FastQCFastQC Report
Fri 7 Jul 2023
SRR7640013.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7640013.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40138878
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG24539376.113616329783808No Hit
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA16762754.1761879841285054Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA15582463.8821364164688412Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC11753242.928143631717857No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG9474072.3603225780252255No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCAGATCGGAAGAGCACACGTCTGAA7661941.9088575420568556Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT7445001.8548101917547373No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC6133641.5281044975895937No Hit
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAAC5996091.493835976182493Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA5513041.3734913068571573No Hit
GGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGA4311311.0740982844612648Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTG4188161.0434173072799893Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC3913350.974952513620336No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG3884170.9676827538627263No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC3339760.8320511599751245No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG3016360.7514808959034679No Hit
GGGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTG2986110.7439445616790784Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCT2571680.640695537129862Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGAA2492310.6209216909351577Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT2199330.5479301140405569No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG1994680.4969446330811738TruSeq Adapter, Index 3 (100% over 49bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC1877720.4678058016469718No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT1843310.4592330657573438No Hit
CGTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA1794250.4470105018879701No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG1724220.42956357673973844No Hit
CGTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC1696840.4227422600103571No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC1660700.4137385205435986No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACAC1625580.4049888987928362No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT1593960.39711124959696187Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA1468400.365829857027892Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT1429060.3560288855109503No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACA1427730.3556975359400928No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACG1409340.3511159430016953No Hit
TGCGTGCATTTATCAGATCAAAACCAAAGATCGGAAGAGCACACGTCTGA1408940.35101628899542237Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCTG1375990.3428072902286905Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT1313470.327231369048233No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCT1283620.3197946888301163Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCAGATCGGAAGAGCACACGTCTGA1267930.31588576043406097Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCCGCCCCTTGCCTCTCGGCGCCCCAGATCGGAAGAGCACACGTCTGAA1240700.3091018139570319Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTG1215480.3028186288615242Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGTC1197500.29833918127955644No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACG1154770.2876936420594517No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTG1080290.26913806609143387Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA1058960.26382401620693036No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCAC1021010.254369342361787No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC941500.2345606172648872No Hit
CCCCGCCCCTTGCCTCTCGGCGAGATCGGAAGAGCACACGTCTGAACTCC930030.23170303863501118Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTC925620.23060435321585224No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA891130.22201168652496964No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTC870770.21693929760567798No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCAGATCGGAAGAGCACACGT865690.2156736917260119No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA860880.21447535230057999No Hit
GGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACGTC828410.20638593834137564No Hit
ATGCGTGCATTTATCAGATCAAAACCAAAGATCGGAAGAGCACACGTCTG819570.20418358480274412Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCT812540.20243216564249755Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCTGA809950.20178690595188037Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGCCCGTCACGTTGAACGCACGTTCGTAGATCGGAAGAGCACACGTCT806680.20097223445059925Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG756450.18845818261287722Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCCGCCCCTTGCCTCTCGGCGCCCAGATCGGAAGAGCACACGTCTGAAC750380.18694593306768564Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGGGGATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACGT746300.18592946220370185No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTTAGATCGGAAGAGCACACGTCTG737490.18373458271554077Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCAACTTCTTAGAGGGACAAGTGGCGTAGATCGGAAGAGCACACGTCTGA729500.18174399394023918Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGAGATCGGAAGAGCAC728300.18144503192142045No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCAGATCGGAAGAGCACACGTCTG724170.18041610430665253Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGATGCGTGCATTTATCAGATCAAAACCAGATCGGAAGAGCACACGTC715890.17835326637680304No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCAC700770.17458634493968667No Hit
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACACG700310.1744717428324728No Hit
TGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT678960.16915271024765566No Hit
GGGGATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACGTCTG678320.16899326383761898Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGGGGATGCGTGCATTTATCAGAGATCGGAAGAGCACACGTCTGAA674460.1680316026770853Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT672780.16761305585073905No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCACA664920.16565485462747614No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACGT645500.16081665262292583No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTCTG641220.1597503547558056Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAACT640090.15946883218808458Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATGCGTGCATTTATCAGATCAAAACCAAGATCGGAAGAGCACACGTCTGA597940.14896779127707555Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGGAAGGTGGCTCGGGGGGGGAGATCGGAAGAGCACACGTCTGAACTC592970.1477295902491345Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCT571270.1423233604088286Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCT561410.13986688915420106Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCTG561070.1397821832488691Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACGT547320.13635657678323745No Hit
TGGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTCTGAACTCCA537020.1337904861217097Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTG527950.13153083152947126Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCAGATCGGAAGAGCACACGTC519280.1293708309435057No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG512440.12766674743623874Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTAAACCATTCGTAGACGACCTGCTTCAGATCGGAAGAGCACACGTCTGA510850.12727062276130388Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCGTGCATTTATCAGATCAAAACCAAGATCGGAAGAGCACACGTCTGAA509320.12688944618730996Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTG495960.12356100237779441Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGCAGATCGGAAGAGC493180.12286840703419763No Hit
GGGGGATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACGTCT491640.12248473911004687Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGATGCGTGCATTTATCAGATCAAAACCAGATCGGAAGAGCACACGT488390.12167505030907941No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC487010.1213312439874378No Hit
CCCCGCCCCTTGCCTCTCGGCGCCAGATCGGAAGAGCACACGTCTGAACT480370.11967698748330732Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC468020.1166001700396309No Hit
GGGGATGCGTGCATTTATCAGATCAAAACCAAGATCGGAAGAGCACACGT462130.11513276479726214No Hit
GGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGAAC456550.1137425914097549Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACA425980.10612653398034692No Hit
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGAGATCGGAAGAGCACACGT413300.10296750198149536No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACA410040.10215532183037106No Hit
CGGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCT401920.10013234550303077Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)