FastQCFastQC Report
Fri 7 Jul 2023
SRR7640015_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7640015_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28127235
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length85
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC13062494.644071839980005No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC11298024.016754579680513No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG8600763.0578050064288225No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA6725782.3911984238763604No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCT5620221.9981416587872929Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG4617341.641590437168815No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG4569441.6245606793557916No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG3905391.3884727738080191No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT3627601.2897108443115721No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCAGATCGGAAGAGCACACGTCTGAA3618211.2863724429365346Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT3332761.1848871742992155No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACG3275731.1646114522099311No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC3274161.1640532743442433No Hit
GGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTG3211211.1416728306212822Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC2948301.0482011473932649No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC2671760.9498836270255502No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCT2572670.9146544265726795Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTC2244710.7980556922854308No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCAGATCGGAAGAGCACACGTCTGA2189620.7784696931639388Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCGTGCATTTATCAGATCAAAACCAAAGATCGGAAGAGCACACGTCTGA2174290.7730194596091653Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGCGTGCATTTATCAGATCAAAACCAAAGATCGGAAGAGCACACGTCTG1881380.6688819572915717Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTCTG1875670.6668518963915223Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACA1769500.6291055626335116No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCAGATCGGAAGAGCACACGTCTGAA1641080.5834487463840651Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA1594040.5667247420516095Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGATGCGTGCATTTATCAGATCAAAACCAGATCGGAAGAGCACACGTC1436720.510793186745871No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCAC1432310.5092253113397033No Hit
CGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTG1344090.4778606926702891Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGCGTGCATTTATCAGATCAAAACCAAGATCGGAAGAGCACACGTCTGA1215310.4320758865917677Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCAGATCGGAAGAGCACACGTC1209510.4300138282344496No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCAGATCGGAAGAGCACACGT1190500.4232552542046881No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCAGATCGGAAGAGCACACGTCTG1186730.42191491627243133Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGTC1165210.4142639687121752No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACG1162650.4133538188165314No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCTG1092880.3885486788872067Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG1088270.38690969802044173No Hit
CGTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA1005170.35736537914231525No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC997820.3547522534653691No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT959540.34114266830706963No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC949140.3374451843560165No Hit
TGCGTGCATTTATCAGATCAAAACCAAGATCGGAAGAGCACACGTCTGAA927850.32987600807544715Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGCGTGCATTTATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTCT923600.32836501703775717Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC893070.31751076847759835No Hit
GGGGATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACGTCTG892860.3174361077439713Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCCGCCCCTTGCCTCTCGGCGCCCAGATCGGAAGAGCACACGTCTGAAC891260.3168672640591939Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGAGATCGGAAGAGCAC851700.3028026039530725No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCAGATCGGAAGAGCA831650.2956742815282057No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTC823350.29272340491342286No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA820920.2918594735671672No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGT799640.2842938525596277No Hit
GGGGATGCGTGCATTTATCAGATCAAAACCAAGATCGGAAGAGCACACGT770970.2741008847830226No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTTAGATCGGAAGAGCACACGTCTG760200.2702718557298647Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGA759450.27000521025262525Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGCCCGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTCT702250.24966904852183303Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT690500.24549160271174894No Hit
TGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTG680490.2419327744088603Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGAGATCGGAAGAGCACA671980.2389072370604505No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTAGATCGGAAGAGCACACGTCTGA653990.23251130087973454Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACA652940.2321379972115994No Hit
ATGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCT642610.22846540017175523Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACAC637320.22658466073895994No Hit
GGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACGTC630470.22414929871350667No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTCT625000.22220456436617392Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACACG620970.2207717893351408No Hit
GGGATGCGTGCATTTATCAGATCAAAACCAGATCGGAAGAGCACACGTCT594400.21132542889480604Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGATGCGTGCATTTATCAGATCAAAACAGATCGGAAGAGCACACGTCT553170.1966670381926983Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGATGCGTGCATTTATCAGATCAAAACCAAGATCGGAAGAGCACACGTC551470.19606264177762228No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCTG535780.19048441839377386Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGT526370.18713890647267675No Hit
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACAC520430.18502707429294063No Hit
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCAGATCGGAAGAGCACACG518180.18422713786122238No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACGT510260.18141136162157426No Hit
GGCCCGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTCTG508310.1807180833807518Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT508250.18069675174257263Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCACA502960.17881601230977734No Hit
GGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGT479350.17042201268628077No Hit
GATGCGTGCATTTATCAGATCAAAACCAAAGATCGGAAGAGCACACGTCT474690.16876525545436655Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTAAACCATTCGTAGACGACCTGCTTCAGATCGGAAGAGCACACGTCTG443520.15768346942029673Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACAC442950.1574808188575948No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTC436750.15527654957908232No Hit
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGAGATCGGAAGAGCACACGT434070.15432373640708016No Hit
CCCGACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTC430200.15294784574452483No Hit
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACGT426240.15153995762470077No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG408770.14532889564153748No Hit
TGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG403600.14349081948510048No Hit
ATGCGTGCATTTATCAGATCAAAACCAGATCGGAAGAGCACACGTCTGAA400750.14247756667159073Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT398370.14163141169048432No Hit
CGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGTCT395420.14058260614667598Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCAGATCGGAAGAGCACACGTC388170.13800503320002835No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCAGATCGGAAGAGCACACG386260.13732597605132535No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCAC385250.1369668934753096No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG381980.13580431919454578TruSeq Adapter, Index 11 (100% over 49bp)
GCCCGCCCCTTGCCTCTCGGCGCCCCAGATCGGAAGAGCACACGTCTGAA379180.13480884274618535Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACA378500.13456708418015492No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT376990.13403023795264626Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACG376610.13389513757751162No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACAC376210.13375292665631727No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTC373460.13277522657310611No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACAC372530.13244458618132923No Hit
CGTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC371740.13216371961197038No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAGATCGGAAGAGCA371500.13207839305925378No Hit
GTGCATTTATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTCTGAA362970.12904574516478423Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACGTCTGA355870.1265215013135845Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGAGATCGGAAGAGCACAC351030.12480074916713284No Hit
GATGCGTGCATTTATCAGATCAAAACCAAGATCGGAAGAGCACACGTCTG347370.12349951923820454Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GATGCGTGCATTTATCAGATCAAAACCAGATCGGAAGAGCACACGTCTGA344910.12262492207285927Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACG344500.12247915587863507No Hit
GGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACAC336140.11950694762567313No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCAGATCGGAAGAGCACAC324710.11544327055254452No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC321020.11413137480452665No Hit
GAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA318550.11325322236615153No Hit
GGATGCGTGCATTTATCAGATCAAAACCAAAGATCGGAAGAGCACACGTC318130.11310390089889746No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGAGATCGGAAGAGCACACGT315060.11201243207873081No Hit
GTGCATTTATCAGATCAAAACCAACCCGGTAGATCGGAAGAGCACACGTC313690.11152535967364015No Hit
CCCCGCCCCTTGCCTCTCGGCGCCAGATCGGAAGAGCACACGTCTGAACT309820.1101494690110848Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGTCT305870.10874513616429059Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACAC299810.10659064070819615No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCT297970.10593647047070216Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC295140.10493032820325211No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTC293760.1044397005251316No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACGT286130.10172702720334934No Hit
TCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCAGATCGGAAGAGCACACG284970.10131461553188573No Hit
GGATGCGTGCATTTATCAGATCAAAACCAGATCGGAAGAGCACACGTCTG284870.10127906280158715Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTAAACCATTCGTAGACGACCTGCTTCAGATCGGAAGAGCACACGTCTGA284770.10124351007128857Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)