FastQCFastQC Report
Tue 4 Jul 2023
SRR7633008.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7633008.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39900006
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG19782304.957969179252755No Hit
CCCCCCGAGTGTTACAGCCCCCTGGAATTCTCGGGTGCCAAGGAACTCCA19457944.876675958394593RNA PCR Primer, Index 1 (100% over 28bp)
CCCCCCGAGTGTTACAGCCCCCCTGGAATTCTCGGGTGCCAAGGAACTCC9680772.426257780512614RNA PCR Primer, Index 1 (100% over 27bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT9157692.2951600558656557No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC8632012.1634107022440046No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC8287082.0769620936899105No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT6437571.6134258225424827No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG6207241.5556990141805993No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG5189421.300606320710829No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA5099451.2780574519211851No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA5032321.2612328930476853No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA4775611.1968945568579614No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG4611361.1557291495144137Illumina Small RNA Adapter 2 (100% over 21bp)
CCCCCCGAGTGTTACAGCCCCCCCGGCTGGAATTCTCGGGTGCCAAGGAA4344711.088899585629135RNA PCR Primer, Index 1 (100% over 23bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA3855180.9662103810209953No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCA3180570.7971352184759071No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCC3065720.7683507616515145No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG2396820.6007066765854622Illumina Small RNA Adapter 2 (100% over 21bp)
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGATGGAATTCTCGGGTGC2271230.5692304908425326No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATGGAATTCTCGGGTG2155610.5402530515910198No Hit
AGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT2066200.5178445336574636RNA PCR Primer, Index 1 (100% over 35bp)
CGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGGAAC2056550.51542598765524RNA PCR Primer, Index 1 (100% over 24bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA1987680.4981653386217536No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC1903220.4769974220054002No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCC1838260.4607167226992397No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA1786940.44785456924492695No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA1766120.4426365249168133No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA1540140.38599994195489595No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC1532300.3840350299696697No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCA1444630.3620626021960999No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG1432770.35909017156538775Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC1430810.3585989435690812No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGA1425990.357390923700613RNA PCR Primer, Index 1 (100% over 22bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG1414430.3544936810285192Illumina Small RNA Adapter 2 (100% over 21bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTG1406620.3525362878391547No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA1401190.35117538578816254No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGAATTCTCGGGTGCC1308170.32786210608589883No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCTGGAATTCTCGGG1304750.3270049633576496No Hit
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCTGGAATTCTCGGGT1130480.2833282781962489No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA1073170.26896487183485634No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGC1058880.2653834187393356No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAG1029000.25789469806094767No Hit
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGATGGAATTCTCGGGTGCC1007480.25250121516272456No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGC979560.24550372248064323No Hit
CGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGTGGAATTCTCGGGTGCCA971630.24351625410783148No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATTGGAATTCTCGGGT925100.23185460172612504No Hit
GCCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAAC885250.22186713455632062RNA PCR Primer, Index 1 (100% over 24bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG882510.22118041786760634No Hit
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCCA840740.21071174776264445No Hit
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATGGAATTCTCGGGTGC808140.20254132292611685No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA804560.20164407995327116No Hit
CCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA798680.2001703959643515RNA PCR Primer, Index 1 (100% over 34bp)
CGCTGAGAAGACGGTCGAACTTGACTATCTGGAATTCTCGGGTGCCAAGG787760.19743355427064346Illumina Small RNA Adapter 2 (100% over 21bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC759770.19041851773155122No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCC759360.1903157608547728No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCTGGAATTCTCGGGT734640.1841202730646206No Hit
GCATGTTGGAACAATGTAGGTAAGGGAAGTCGGCTGGAATTCTCGGGTGC731850.18342102504946992No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAAC703240.1762506000625664RNA PCR Primer, Index 1 (100% over 24bp)
CCTCTGGCATGTTGGAACAATGTAGGTAAGGGATGGAATTCTCGGGTGCC701400.17578944724970716No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC672020.16842603983568322No Hit
ATTGATCATCGACACTTCGAACGCACTGGAATTCTCGGGTGCCAAGGAAC669300.1677443356775435RNA PCR Primer, Index 1 (100% over 24bp)
CCCCGCGGGGGCGCGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT664730.16659897244125726RNA PCR Primer, Index 1 (100% over 30bp)
TGGAGGTGATGAACTGTCTGAGCCTGACCTGGAATTCTCGGGTGCCAAGG643970.1613959657048673Illumina Small RNA Adapter 2 (100% over 21bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG623980.1563859413955978No Hit
GGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAAC617150.15467416220438665RNA PCR Primer, Index 1 (100% over 24bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGC614980.15413030263704722No Hit
CGCGACCTCAGATCAGACGTGGCGACCCTGGAATTCTCGGGTGCCAAGGA607880.15235085428307954RNA PCR Primer, Index 1 (100% over 22bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATTCTCGGGTGCCAAGGA597730.14980699501649197RNA PCR Primer, Index 1 (100% over 22bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA591410.14822303535493203No Hit
GGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAA583800.14631576747131317RNA PCR Primer, Index 1 (100% over 23bp)
TAGGATGGGTGTGATAGGTGGCACGGAGATGGAATTCTCGGGTGCCAAGG576550.14449872513803633Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG574810.14406263497804989No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCTGGAATTCTCGGGTGCCAAGG570870.14307516645486218Illumina Small RNA Adapter 2 (100% over 21bp)
AGCAGCCGACTTAGAACTGGTGCGGACCAGGTGGAATTCTCGGGTGCCAA558560.13998995388622248No Hit
ATTGATCATCGACACTTCGAACGCATGGAATTCTCGGGTGCCAAGGAACT534960.13407516780824544RNA PCR Primer, Index 1 (100% over 25bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG531770.13327566918160363No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA506540.1269523618618002No Hit
TAGGATGGGGTGTGATAGGTGGCACGGAGATGGAATTCTCGGGTGCCAAG504690.12648870278365373No Hit
TTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATC482170.12084459335670275RNA PCR Primer, Index 1 (100% over 36bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG480350.12038845307441809Illumina Small RNA Adapter 2 (100% over 21bp)
GCATGTTGGAACAATGTAGGTAAGGGATGGAATTCTCGGGTGCCAAGGAA476900.11952379155030703RNA PCR Primer, Index 1 (100% over 23bp)
CCCCCCGAGTGTTACAGCCCCCCCGGTGGAATTCTCGGGTGCCAAGGAAC474930.11903005728871319RNA PCR Primer, Index 1 (100% over 24bp)
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATTGGAATTCTCGGGTG462380.11588469435317879No Hit
CGGTGGATCACTCGGCTCGTGCGTCGATGTGGAATTCTCGGGTGCCAAGG461470.11565662421203644Illumina Small RNA Adapter 2 (100% over 21bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGTGGAATTCTCGGG455200.1140851958769129No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATTCTCGGGTGCCAAGG454470.11390223851094158Illumina Small RNA Adapter 2 (100% over 21bp)
AGCAGAGTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAG446680.1119498578521517No Hit
CGTCCAGTGCGGTAACGCGACCGATCCCGGATGGAATTCTCGGGTGCCAA439270.1100927152742784No Hit
ATTGATCATCGACACTTCGAACGCACTTGGAATTCTCGGGTGCCAAGGAA434490.10889472046695933RNA PCR Primer, Index 1 (100% over 23bp)
GGGGATGTAGCTCAGTGGTAGAGTGGAATTCTCGGGTGCCAAGGAACTCC425950.10675436991162356RNA PCR Primer, Index 1 (100% over 27bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG425170.10655888121921585No Hit
AGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGGAATTCTCGGGTGC424050.10627817950704067No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC422830.10597241514199271RNA PCR Primer, Index 1 (100% over 24bp)
TCCCTGGTGGTCTAGTGGTTAGGATTGGAATTCTCGGGTGCCAAGGAACT417110.10453883139766947RNA PCR Primer, Index 1 (100% over 25bp)
CCCCCGCGGGGGCGCGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG416340.10434584897054902RNA PCR Primer, Index 1 (100% over 29bp)
AGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA413960.10374935783217676No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG413040.10351878142574715No Hit
GGCATGTTGGAACAATGTAGGTAAGGGAAGTCGGCTGGAATTCTCGGGTG411490.103130310306219No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAG407080.10202504731452924No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC405410.10160650101155373No Hit
CGACTCTTAGCGGTGGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC401490.1006240450189406RNA PCR Primer, Index 1 (100% over 31bp)
CTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT401160.1005413382644604RNA PCR Primer, Index 1 (100% over 35bp)
ATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCGGGTG401090.10052379440744945No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)