FastQCFastQC Report
Fri 15 Sept 2023
SRR7698531.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7698531.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences308070582
Total Bases15.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC6021908719.547172147712566No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAGCACACGTC59147211.9199239867700189No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA55986711.8173338601996085No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCCAGATCGGAAGAGCACACGTC51516411.6722275027220874No Hit
GGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTCT38093481.23651793536067Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGT15358400.49853510517924104No Hit
TGCATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC13855460.4497495317485394No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACTAGATCGGAAGAGCACACGT13276000.43094020577401315No Hit
TCGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC13171640.42755267038123107No Hit
TAGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC11128790.36124156768723864No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCAGATCGGAAGAGCACACGTCT11026410.3579183032802528Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAGCACACGT9426730.3059925403718035No Hit
TGAATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC9361620.3038790636621058No Hit
TAATACCGGATAATACTCTCGCAGGCATCTGTGAGAGATCGGAAGAGCAC8821320.2863408749622189No Hit
TGGACGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC8239030.26743968692213527No Hit
TGGAAGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC6758690.21938771161213957No Hit
CTAATACCGGATAATACTCTCGCAGGCATCTGTGAGAGATCGGAAGAGCA6647490.21577814917751542No Hit
AATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAGC6617690.2148108383811863No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAAAGATCGGAAGAGCACACGTCT5456090.17710519338065198Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATAGATCGGAAGAGCA5347510.17358067639187957No Hit
AAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACAC5203580.16890869508598522No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCAAGATCGGAAGAGCACACGTC5027780.16320221058952003No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCCCAGATCGGAAGAGCACA4924020.1598341512530398No Hit
TGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTCT4706700.15277992366048115Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC4685890.15210442910774258No Hit
GGGACGGACGAACCTCTGGTGTGCCAGTTAGATCGGAAGAGCACACGTCT4635610.15047233558964096Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGGCGTTCGGGTGAACTATGGTTAAGGAACTAGATCGGAAGAGCACACG4619080.1499357702385228No Hit
TAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA4571830.1484020308047459No Hit
TGGGTGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC4221090.137016977492515No Hit
GCAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA4179440.13566501458422278No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCGAGATCGGAAGAGCACACGTC3859450.12527810915746576No Hit
AGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC3756370.12193212268479436No Hit
TACGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA3610330.11719165058090486No Hit
TGGATGATCCTATGCTGTCGAGAAAGGCCTAGATCGGAAGAGCACACGTC3608520.11713289781106072No Hit
TGGTTGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC3560200.11556442607687871No Hit
TGCTGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTCT3474150.11277123500224373Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATAGATCGGAAGAGCAC3402220.11043638045258083No Hit
CGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC3352170.1088117527560616No Hit
GGATGATCCTATGCTGTCGAGAAAAGCCCAGATCGGAAGAGCACACGTCT3250030.10549627877159656Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA3097050.10053053361648143No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)