FastQCFastQC Report
Thu 13 Jul 2023
SRR7698529.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7698529.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences266436355
Total Bases13.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC3520865513.214658712772136No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAGCACACGTC107119144.020440078457011No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACTAGATCGGAAGAGCACACGT56568242.123142691994867No Hit
GCGAAAGGTCCGGCGTAGAGGGTAAGACCCCCGTAGTCAGATCGGAAGAG38731531.453687879794032No Hit
GAGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAGCACACGT35731611.341093635663947No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA34442241.2927004649947262No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCCAGATCGGAAGAGCACACGTC30499841.1447326698340397No Hit
GAGGCGTTCGGGTGAACTATGGTTAAGGAACTAGATCGGAAGAGCACACG29412241.1039124146552748No Hit
GAATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAG14154850.5312657125939139No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATAGATCGGAAGAGCA13385410.5023867707543139No Hit
GAATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAACTAGATCGGAAGA11059730.41509838250114184No Hit
AGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATAGATCGGAAGAGCAC9665900.36278457570101497No Hit
AATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAGC9029550.33890082305021774No Hit
TAATACCGGATAATACTCTCGCAGGCATCTGTGAGAGATCGGAAGAGCAC7809940.2931259136914705No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACTCAGATCGGAAGAGCACACG7628260.2863070244299056No Hit
GGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTCT7426320.27872772842880245Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGT6126110.22992770637475504No Hit
AATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAACTAGATCGGAAGAG5753500.2159427530075616No Hit
TGAATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC5081170.1907085840443959No Hit
TGCATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC4911780.18435096817024088No Hit
AAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACAC4626460.17364221935854063No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCAAGATCGGAAGAGCAC4238790.15909202781279602No Hit
TGGATGCTCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC3933780.14764426573843498No Hit
GAGGCGTTCGGGTGAACTATGGTTAAGGAACTCAGATCGGAAGAGCACAC3928760.14745585301225128No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACCAGATCGGAAGAGCACACGT3740360.14038474591802608No Hit
TGTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATAGATCGGAAGAG3635440.13644684487595546No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCGAGATCGGAAGAGCACACGTC3303680.12399509068497802No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCAAGATCGGAAGAGCACACGTC3157500.11850860217630585No Hit
AGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCAAGATCGGAAGAGCACA3149520.11820909350002179No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCCCAGATCGGAAGAGCACA3063200.11496929538763583No Hit
TAGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC3045180.11429296125898435No Hit
CCGCTGGAGGTGACTGGTACTAATAGGCCGAAGATCGGAAGAGCACACGT3042570.11419500165433505No Hit
GGGACGGACGAACCTCTGGTGTGCCAGTTAGATCGGAAGAGCACACGTCT2988630.11217050315824954Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATAGATCGGAAGAGC2917740.10950983021817726No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAAGATCGGAAGAGCACACGTCTG2723240.10220977538894795Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAACTAGATCGGAAGAGC2699450.10131687922243195No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)