FastQCFastQC Report
Mon 24 Jul 2023
SRR7698527.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7698527.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219153779
Total Bases11.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC3279768514.965603216908253No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAGCACACGTC51770392.3622859818447393No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA36898081.6836615899742253No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACTAGATCGGAAGAGCACACGT33787601.5417302021517958No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCCAGATCGGAAGAGCACACGTC28264181.2896962182888025No Hit
GAGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAGCACACGT17682850.8068694996128722No Hit
GAGGCGTTCGGGTGAACTATGGTTAAGGAACTAGATCGGAAGAGCACACG17041270.7775941659669031No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATAGATCGGAAGAGCA13758170.6277861172542226No Hit
GTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGT13205060.6025476749821411No Hit
GCGAAAGGTCCGGCGTAGAGGGTAAGACCCCCGTAGTCAGATCGGAAGAG8915330.4068070393620728No Hit
TGAATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC7261030.33132123174567757No Hit
TGCATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC6942470.31678531995562803No Hit
AGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATAGATCGGAAGAGCAC5842110.26657582756079234No Hit
GAATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAG5389740.24593415749403985No Hit
GAATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAACTAGATCGGAAGA5382060.24558371863621845No Hit
TGTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATAGATCGGAAGAG4634140.21145608445109224No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACTCAGATCGGAAGAGCACACG4104770.18730089979420342No Hit
TAGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC3752250.171215391179725No Hit
GACGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA3672370.1675704620179057No Hit
TCGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC3438280.1568889213632953No Hit
NGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC3423940.15623458630845694No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCCCAGATCGGAAGAGCACA3307770.15093374228331238No Hit
AATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAGC3168570.1445820379852998No Hit
AAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACAC2980110.13598259695079226No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCAAGATCGGAAGAGCAC2957560.13495363910653804No Hit
CTTGGGCTGCACACGTGCTACAATGGCCGATACAATGAGATCGGAAGAGC2948750.13455163828135494No Hit
AATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAACTAGATCGGAAGAG2864880.13072464518168314No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCAAGATCGGAAGAGCACACGTC2711940.1237459838645995No Hit
GAGGCGTTCGGGTGAACTATGGTTAAGGAACTCAGATCGGAAGAGCACAC2430960.11092484971477494No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCGAGATCGGAAGAGCACACGTC2391800.10913797658036277No Hit
TGGACGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC2384110.10878708142194528No Hit
TGGAAGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC2297420.10483141155416718No Hit
TGGATGCTCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC2211310.10090220712096414No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)