FastQCFastQC Report
Thu 13 Jul 2023
SRR7698528.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7698528.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences230159662
Total Bases11.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC2324427610.099196270109227No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA116886845.078511107650132No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAGCACACGTC45444391.9744723990774717No Hit
GTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGT38324131.665110630897607No Hit
GAGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAGCACACGT25879211.1244025028156324No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCCAGATCGGAAGAGCACACGTC17377760.7550306534600316No Hit
CTTGGGCTGCACACGTGCTACAATGGCCGATACAATGAGATCGGAAGAGC17208260.7476662005177954No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACTAGATCGGAAGAGCACACGT16019320.6960090165582534No Hit
GAATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAG14454790.628033160736915No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATAGATCGGAAGAGCA14132160.6140155002486926No Hit
AATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAGC14122250.6135849295781465No Hit
GCGAAAGGTCCGGCGTAGAGGGTAAGACCCCCGTAGTCAGATCGGAAGAG13074120.5680456725731549No Hit
GAGGCGTTCGGGTGAACTATGGTTAAGGAACTAGATCGGAAGAGCACACG12586990.5468807996424673No Hit
AGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATAGATCGGAAGAGCAC11659020.5065622663279719No Hit
AAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACAC9937420.43176201744682785No Hit
TAATACCGGATAATACTCTCGCAGGCATCTGTGAGAGATCGGAAGAGCAC9617850.41787730814446544No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCCCAGATCGGAAGAGCACA8960590.38932060996857043No Hit
AATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAACTAGATCGGAAGAG5121990.22254073348439313No Hit
CTTGGGCTGCACACGTGCTACAATGGCCGATACAATAGATCGGAAGAGCA4831360.20991341219470508No Hit
CTAATACCGGATAATACTCTCGCAGGCATCTGTGAGAGATCGGAAGAGCA4566270.19839575537784726No Hit
TGGGCGTAAAGAGCTCGTAGGCGGCTTGTCACGTCGGGAGATCGGAAGAG4471120.19426166866720546No Hit
ATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAGCA3934550.17094872167478242No Hit
TGTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATAGATCGGAAGAG3916560.17016709035660646No Hit
GACGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA3916140.17014884215462567No Hit
CGGGACGGACGAACCTCTGGTGTGCCAGTTAGATCGGAAGAGCACACGTC3547140.15411649327152732No Hit
GGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTCT3511460.15256626506516158Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATAGATCGGAAGAGC3482820.15132191148247343No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGGTGGTAGATCGGAAGAGCAC3132010.13607988353754186No Hit
TGGCGAACGGGTGAGTAACACGTGGGCAATCTGCCCTGAGATCGGAAGAG3043270.1322242991476065No Hit
CCGCTGGAGGTGACTGGTACTAATAGGCCGAAGATCGGAAGAGCACACGT3014090.13095648359094306No Hit
GTGGATGATCCTATGCTGTCGAGAAAAGCCCAGATCGGAAGAGCACACGT2911620.12650435678863658No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCAAGATCGGAAGAGCAC2640380.11471949415706041No Hit
ACCGCTGGAGGTGACTGGTACTAATAGGCCGAGGGCTTAGATCGGAAGAG2462550.10699311854220571No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)