FastQCFastQC Report
Mon 24 Jul 2023
SRR7698533.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7698533.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314771387
Total Bases16 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC3358693110.670261779543514No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA162541655.163800037517388No Hit
GTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGT120789593.8373751550677No Hit
GGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTCT59843011.9011578711250525Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGATGATCCTATGCTGTCGAGAAAAGCCCAGATCGGAAGAGCACACGTC44955191.4281854023790288No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCCCAGATCGGAAGAGCACA22281220.7078540464670634No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACAGATCGGAAGAGCACACGTC21833340.693625307182066No Hit
GGGACGGACGAACCTCTGGTGTGCCAGTTAGATCGGAAGAGCACACGTCT16405700.5211941325530963Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGATGATCCTATGCTGTCGAGAAAAGCCCAGATCGGAAGAGCACACGT16398990.5209809619703458No Hit
GCGGGTGTAGTTTAATGGTAGAACATGAGCAGATCGGAAGAGCACACGTC10981230.34886366593415935No Hit
CTAATACCGGATAATACTCTCGCAGGCATCTGTGAGAGATCGGAAGAGCA10469680.33261218879465687No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACTAGATCGGAAGAGCACACGT10042790.3190502826738823No Hit
TTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGT7983830.2536390005486744No Hit
AGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATAGATCGGAAGAGCAC5077380.16130373374756582No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCAGATCGGAAGAGCACACGTCT4551060.14458302717330532Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGATGATCCTATGCTGTCGAGAAAAGCCAAGATCGGAAGAGCACACGTC4268810.1356162019897952No Hit
GCAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA4242950.1347946533653645No Hit
TAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA3963650.12592154699245264No Hit
GCCGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA3756390.1193370857434383No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCTCACGTC3511960.11157176748088606No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)