FastQCFastQC Report
Fri 8 Sept 2023
SRR7698530.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7698530.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences315653269
Total Bases16 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC4193499213.285144213095416No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA222617607.052599223992197No Hit
GTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGT88725922.810866501750058No Hit
CTAATACCGGATAATACTCTCGCAGGCATCTGTGAGAGATCGGAAGAGCA35506871.124869389519929No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCCAGATCGGAAGAGCACACGTC32221071.0207741583693213No Hit
CTTGGGCTGCACACGTGCTACAATGGCCGATACAATGAGATCGGAAGAGC21767370.6895974836237163No Hit
GGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTCT19993440.633398794295395Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCCCAGATCGGAAGAGCACA17647100.5590659667776163No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAGCACACGTC17279820.5474304148581461No Hit
TAATACCGGATAATACTCTCGCAGGCATCTGTGAGAGATCGGAAGAGCAC13255650.4199433778080087No Hit
AATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAGC8949690.28352914032390397No Hit
AAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACAC8231840.2607874148136891No Hit
TAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA7341070.23256752649059373No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGGTGGTAGATCGGAAGAGCAC7295090.2311108648774995No Hit
GCAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA6989690.22143569183184983No Hit
GTGGATGATCCTATGCTGTCGAGAAAAGCCCAGATCGGAAGAGCACACGT6913680.21902766988293096No Hit
TCGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC6509200.20621360965534624No Hit
TCTTGGGCTGCACACGTGCTACAATGGCCGATACAATGAGATCGGAAGAG6183590.1958981771229494No Hit
GAGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAGCACACGT5577510.17669736219332485No Hit
TTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGT5414050.171518895310411No Hit
TGCATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC5249730.1663131833429547No Hit
GACGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA5164640.1636175039897971No Hit
GCGGGTGTAGTTTAATGGTAGAACATGAGCAGATCGGAAGAGCACACGTC4848780.15361095468331742No Hit
GAATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAG4789490.15173262786643277No Hit
GCCGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA4583460.14520552929866853No Hit
TGAATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC4452240.14104843628278724No Hit
TAGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC4205650.13323638349520783No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGGTGGTAAGATCGGAAGAGCA4052320.12837883836393915No Hit
GCGAAAGGTCCGGCGTAGAGGGTAAGACCCCCGTAGTCAGATCGGAAGAG3849530.12195438406817195No Hit
CTCATACCGGATAATACTCTCGCAGGCATCTGTGAGAGATCGGAAGAGCA3785530.11992684289292121No Hit
CTTGGGCTGCACACGTGCTACAATGGCCGATACAATAGATCGGAAGAGCA3745320.11865297678890821No Hit
TGGATGATCCTATTCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC3643920.11544059123937032No Hit
CTAATACCGGATAATACTCTCGCAGGCATCTGTGAAGATCGGAAGAGCAC3542650.11223232413284463No Hit
GAATTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA3539550.11213411510716843No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCAGATCGGAAGAGCACACGTCT3462760.10970138250017618Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGCTGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGT3349610.10611675306299458No Hit
TGGGTGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC3209310.10167200264287457No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCGAGATCGGAAGAGCACACGTC3203300.10148160385438619No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATAGATCGGAAGAGCA3193530.10117208702185182No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)