FastQCFastQC Report
Fri 8 Sept 2023
SRR7698532.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7698532.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences353167764
Total Bases18 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC300369338.50500415434292No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA217567856.160467408911081No Hit
GTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGT132682903.756936887365518No Hit
GGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTCT71774522.0323066631868474Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGATGATCCTATGCTGTCGAGAAAAGCCCAGATCGGAAGAGCACACGTC32686610.9255264305493069No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCCCAGATCGGAAGAGCACA24142790.6836068424410332No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAGCACACGTC20053680.5678230587319402No Hit
GCGGGTGTAGTTTAATGGTAGAACATGAGCAGATCGGAAGAGCACACGTC17934640.5078221125527188No Hit
CTAATACCGGATAATACTCTCGCAGGCATCTGTGAGAGATCGGAAGAGCA16635860.4710469554633531No Hit
CTGGAAACGGGGTCTAATACCGGATAATACTCTAGATCGGAAGAGCACAC14806890.41925938631250614No Hit
GTGGATGATCCTATGCTGTCGAGAAAAGCCCAGATCGGAAGAGCACACGT14736860.4172764760036253No Hit
TAATACCGGATAATACTCTCGCAGGCATCTGTGAGAGATCGGAAGAGCAC10905610.3087940381784109No Hit
AAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACAC9838090.27856704384831676No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACTAGATCGGAAGAGCACACGT8847530.25051918385167227No Hit
CGGGTGTAGTTTAATGGTAGAACATGAGCAGATCGGAAGAGCACACGTCT8762210.24810333482191768Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGATGATCCTATGCTGTCGAGAAAAGCCCAGATCGGAAGAGCACACGTCT7989980.2262375226296135Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCTCATCGTCTAGCGGCCTAGGACGCCGCAGATCGGAAGAGCACACGT7723770.21869974520098046No Hit
GGGACGGACGAACCTCTGGTGTGCCAGTTAGATCGGAAGAGCACACGTCT6883120.1948966100994427Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTGAGAACTTAAGCGCCAGTAAACGGCGGTGGTAGATCGGAAGAGCAC6314220.17878811838557268No Hit
ACCGCTGGAGGTGACTGGTACTAATAGGCCGAGGGCTTAGATCGGAAGAG5866290.1661049109793611No Hit
GAGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAGCACACGT5351740.1515353479430246No Hit
AGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATAGATCGGAAGAGCAC5143910.14565060926681858No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATAGATCGGAAGAGCA4856280.13750632121679146No Hit
TGGCTGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC4633320.13119317424452137No Hit
AATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAGC4436980.1256337766999595No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCAGATCGGAAGAGCACACGTCT4283110.12127692379081348Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGT4089940.11580728528779315No Hit
ATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAGCA3938870.11152971481281626No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)