FastQCFastQC Report
Fri 7 Jul 2023
SRR7777911.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7777911.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58067739
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length40
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGA53363329.189839473515578TruSeq Adapter, Index 8 (100% over 39bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGA16635142.864781768065741No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAA15710812.7056004367588686No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAA13450462.316339542684794No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAG7412411.2765108694864113No Hit
CGACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGA7296351.2565238677538315No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAA7286801.2548792368168493No Hit
GACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAG7205771.2409248446887178No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACA5866091.0102149835728924No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCG4615670.7948768248062836No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGAGATCGGAAG4369440.7524729006583156No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAA3817710.6574580077932773No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGG3339220.5750559704072514No Hit
GAAACAGACATCATGGTGCACCTGACTGAAGATCGGAAGA3312570.5704665029234218No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAG3161080.5443780065209702No Hit
GTGCACCTGACTGATGCTGAGAAGGCTGCAGATCGGAAGA3065490.5279161980114294No Hit
CAGGAAGAAACCATGGTGCTCTCTGGGGAAGAGATCGGAA3053040.5257721503501281No Hit
GAATACAAGCTTGGGCTGCAGGTCGACAGATCGGAAGAGC3042140.5238950323173422No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGG2870100.494267565678767No Hit
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGC2762540.4757443715864329No Hit
CAGGAAGAAACCATGGTGCTCTCTGGGGAAAGATCGGAAG2725740.4694069455674863No Hit
CAGGAAGAAACCATGGTGCTCTCTGGGGAAGATCGGAAGA2679130.4613801133190324No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGG2584430.4450715740800585No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGA2514180.43297363446508574No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAG2484260.42782103157142043No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCG2451400.42216212344689363No Hit
CGACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAG2371760.40844710692110814No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGG2253880.388146678140852No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGA2246790.38692569035622343No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCAC2244660.3865588773828442No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGA2236250.38511056888231865No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGAAGA2149210.3701211786462015No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGC2082160.35857431955461533No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGA1997580.34400857247085165No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGC1984960.3418352486567455No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAG1940240.33413389834241697No Hit
GAAACAGACATCATGGTGCACCTGACTGATAGATCGGAAG1808860.31150859860412333No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAG1761790.3034025485304327No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAA1701360.2929957372716027No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAA1658900.285683587576916TruSeq Adapter, Index 8 (100% over 40bp)
GCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGA1654670.28495512801006423No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAA1627090.2802055027491255No Hit
GACGTACGCAGTTTTATCCGGTAAAGCGAAGATCGGAAGA1586310.2731826703292167No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGG1529350.26337343701293414No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAAT1468520.25289774068868087TruSeq Adapter, Index 8 (100% over 40bp)
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAG1348030.2321478368565375No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGC1341880.23108872897565375No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCG1287540.221730692837894No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAG1268560.2184620964835569No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGG1266190.21805395247092366No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCG1262560.2174288205022069No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACG1233930.2124983719445319No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGG1202060.20700995435692787No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCA1194660.2057355806465962No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCG1182570.20365352954417598No Hit
ACCATGGTGCTCTCTGGGGAAGACAAAAGAGATCGGAAGA1169050.20132521433286732No Hit
GAAACAGACATCATGGTGCACCTGACTGAGATCGGAAGAG1135830.1956043096494596No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCA1135450.1955388688373074No Hit
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGGAGATCG1117110.1923804885876476No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCAGATCGGAAGAG1091130.18790640358840216No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCG1028470.1771155580898371No Hit
ACGCCGGGTTAAGGCGCCCGATGCCGACGCTCAGATCGGA1005590.1731753323476225No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCG999830.17218338740552652No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGAA951120.16379490856360018No Hit
TCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGA923920.15911072411481356No Hit
CGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAG919930.1584235955872158No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAG907590.156298491318906No Hit
ACAGACATCATGGTGCACCTGACTGATGCTAGATCGGAAG900330.15504822738147253No Hit
AGAAACAGACATCATGGTGCACCTGACTGAAGATCGGAAG874150.15053969984951543No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGG870420.14989734661444284No Hit
GAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCAGAT864590.14889334678589777No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGG860900.14825788205736753No Hit
CGACGTACGCAGTTTTATCCGGTAAAGCGAAGATCGGAAG860380.1481683314723172No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGA849690.1463273780988786No Hit
TCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAG844330.14540431822220595No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATC830960.14310183491043107No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAA825550.1421701644005805No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGA793240.13660597324101081No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGA791490.13630460107978373No Hit
CGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGA789720.1359997846652855No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGG788280.13575179842976148No Hit
TCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACAC780390.13439304051428624No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCAGATCGG779310.13420705083764325No Hit
GTGCACCTGACTGATGCTGAGAAGGCTGAGATCGGAAGAG767510.13217494140765493No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATC739100.12728237963596276No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCG719780.1239552309760158No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGA705460.12148914563386047No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAG692590.11927276865386476No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATC683840.11776590784772936No Hit
ACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGC673540.11599211741307855No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGA665430.11459547271162047No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCAGATCGGAAGA662310.11405816920131848No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGA659480.11357080736344839No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAAGAAGATCGGAAG623950.1074520914272209No Hit
GTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCAC617520.10634476400054081No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAA596770.10277135123170544No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAG595860.10261463770786736No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)