FastQCFastQC Report
Mon 24 Jul 2023
SRR7698526.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7698526.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269943816
Total Bases13.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC3628774413.44270246220421No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA97849833.6248220629732817No Hit
GGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTCT78106892.893449872546812Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGACGGACGAACCTCTGGTGTGCCAGTTAGATCGGAAGAGCACACGTCT69211062.563906112966855Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGACGGACGAACCTCTGGTGTGCCAGTTAGATCGGAAGAGCACACGTC56270152.0845133937055995No Hit
ATTCGATACGGGCTAGCTAGAGTGTGGTAGATCGGAAGAGCACACGTCTG49359661.8285160494285968Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGT46285331.7146282765744112No Hit
ACAAGCAGCGGAGCATGTGGCTTAATTCGAGATCGGAAGAGCACACGTCT28255051.0467011402105986Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCTGTGGGAGAGTAGGACGCCGCCGAACAGATCGGAAGAGCACACGTC24213690.8969899869830692No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAGCACACGTC21675510.8029637545021592No Hit
GAGGCGTTCGGGTGAACTATGGTTAAGGAACTAGATCGGAAGAGCACACG21026290.7789135647397086No Hit
AAACGGTGGGAACTAGGTGTTGGCGACAGATCGGAAGAGCACACGTCTGA19054050.7058524356046Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGCGTTCGGGTGAACTATGGTTAAGGAACTAGATCGGAAGAGCACACGT18679780.6919876986550416No Hit
ACCCTGTGGGAGAGTAGGACGCCGCCGAAAGATCGGAAGAGCACACGTCT18514640.6858701293605481Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGTGGGCAATCTGCCCTGCACTCTGGGACAGATCGGAAGAGCACACGTC14740860.5460714091705661No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCCAGATCGGAAGAGCACACGTC14000470.5186438499483907No Hit
GAGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAGCACACGT13662280.5061156874214151No Hit
CTTGGGCTGCACACGTGCTACAATGGCCGATACAATAGATCGGAAGAGCA13567810.5026160703010881No Hit
AACACGTGGGCAATCTGCCCTGCACTCTGGGAGATCGGAAGAGCACACGT11074810.41026351942805755No Hit
GTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTAGATCGGAAGAGCACAC10604610.3928450800295421No Hit
CACAAGCAGCGGAGCATGTGGCTTAATTCGAGATCGGAAGAGCACACGTC9449810.3500658077679394No Hit
GGACGGACGAACCTCTGGTGTGCCAGTTAGATCGGAAGAGCACACGTCTG9444040.34985205958561394Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACACGTGGGCAATCTGCCCTGCACTCTGGGACAGATCGGAAGAGCACAC9412950.3487003384437597No Hit
AACACCGGTGGCGAAGGCGGATCTCTGGGCCATTAGATCGGAAGAGCACA8321760.3082774824521262No Hit
ATTCACGGAGAGTTTGATCCTGGCTCAGGACAGATCGGAAGAGCACACGT8252360.3057065771049188No Hit
ACACCGGTGGCGAAGGCGGATCTCTGGGCCATTAGATCGGAAGAGCACAC7728810.2863118005266696No Hit
CTAATACCGGATAATACTCTCGCAGGCAGATCGGAAGAGCACACGTCTGA7711270.2856620356881967Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGCTCGTAGGCGGCTTGTCACGTCGGGAGATCGGAAGAGCACACGTCTG7698270.2851804539949157Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGTGATGGGGACTCACAGGAGACCGCCGGGGTAGATCGGAAGAGCACA7575560.2806346932578No Hit
ACCGGTGGCGAAGGCGGATCTCTGGGCCATTAGATCGGAAGAGCACACGT7426300.275105394524022No Hit
ACCGCTGGAGGTGACTGGTACTAATAGGCCGAGGGCTTAGATCGGAAGAG6934820.2568986429383513No Hit
CACGTCGTCGGTGCCGCAGCTAACGCATTAGATCGGAAGAGCACACGTCT6688580.24777674477269745Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACAC6347750.23515078411724016No Hit
ACCCTGTGGGAGAGTAGGACGTCGCCGAACAGATCGGAAGAGCACACGTC6239240.23113105876816975No Hit
ACCCTGTGGGAGAGTAGGACGTCGCCGAAAGATCGGAAGAGCACACGTCT6238640.23110883192078757Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGTGATGGGGACTCACAGGAGACCGCCGGGGTCAGATCGGAAGAGCAC6096170.22583106700988476No Hit
CGGGACGGACGAACCTCTGGTGTGCCAGTAGATCGGAAGAGCACACGTCT6067860.22478233026090141Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACACGTGGGCAATCTGCCCTGCACTCTGGGAAGATCGGAAGAGCACACG5854140.21686512722336265No Hit
ACGTGGGCAATCTGCCCTGCACTCTGGGCTAGATCGGAAGAGCACACGTC5504070.20389687311821955No Hit
GGGACGGACGAACCTCTGGTGTGCCAGTAGATCGGAAGAGCACACGTCTG5377760.19921775129681057Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCTGTGGGAGAGTAGGACGCCGCCGAACAGATCGGAAGAGCACACGTCT5182190.19197291039258332Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTGAGAACTTAAGCGCCAGTAAACGGCGGTGGTAAGATCGGAAGAGCA5149620.1907663630271864No Hit
GAGGCGTTCGGGTGAACTATGGTTAAGGAACTCAGATCGGAAGAGCACAC5087320.1884584753740015No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGGTGGTAGATCGGAAGAGCAC5063700.1875834784820557No Hit
GCGGGTGTAGTTTAATGGTAGAACATGAGCAGATCGGAAGAGCACACGTC4994130.1850062755280899No Hit
CGGGTGTAGTTTAATGGTAGAACATGAGCAGATCGGAAGAGCACACGTCT4809450.1781648519038495Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAAAGTCGGTCTCAGTTCGGATTGGGGTAGATCGGAAGAGCACACGTCT4642830.17199245638581326Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTGAGAACTTAAGCGCCAGTAAACGGCGGTAGATCGGAAGAGCACACG4608490.17072033982063883No Hit
GGAGGTGACTGGTACTAATAGGCCGAGGGCTTAGATCGGAAGAGCACACG4265800.15802547593829672No Hit
TTCACGGAGAGTTTGATCCTGGCTCAGGACAGATCGGAAGAGCACACGTC4157120.15399945298246803No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCCCAGATCGGAAGAGCACA3986210.14766813550564908No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCAGATCGGAAGAGCACACGTCTG3667760.13587123625754777Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGTGATGGGGACTCACAGGAGACCGCCGGGGAGATCGGAAGAGCACAC3624540.1342701623511168No Hit
ATTGGGCGTAAAGAGCTCGTAGGCGGCTTGTCAGATCGGAAGAGCACACG3451350.1278543828542455No Hit
AACACGTGGGCAATCTGCCCTGCACTCTGGGCTAGATCGGAAGAGCACAC3319420.12296706956235663No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACTCAGATCGGAAGAGCACACG3299880.12224321523260974No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCAGATCGGAAGAGCACACGTCT3195420.11837352110336916Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACACCGGTGGCGAAGGCGGATCTCTGGGCCAGATCGGAAGAGCACACGT3171790.11749815376396694No Hit
GGATGATCCTATGCTGTCGAGAAAAGCCCAGATCGGAAGAGCACACGTCT3169670.1174196189032165Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGTGATGGGGACTCACAGGAGACCGCCGGGAGATCGGAAGAGCACACG3164520.11722883846318599No Hit
GATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTCTG3088380.11440825153038513Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTTGGGCTGCACACGTGCTACAATGGCCGATACAGATCGGAAGAGCACA3079620.11408373955860504No Hit
ACAAGCAGCGGAGCATGTGGCTTAATTCAGATCGGAAGAGCACACGTCTG3077640.11401039096224379Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACGGACGAACCTCTGGTGTGCCAGTTAGATCGGAAGAGCACACGTCTGA3049970.11298536285046812Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGGTGGCGAAGGCGGATCTCTGGGCCAGATCGGAAGAGCACACGTCTG3042700.11271604754968716Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACGGTGGGAACTAGGTGTTGGCGAAGATCGGAAGAGCACACGTCTGAA3002760.11123648040894554Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTGGGCTGCACACGTGCTACAATGGCCGATACAATGAGATCGGAAGAGC2985930.1106130173398749No Hit
GGGACGGACGAACCTCTGGTGTGCCAGATCGGAAGAGCACACGTCTGAAC2981220.11043853658792464Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGCCGCAGAGACCAGCGAGAAGCGACTGTTTACTAGATCGGAAGAGCAC2899740.10742013071342223No Hit
CGGGACGGACGAACCTCTGGTGTGCCAGATCGGAAGAGCACACGTCTGAA2851440.10563086949915534Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTGGGCTGCACACGTGCTACAATGGCCGATACAAGATCGGAAGAGCACA2822650.10456435127226622No Hit
ACGTCGTCGGTGCCGCAGCTAACGCATTAGATCGGAAGAGCACACGTCTG2736650.10137850314748459Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACACCGGTGGCGAAGGCGGATCTCTGGGCCAGATCGGAAGAGCACACGTC2721720.10082542509512422No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)