FastQCFastQC Report
Fri 7 Jul 2023
SRR7777913.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7777913.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72824156
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length40
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGA22544023.0956788568891893No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTA22031623.0253175883013323TruSeq Adapter, Index 12 (100% over 39bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAA11398911.5652649651030626No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCG8964191.2309363393102695No Hit
CAGGAAGAAACCATGGTGCTCTCTGGGGAAGAGATCGGAA8394601.1527219072748334No Hit
CAGGAAGAAACCATGGTGCTCTCTGGGGAAAGATCGGAAG6299820.865072847531525No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGAGATCGGAAG5670210.7786166447298064No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCG3914550.5375345510355108No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACG3418540.46942390928636374No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGG3404030.4674314385462977No Hit
GAATACAAGCTTGGGCTGCAGGTCGACAGATCGGAAGAGC3330590.4573468726503332No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGG3300730.453246584828254No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGC2981800.4094520504981891No Hit
CAGGAAGAAACCATGGTGCTCTCTGGGGAAGATCGGAAGA2959500.4063898797536356No Hit
GTGCACCTGACTGATGCTGAGAAGGCTGCAGATCGGAAGA2870630.394186511409758No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGA2816550.38676040406153145No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAA2664660.36590331373013096No Hit
GAAACAGACATCATGGTGCACCTGACTGATAGATCGGAAG2528580.3472172063346673No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAG2510990.3448017990074612No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACA2439550.3349918672589903No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATC2080400.2856744402228294No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGA2053200.2819394158169166No Hit
ACAGACATCATGGTGCACCTGACTGATGCTAGATCGGAAG1811140.24870044494576773No Hit
GGTGCACCTGACTGATGCTGAGAAGGCTGCAGATCGGAAG1797850.24687550103567285No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATC1793590.2462905303015115No Hit
GAAACAGACATCATGGTGCACCTGACTGAAGATCGGAAGA1745210.2396471302736416No Hit
GACTGATGCTGAGAAGGCTGCTGTCTCTGGCAGATCGGAA1687260.23168960585001494No Hit
AGAAACAGACATCATGGTGCACCTGACTGATAGATCGGAA1644280.22578771802037773No Hit
CTCTCTGGGGAAGACAAAAGCAACATCAAGAGATCGGAAG1623940.22299468874036796No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAA1559190.21410340821526305No Hit
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCA1443150.19816913497768515No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAA1359460.186677069075816No Hit
ACTGATGCTGAGAAGGCTGCTGTCTCTGGCAGATCGGAAG1273430.17486368122137935No Hit
AGAAACAGACATCATGGTGCACCTGACTGAAGATCGGAAG1266910.17396837390055025No Hit
TGCCAAAGTGAAGGCCCATGGCAAGAAAGTGAGATCGGAA1256950.1726006958460322No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCAGATCGGAAGAG1251230.17181524218420052No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAA1219390.1674430665561026No Hit
GAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG1186170.16288139336623414No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGAAGA1171780.1609054006750178No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACAGA1137270.15616658846001594No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGC1128280.15493210796703227No Hit
AAAAGCAACATCAAGGCTGCCTGGGGGAAGAGATCGGAAG1076420.14781084452252355No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCAC1074470.1475430762287173No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGG1052500.14452622011850025No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGA1052330.14450287621596328No Hit
ACAGACATCATGGTGCACCTGACTGATGCAGATCGGAAGA1027760.14112899571400456No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAA1013660.1391928249741748No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGG1012310.1390074469246166No Hit
GTCTAGCGGTTAGGATTCCTGGTAGATCGGAAGAGCACAC1000130.13733492496638067No Hit
CGACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGA998420.13710011277027365No Hit
CTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACA978860.13441419080778635No Hit
ACCATGGTGCTCTCTGGGGAAGACAAAAGCAGATCGGAAG974760.13385119080542449No Hit
CTCTCTGGGGAAGACAAAAGCAACATCAAGGAGATCGGAA966240.13268124933710182No Hit
AGTGAGCTCCACTGTGACAAGCTGCATGTGAGATCGGAAG955800.1312476590871853No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAA941060.12922360542015757No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAG914460.12557097125849287No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCA875880.12027327855334156No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCAGATC861330.11827531513032571No Hit
CTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT843130.11577614438813408No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCG801310.11003354436404317No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAG794340.10907644436002802No Hit
GACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAG779570.10704827118078787No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAA769590.10567784678479487No Hit
GAAGAAACCATGGTGCTCTCTGGGGAAGAAGATCGGAAGA766720.10528374678314158No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGA764460.10497341019647381No Hit
CTGGTGTAGTGGTATCATGCAAGAGATCGGAAGAGCACAC763430.10483197361051463No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGAGATCGGAAGAG762920.10476194190290376No Hit
GTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACAC755390.1037279443375904No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCG742070.10189888091528311No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGG736290.10110518822902664No Hit
CCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACA731160.1004007516407056No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGG729330.10014946139574896No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)