FastQCFastQC Report
Mon 10 Jul 2023
SRR7777915.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7777915.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67065320
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length40
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACA15916562.3732921873779174No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGA8437271.258067507916163No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCAC8321531.2408097061193475No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAA6101370.9097652855454951No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGC5733530.8549172657343616No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGC5551770.8278153298903218No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAG4885100.7284092583171153No Hit
CAGGAAGAAACCATGGTGCTCTCTGGGGAAGAGATCGGAA4801810.7159900228612941No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCA4778880.7125709681248072No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCG4749260.7081543784477581No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAG4610150.687411914235256No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAG4318500.6439244605110361No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCG3960820.5905913816559736No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGG3742270.5580037491806495No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTAGATCGGAAG3725190.5554569783607981No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTG3708840.5530190566450738TruSeq Adapter, Index 5 (100% over 39bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGG3610240.5383169721698189No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGG3501670.5221282773272387No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGA3318340.49479224135514455No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAA3212000.4789360581594183No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAA3162360.4715343190787728No Hit
ACAGACATCATGGTGCACCTGACTGATGCTAGATCGGAAG3019460.45022673417498044No Hit
CAGGAAGAAACCATGGTGCTCTCTGGGGAAAGATCGGAAG2885390.4302357761060411No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGG2721080.40573578117572534No Hit
GACTGATGCTGAGAAGGCTGCTGTCTCTGGCAGATCGGAA2701480.4028132572840926No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAG2694830.4018216866780029No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGA2560640.38181283560564533No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAG2553980.38081977391593747No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGG2539190.37861446124464926No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGA2517750.37541757796727127No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCG2347040.34996328952132044No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCA2276550.33945264109676954No Hit
CGACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGA2195270.32733311344820243No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGA2145290.31988067752453875No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGA2141930.3193796734288303No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGA2133400.31810777910252275No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAA2023250.30168349304826997No Hit
GTGCACCTGACTGATGCTGAGAAGGCTGCAGATCGGAAGA1958310.292000396031809No Hit
GACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAG1874100.2794439808831152No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGA1859500.2772669988005723No Hit
CAGGAAGAAACCATGGTGCTCTCTGGGGAAGATCGGAAGA1855530.2766750386041549No Hit
AGAAACAGACATCATGGTGCACCTGACTGATAGATCGGAA1736460.25892070596248556No Hit
TAAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAG1733610.2584957471313042No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGG1703000.25393154017605524No Hit
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGC1685200.2512774113356948No Hit
GAAACAGACATCATGGTGCACCTGACTGATAGATCGGAAG1681520.25072869256420455No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGA1654470.24669531137702766No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGA1609670.24001525676758123No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAG1600080.23858530757774657No Hit
CCGACGTACGCAGTTTTATCCGGTAAAAGATCGGAAGAGC1556550.23209462058780903No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGA1544360.2302769896572476No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATC1543520.2301517386333205No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCG1506870.22468691717269074No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAA1487680.2218255277094033No Hit
ACTGATGCTGAGAAGGCTGCTGTCTCTGGCAGATCGGAAG1465520.2185212864115164No Hit
GACTGATGCTGAGAAGGCTGCTGTCTCTGGCCAGATCGGA1436950.21426126051437613No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGG1418490.21150872015521585No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGG1346690.20080273977668336No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGTTAGATCGGAA1318410.19658595530447032No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCG1308990.19518135453614477No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGAGATCGGAAGA1287090.19191588141233054No Hit
GGTGCACCTGACTGATGCTGAGAAGGCTGCAGATCGGAAG1219940.18190325491625178No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATC1209240.1803077954447992No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTAGATCGGAAGA1197380.1785393702736377No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAGATCGGAAG1192040.17774313162152958No Hit
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGGAGATCG1177820.17562281071647762No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGTCGGAG1145350.17078126220824713No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATC1123600.1675381553387056No Hit
CTCCATCTAAGGCTAAATACCGGCACGAGACCGAAGATCG1107560.16514645721514487No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACAGA1088470.16229997858803924No Hit
AACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAG1076150.16046296357044146No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAG1041070.15523224223786602No Hit
AGAAACAGACATCATGGTGCACCTGACTGATGAGATCGGA1031010.1537322121179769No Hit
GTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGC1022680.15249013946403298No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAA999300.1490039859647281No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAA991680.14786778024767497No Hit
CTGGTTGTCTACCCTTGGACCCAGCGGTACTAGATCGGAA960320.14319174202106244No Hit
GAAACAGACATCATGGTGCACCTGACTGAAGATCGGAAGA954570.14233436894060894No Hit
AGAAACAGACATCATGGTGCACCTGACTGAAGATCGGAAG940820.14028412896561143No Hit
CACCTGACTGATGCTGAGAAGGCTGCTGTCAGATCGGAAG935990.13956393557803048No Hit
AACAGACATCATGGTGCACCTGACTGATGCTAGATCGGAA935000.13941631829983067No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGAGATCGGAAGAG929720.13862902614943162No Hit
CGACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAG928120.13839045277052284No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGA901940.1344867958581276No Hit
TAAACGATGCCGACTGGCGATGCGGCGGCAGATCGGAAGA895270.1334922430848015No Hit
CGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAGAT887610.13235007303327562No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGAGATCGGAAG873380.13022826104460547No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAG856570.12772174948244489No Hit
AAACGATGCCGACTGGCGATGCGGCGGCAGATCGGAAGAG849770.12670781262208247No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCG846280.12618742443933764No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCAC845640.12609199508777413No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCG839470.12517199649535707No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGG821550.1224999746515785No Hit
CTGGTTGTCTACCCTTGGACCCAGCGGTACAGATCGGAAG813800.12134438484748898No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGA807050.12033790340521748No Hit
GAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCAGAT800200.11931651112676417No Hit
ATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGC792090.11810724231242019No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGTAGATCGGAAG784780.1170172601875306No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGATCGGAAGAG777830.11598095707289549No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCAGATC774220.11544267588673252No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCG759190.11320157720860796No Hit
AGTGAGCTCCACTGTGACAAGCTGCATGTGAGATCGGAAG745010.11108722063802871No Hit
GGACCAAGGAGTCTAACGCGTGCGCGAGTCAGGGAGATCG743730.11089636193490167No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGA742640.11073383382052006No Hit
GAAACAGACATCATGGTGCACCTGACTGATGAGATCGGAA720230.10739231543217866No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATC707110.10543601372512648No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAA704420.10503491223183606No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCG692580.10326946922791094No Hit
ATCTAAGGCTAAATACCGGCACGAGACCGAAGATCGGAAG682280.10173365310118554No Hit
ACAGACATCATGGTGCACCTGACTGATGCTGAGATCGGAA682030.10169637601073103No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCG681110.10155919631785848No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAA671950.10019336372360559No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)