No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG762040.18034585205486922No Hit
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT752440.17807389759089523RNA PCR Primer, Index 3 (100% over 34bp)
ATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCGGGTG741700.1755321485343243No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG718040.16993272742832172No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGTGGAATTCTCG708070.16757320805271542No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAA707470.16743121089871701No Hit
TTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA698640.16534148611570762RNA PCR Primer, Index 3 (100% over 36bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTG674900.15972313205583857No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG668130.15812093083489026Illumina Small RNA Adapter 2 (100% over 21bp)
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCTGGAATTCTCGGGT666290.15768547289596188No Hit
TGGAGGTGATGAACTGTCTGAGCCTGACCTGGAATTCTCGGGTGCCAAGG647580.15325752831044592Illumina Small RNA Adapter 2 (100% over 21bp)
GTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAAGGAA645510.15276763812915153RNA PCR Primer, Index 1 (100% over 23bp)
GAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCTGGAATTCTCGG643130.15220438275162462No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGC631820.14952773639875527No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAG628200.1486710202362984No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA610470.14447500433564645No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCTGGAATTCTCGGGT577600.1366959269157688No Hit
TAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCA574460.13595280847651064No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCT572130.1354013861951503No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG570460.13500616078318814Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC570350.13498012797162176No Hit
CGAGAATTAATGTGAATTGCAGGACTGGAATTCTCGGGTGCCAAGGAACT569480.13477423209832415RNA PCR Primer, Index 1 (100% over 25bp)
CATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGC559770.13247624482278375No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAG556720.13175442595662537No Hit
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGATGGAATTCTCGGGTGCC555090.13136866702159644No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG549460.13003626039324503No Hit
GACCCAGTGGCCTAATGGATAAGGCATCAGCCTTGGAATTCTCGGGTGCC536350.12693362257838053No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATTCTCGGGTGCCAAGG528160.12499536142630271Illumina Small RNA Adapter 2 (100% over 21bp)
CCCCGCGGGGGCGCGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT525430.12434927437561011RNA PCR Primer, Index 1 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC514420.12174362659973993No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTTGGAATTCTCGGGTG510300.12076857947561775No Hit
AGCAGAGTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAG500790.11851792458474353No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCA479210.11341076027926865No Hit
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATGGAATTCTCGGGTGC459150.10866332209725632No Hit
CGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGGAAC456910.10813319938899571RNA PCR Primer, Index 1 (100% over 24bp)
CTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT454430.10754627781913575RNA PCR Primer, Index 3 (100% over 35bp)
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA440590.10427087680023991No Hit
CTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT440110.10415727907704123RNA PCR Primer, Index 3 (100% over 34bp)
GGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTG433160.10251247870989338No Hit
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCCA430540.10189242447076714No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCCGACTGGAATTCT429110.10155399792040436No Hit
CCGCAGCTAGGAATAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT425470.10069254851948087RNA PCR Primer, Index 3 (100% over 34bp)
AGTAAGGTCAGCTAAATAAGCTATCGGGCTGGAATTCTCGGGTGCCAAGG424990.10057895079628218Illumina Small RNA Adapter 2 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)