FastQCFastQC Report
Fri 7 Jul 2023
SRR7640002.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7640002.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30781009
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGACGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA347509711.28974362081503Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG310978510.102933922666407TruSeq Adapter, Index 11 (100% over 49bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG19229526.2472026176919675No Hit
GGGGGGGGACGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA13901984.516414650344958Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGGGGGGACGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGA6933392.2524895139077477Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGGACGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAAC6086021.9771996428057312Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT4836301.5711960579329936No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT3925001.2751368871631206No Hit
GGGGGGGGGACGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA3921331.273944593564168Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGGGGGGACGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA3268761.0619404971422477Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGGGCCCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTG3134821.0184266539150812Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGGGCCCGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCTGA2859030.9288292011480195Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGGGACGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGA2819030.9158341755463573Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAG2161180.7021147357450173Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGTCCGCAGATCGGAAGAGCAC1989880.6464635386058982No Hit
CCTCGGTTGGCCCCGGATAGCCGGCTCCCCGAGATCGGAAGAGCACACGT1613700.5242518203350645No Hit
TGGGGGGGACGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACTC1611810.5236378053753858Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC1542960.5012701175585245TruSeq Adapter, Index 11 (100% over 50bp)
GCCTCGGTTGGCCCCGGATAGCCGGCTCCCCGAGATCGGAAGAGCACACG1505460.48908728105696603No Hit
CGGGGAAGGTGGCTCGGGGGGGGAGATCGGAAGAGCACACGTCTGAACTC1448470.4705726183309975Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGGGCGGGCGCGACCCGCTCCGGGGACAGATCGGAAGAGCACACGTCT1244470.4042979877625194Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGACGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGAA1150570.373792165162617Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC1146720.372541393948457No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC1131540.3676097817326261TruSeq Adapter, Index 11 (100% over 50bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGTCCGAGATCGGAAGAGCACA1112120.3613006968030191No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT1111900.3612292241622099No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCG1029870.33457967540960076TruSeq Adapter, Index 11 (100% over 50bp)
GGGCTCTCGCTTCTGGCGCCAAGCGCCCGTCCGCAGATCGGAAGAGCACA969010.31480774395667144No Hit
CGGCAGTCGGAACGGGACGGGAGCGGCCGCGGGAGATCGGAAGAGCACAC842350.27365899538900756No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC839040.27258365702047No Hit
GGGGGGGGGACGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTG793690.2578505467445853Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGCGCGCGCCGCGGCTGGACGAGGAGATCGGAAGAGCACACGTCTGAA760310.24700619787999803Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGT750720.24389064049199943TruSeq Adapter, Index 11 (100% over 50bp)
ACGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC718030.2332704558190409Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATGAGAGGTGTAGAATAAGTGGGAGGCCCAGATCGGAAGAGCACACGTCT701260.22782229133554394Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGCAGTCGGAACGGGACGGGAGCGGCCGCGGGCAGATCGGAAGAGCACA689110.22387505230903898No Hit
GGGGGGACGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAACT662710.2152983354119418Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCCGCCCCTTGCCTCTCGGCGCCCCAGATCGGAAGAGCACACGTCTGAA639550.2077742155885793Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTC631670.20521419554505183TruSeq Adapter, Index 11 (100% over 50bp)
TGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA611930.19880115041063146Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGACGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAACTC602220.1956466079458279Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGGGGGGGACGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTG599810.19486365765332775Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGCTCTCGCTTCTGGCGCCAGATCGGAAGAGCACACGTCTGAACTCCA597320.19405471730962426Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGT593840.19292415008227964Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATC588200.19109185147244523TruSeq Adapter, Index 11 (100% over 42bp)
TGGGGGGGACGCGTGCATTTATCAGAGATCGGAAGAGCACACGTCTGAAC557110.18099146782355316Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCCCCGGATAGCCGGCTCCCCGAGATCGGAAGAGCACACGTCTGAACTC539250.1751891888924109Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGGGGGACGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACTC518360.16840253677194272Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGCTCTCGCTTCTGGCGCCAAGCGCCCGTCCGCGAGATCGGAAGAGCAC517970.16827583527232653No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC507710.1649426112055001No Hit
ACCCCGCCCCGAGTGTTACAGCCCCCCGGAGATCGGAAGAGCACACGTCT502870.16337021310769897Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGGGACGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCTG500790.16269447177641252Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGTGGCGTTCAGCCACCCGAGATTGAGCAGATCGGAAGAGCACACGTCT481380.15638863560320587Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCTCGGTTGGCCCCGGATAGCCGGCTCCCCGAGATCGGAAGAGCACAC476060.15466029719818478No Hit
TGGGGGGGACGCGTGCATTTAGATCGGAAGAGCACACGTCTGAACTCCAG453790.14742531669445924Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGGGGGGGACGCGTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC449550.14604784398068302Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGATCGGAAGAGCAC425990.1383937739013039No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGTCCGCGAGATCGGAAGAGCA420850.13672391311149026No Hit
TGGGCGCGCGCCGCGGCTGGACGAGAGATCGGAAGAGCACACGTCTGAAC400360.13006721124703874Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCCCCGGGGCTCCCCCCGCGGCGTCGGGAGATCGGAAGAGCACACGTCT394980.12831938030361512Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACG390310.12680221106462106No Hit
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAGG366880.11919037481844731No Hit
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA365010.1185828573715696Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAAGAGCACACGTCTGA361860.11755949910543868Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGGGCGGGCGCGACCCGCTCCGGGGACAGTGCCAGATCGGAAGAGCAC359260.11671482244133063No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACA352770.11460637953746092No Hit
GGGGACGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAACTCC352010.11435947405102932Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCCGCCCCTTGCCTCTCGGCGCCCAGATCGGAAGAGCACACGTCTGAAC349930.11368373271974289Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGGGGGCGGTCGCGGGGGCCGGGCCGAGATCGGAAGAGCACACGTCTGA346810.11267012072281321Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGAGATCGGAAGAGCACACGTCT342700.11133488184224241Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTGGCGCAATGAAGGTGAAGGGCCAGATCGGAAGAGCACACGTCTGAA324510.10542539394988644Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGCCCGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCTGAAC312270.10144891611577776Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)