FastQCFastQC Report
Fri 7 Jul 2023
SRR7777909.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7777909.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63960111
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length40
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCA1002477715.673482805556732TruSeq Adapter, Index 4 (100% over 39bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGA18747542.9311299975698915No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAA9621161.5042437934480757No Hit
CAGGAAGAAACCATGGTGCTCTCTGGGGAAGAGATCGGAA9558341.4944220468910692No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAA8595411.3438704007252271No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACA6810311.0647745748909037No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGAGATCGGAAG6753061.055823683608054No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAA6607501.033065749369947No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAG4907380.7672563294957383No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGA4706080.7357835886182249No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCG4695880.7341888446691407No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCG4652330.7273799133963355No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCAC4061040.6349332320577117No Hit
GTGCACCTGACTGATGCTGAGAAGGCTGCAGATCGGAAGA3776210.5904007890167671No Hit
CGACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGA3474710.5432620340511918No Hit
GAATACAAGCTTGGGCTGCAGGTCGACAGATCGGAAGAGC3467950.5422051253163085No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAA3298020.5156370038194587No Hit
CAGGAAGAAACCATGGTGCTCTCTGGGGAAAGATCGGAAG3291900.5146801574500082No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGG3059460.4783387571044084No Hit
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGGAGATCG2913970.455591767187521No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGC2774860.4338422739760411No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAA2675030.418234108442995No Hit
GAAACAGACATCATGGTGCACCTGACTGATAGATCGGAAG2578550.40314970685401097No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAT2462380.3849868240535105TruSeq Adapter, Index 4 (100% over 40bp)
GACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAG2428730.37972573249599273No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGA2196810.343465632822307No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGG2160640.33781054570089786No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAA2142120.3349149910011882TruSeq Adapter, Index 4 (100% over 40bp)
CGACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAG2116180.33085933825224284No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACG2039450.3188627987215344No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGG2026560.31684748014274083No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAG2015660.31514329298146465No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGG2004690.31342816149896924No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACAGA1980830.30969771143768027No Hit
GAAACAGACATCATGGTGCACCTGACTGAAGATCGGAAGA1971000.30816081604361195No Hit
ACCATGGTGCTCTCTGGGGAAGACAAAAGCAGATCGGAAG1943060.3037924684026893No Hit
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGC1839430.2875901825748864No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAG1805290.2822524807688342No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGA1777760.2779482355807669No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGG1672930.26155833281777763No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCA1657330.2591193126603548No Hit
CAGGAAGAAACCATGGTGCTCTCTGGGGAAGATCGGAAGA1645090.25720561992145385No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAA1633270.2553575931098681No Hit
GACTGATGCTGAGAAGGCTGCTGTCTCTGGCAGATCGGAA1628370.2545914906245238No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATC1582500.2474198332770248No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATC1574540.24617530760695522No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGA1568970.2453044523327985No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCAGATCGGAAGAG1345430.2103545442564976No Hit
ACCATGGTGCTCTCTGGGGAAGACAAAAGAGATCGGAAGA1343280.21001839724762206No Hit
ACAGACATCATGGTGCACCTGACTGATGCTAGATCGGAAG1308700.20461190256533482No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCAGATCGG1272210.1989067842612093No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAG1269080.19841741675526484No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGG1234700.19304219156217536No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAG1233980.19292962140106354No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAA1208110.1888849129733374No Hit
CTCTCTGGGGAAGACAAAAGCAACATCAAGAGATCGGAAG1152480.18018730455298929No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGA1130920.17681645361747417No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGG1130730.17678674760273633No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAG1084990.1696354154232159No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAG1066890.16680552665082146No Hit
GACGTACGCAGTTTTATCCGGTAAAGCGAAGATCGGAAGA1031810.16132085824553993No Hit
AACCATGGTGCTCTCTGGGGAAGACAAAAGCAGATCGGAA975320.15248879102164162No Hit
ACTGATGCTGAGAAGGCTGCTGTCTCTGGCAGATCGGAAG944090.14760606028341633No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCG941660.14722613598966394No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCG933460.14594408693255706No Hit
AGAAACAGACATCATGGTGCACCTGACTGATAGATCGGAA929370.14530462587846354No Hit
AGAAACAGACATCATGGTGCACCTGACTGAAGATCGGAAG914870.14303758791162824No Hit
TCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACAC904290.1413834319330684No Hit
CGACGTACGCAGTTTTATCCGGTAAAGCGAAGATCGGAAG876070.13697130700726895No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAA863490.13500445613673184No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACAGAT845110.1321307900794606No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCG840440.13140064750669367No Hit
AAAAGCAACATCAAGGCTGCCTGGGGGAAGAGATCGGAAG839090.131189578454609No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGAAGA821340.1284144112883106No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCAGATCGGAAGA816420.1276451818540465No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGG814630.12736531992572683No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGA814300.1273137252685506No Hit
GGTGCACCTGACTGATGCTGAGAAGGCTGCAGATCGGAAG812540.12703855376361056No Hit
GACTGATGCTGAGAAGGCTGCTGTCTCTGGCCAGATCGGA793130.12400384983697105No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAA778390.12169928848309848No Hit
ATGGTGCACCTGACTGATGCTGAGAAGGCTAGATCGGAAG757360.11841130169395736No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAA751260.11745758227342663No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGA747900.1169322548549048No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGG747330.11684313681069128No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAGATC745040.11648510115937728No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAG732480.11452137723775996No Hit
CACCTGACTGATGCTGAGAAGGCTGCTGTCAGATCGGAAG723190.11306890946452548No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATC715940.11193539048110783TruSeq Adapter, Index 4 (100% over 40bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCG688550.10765303393547895No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCG670690.10486066855012181No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGG660780.10331126536037437No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGG646170.10102702917448032No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGAA644900.10082846791807476No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATC643810.10065804920194714No Hit
GTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACA643800.10065648572748725No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAG641970.10037036990132803No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)