FastQCFastQC Report
Fri 7 Jul 2023
SRR7640017_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7640017_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29109319
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length85
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCT13600424.672187624863365Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC12510014.297596244006945No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC10251303.5216557281879393No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG9294883.193094280220022No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT8132042.7936208332458756No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG7501942.577160942858196No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA7149592.456117231735995No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTC5980012.05432837504718No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG4630611.5907654864753107TruSeq Adapter, Index 12 (100% over 49bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC3870981.3298078185889544No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG3625301.2454087297610776No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG3335241.1457636642066413No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT2906400.9984431446163341No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG2876100.9880341068782819No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACG2750590.9449173304260398No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGT2582680.887234771792497No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC2306390.7923201501209974No Hit
TGCGTGCATTTATCAGATCAAAACCAAAGATCGGAAGAGCACACGTCTGA2300400.79026238985529Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC2265170.778159736406063No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCT2237260.768571741578702Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC2077760.7137782920995164No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGTC2062900.7086733976840888No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACGT1930560.6632102935833023No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACG1927440.6621384718756217No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCAGATCGGAAGAGCACACGTCTGAA1839580.6319556977612564Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGTGGCGCAATGAAGGTGAAGGGCCAGATCGGAAGAGCACACGTCTGAA1709790.5873686017869398Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACAC1616840.5554372467456212No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCACA1548930.5321079479736369No Hit
GGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTG1495920.5138972849210247Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA1460180.5016194298465038Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGCGTGCATTTATCAGATCAAAACCAAAGATCGGAAGAGCACACGTCTG1282320.440518721856736Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC1256340.43159374494470315No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTCT1206280.4143965030580069Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTCTG1198340.41166885422499927Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT1171490.40244500395217075No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCAGATCGGAAGAGCA1139720.3915309732941537No Hit
TGCGTGCATTTATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTCT1109410.3811185002301153Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCAGATCGGAAGAGCACACGTCTGA1099340.3776591269620564Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTG1050240.3607916763700312Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCCGCCCCTTGCCTCTCGGCGCCCAGATCGGAAGAGCACACGTCTGAAC999260.3432783844926087Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACA964480.3313303207127587No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCAGATCGGAAGAGCACACGTCTG959870.32974663543314087Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCAC920020.3160568613783098No Hit
CGTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA918930.31568241084581883No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTTAGATCGGAAGAGCACACGTCTG889480.3055653758165899Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGT854470.2935382995390583No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACAC831300.2855786492291352No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTAGATCGGAAGAGCACACGTCTGA830250.2852179400005888Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA824470.28323232158059075No Hit
TGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTG793590.2726240349353415Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCAGATCGGAAGAGCACACGTC783090.26901694264987785No Hit
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACACG776970.2669145231463505No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT767100.2635238563980147No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACA744780.25585620879691484No Hit
GGGGATGCGTGCATTTATCAGATCAAAACCAGATCGGAAGAGCACACGTC736590.2530426768142532No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC709820.243846309149314No Hit
GGGGATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACGTCTG699750.24038693588125506Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAGATCGGAAGAGCA680210.23367430890430654No Hit
CCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGAGATCGGAAGAGCACA627130.21543959856979134No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCTG622270.21377003014051962Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA604830.20777882162066383No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG604830.20777882162066383No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTC589740.20259491470755467No Hit
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGAGATCGGAAGAGCACACGT587860.20194907342215734No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCTG553690.19021056452746282Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCAGATCGGAAGAGCACACG540500.1856793695517233No Hit
ATGCGTGCATTTATCAGATCAAAACCAAGATCGGAAGAGCACACGTCTGA533590.18330555929528958Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGCCCGTCACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTC527270.1811344332720391No Hit
TCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGAGATCGGAAGAGCAC512430.17603640950858382No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC512230.17596770298886072No Hit
GGGCCCGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTCT510880.17550393398072966Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT498340.17119603519409024Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGT492060.1690386504747844No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGT481240.16532162775776377No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTC480550.165084590264719No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTG467160.16048468876925634Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACGTC450750.1548473188259746No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACAC443430.15233266020410852No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGTAGATCGGAAGAGCACACGTCT438480.15063217384096136Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCT433710.14899352334556504Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACA433130.148794274438368No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACG432170.14846448314369706No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT430550.1479079603339398Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGATGCGTGCATTTATCAGATCAAAACAGATCGGAAGAGCACACGTCT429530.1475575570833519Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCGTGCATTTATCAGATCAAAACCAAGATCGGAAGAGCACACGTCTGAA425200.14607006093134642Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG422020.14497762726774885No Hit
TGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG419230.14401917131761138No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTC416180.14297139689183386No Hit
CGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGTCT392610.13487433354246453Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACGT391370.1344483531201812No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCAGATCGGAAGAGCACACG389530.13381625313872852No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACAC383560.1317653635249935No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACAC376190.12923352827319665No Hit
GGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGA370640.12732692235088014Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGAGATCGGAAGAGCACAC368690.12665703378357976No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCTGA355380.12208461489600633Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA351090.12061086004794548No Hit
CGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGCA350140.12028450407926067No Hit
TGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT330960.11369554883781377No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTG329530.11320429722179348Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCAGATCGGAAGAGCACAC327280.1124313488749084No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCAGATCGGAAGAGCACACGT320370.11005753861847473No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT312940.10750509141076094No Hit
GGGATGCGTGCATTTATCAGATCAAAACCAGATCGGAAGAGCACACGTCT311240.10692108599311444Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC311110.10687642675529441No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGTCT304330.10454727573668075Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCCGCCCCTTGCCTCTCGGCGCCAGATCGGAAGAGCACACGTCTGAACT295940.10166503723429601Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCGACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTC291610.10017754108229052No Hit
GCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTC291440.10011914054052587No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)