FastQCFastQC Report
Fri 7 Jul 2023
SRR7640000.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7640000.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27184056
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGACGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA24567229.037363666407986Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG21327187.845473832161029No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG12407814.564370379460666TruSeq Adapter, Index 9 (100% over 49bp)
TGGGGGGGACGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGA6228282.2911518428302236Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGGGACGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA6183062.2745170919306523Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT5409941.9901150880501424No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT4820421.773252674288193No Hit
CGGGGGCCCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTG4642411.7077694366138738Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCGGTTGGCCCCGGATAGCCGGCTCCCCGAGATCGGAAGAGCACACGT3100471.1405472384253477No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGTCCGCAGATCGGAAGAGCAC2952391.0860741311009658No Hit
CGGGGGCCCGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCTGA2886701.0619092309109428Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC2316590.852187031986691No Hit
GGGGGGGACGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAAC2304150.8476108201071981Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCTCGGTTGGCCCCGGATAGCCGGCTCCCCGAGATCGGAAGAGCACACG2237220.8229897701799908No Hit
TTGGGGGGGACGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA2230850.8206464848365527Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGGGGACGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA1967590.7238029527308214Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT1943630.714988962647811No Hit
CGCGGGCGGGCGCGACCCGCTCCGGGGACAGATCGGAAGAGCACACGTCT1868760.6874470829518597Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGGACGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGA1590510.5850892891038777Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC1487580.547225182290678No Hit
TGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA1376180.5062452784823575Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGTCCGAGATCGGAAGAGCACA1364880.502088430070921No Hit
CGGCAGTCGGAACGGGACGGGAGCGGCCGCGGGCAGATCGGAAGAGCACA1212080.44587901084370923No Hit
CGGGTGCGGGGCGGGTCCCCCCGCCGGGCGTAGATCGGAAGAGCACACGT1110350.4084563392600427No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGCCCGTCCGCAGATCGGAAGAGCACA1019360.3749845129806972No Hit
AGCCTCGGTTGGCCCCGGATAGCCGGCTCCCCGAGATCGGAAGAGCACAC885080.32558791079594596No Hit
CGGCAGTCGGAACGGGACGGGAGCGGCCGCGGGAGATCGGAAGAGCACAC835370.3073014564125383No Hit
AAGTGGCGTTCAGCCACCCGAGATTGAGCAGATCGGAAGAGCACACGTCT817630.3007755722692743Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGCTCTCGCTTCTGGCGCCAAGCGCCCGTCCGCGAGATCGGAAGAGCAC787810.2898059068153774No Hit
CGCGGGCGGGCGCGACCCGCTCCGGGGACAGTGCCAGATCGGAAGAGCAC787690.2897617632924241No Hit
CGGGTGCGGGGCGGGTCCCCCCGCCGGGCGAGATCGGAAGAGCACACGTC776220.28554237822347045No Hit
TGGGGGGGACGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCTG760890.27990304316618536Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGTGGCGCAATGAAGGTGAAGGGCCAGATCGGAAGAGCACACGTCTGAA756460.2782734114438257Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC640820.2357337698244883TruSeq Adapter, Index 9 (100% over 50bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC614940.22621348337422495No Hit
CTCGGTTGGCCCCGGATAGCCGGCTCCCCGAGATCGGAAGAGCACACGTC613860.22581619166764516No Hit
CCTCGGTTGGCCCCGGATAGCCGGCTCCAGATCGGAAGAGCACACGTCTG613670.22574629775630245Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAAGAGCACACGTCTGA599660.22059254145150378Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGAGAGGTGTAGAATAAGTGGGAGGCCCAGATCGGAAGAGCACACGTCT596530.219441131227805Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGACGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGAA596480.21942273809324114Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCCGCCCCTTGCCTCTCGGCGCCCCAGATCGGAAGAGCACACGTCTGAA589070.21669687555087436Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGTCCGCGAGATCGGAAGAGCA565400.20798956564833446No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC539730.1985465303632394TruSeq Adapter, Index 9 (100% over 50bp)
TTGGGGGGGACGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTG524200.19283362276769883Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGGGGACGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACTC522950.1923737944036019Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGCTTCTGGCGCCAAGCGCCCGTCCGAGATCGGAAGAGCACACGTCTG512510.18853330790666412Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGAGATCGGAAGAGCACACGTCT495850.1824047154699799Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGATCGGAAGAGCAC479610.17643062536363227No Hit
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA475220.17481570814892378Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCG470990.17325964896481966TruSeq Adapter, Index 9 (100% over 50bp)
CGGGGGCCCGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTCT459140.16890047607318054Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGGGACGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTG458240.16856939965103074Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG448890.1651298834875855No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGCTCCCCGAGATCGGAAGAGCAC442080.16262473855998533No Hit
TGGGGGGGACGCGTGCATTTATCAGAGATCGGAAGAGCACACGTCTGAAC433950.15963401487989873Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCTCGGTTGGCCCCGGATAGCCGGCTAGATCGGAAGAGCACACGTCTGAA412590.15177646779420995Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC412150.15161460821004782No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG411540.15139021196836852No Hit
GCCTCGGTTGGCCCCGGATAGCCGGCTCCAGATCGGAAGAGCACACGTCT408650.15032708879057635Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGTGGGGAGTTTGACTGGGGCGGTACAGATCGGAAGAGCACACGTCTGA399310.1468912512540439Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGCTTCTGGCGCCAAGCGCCCGTCCGCAGATCGGAAGAGCACACGTCT398720.14667421226619015Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC383680.1411415573893756No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACG366630.13486949850309313No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGT362430.13332447519972737TruSeq Adapter, Index 9 (100% over 50bp)
TATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATC351790.12941041616453408TruSeq Adapter, Index 9 (100% over 42bp)
CCTCGGTTGGCCCCGGATAGCCGGCTCCCAGATCGGAAGAGCACACGTCT348320.12813393262580094Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGAGATCGGAAGAGCACACGTCTG344830.12685009183324225Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA344460.12671398263746955No Hit
CGGTCGCGGGGGCCGGGCCGCCCCTCCCAGATCGGAAGAGCACACGTCTG338700.1245950935357108Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGAGATCGGAAGAGCAC337280.12407272851409665No Hit
AGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCAGATCGGAAGAGCACAC330180.12146090340602593No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGAGATCGGAAGAGCACACGTCTGAA328640.1208943948614585Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGA313910.11547577741893998Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGGTTGGCCCCGGATAGCCGGCTCCAGATCGGAAGAGCACACGTCTGA313330.11526241705799899Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCCCCGGGGCTCCCCCCGCGGCGTCGGGAGATCGGAAGAGCACACGTCT306810.11286395231086929Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCTTCTGGCGCCAAGCGCCCGTCCGCAGATCGGAAGAGCACACGTCTG280790.10329216508382709Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGGACGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA279320.10275140692764906Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGAGATCGGAAGAGCACACGT277230.10198257390287896No Hit
CGGGGGCCCGGGGGGCGGGGCGGACTGTCCAGATCGGAAGAGCACACGTC273410.10057733842219867No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)