FastQCFastQC Report
Thu 28 Sept 2023
SRR7808449.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7808449.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103061695
Total Bases5.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA2229921221.63676038900777No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC1251323412.14149835203079No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAG81722547.92947758136522No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA18170161.7630371788470975No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAG14495911.4065274202990743No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG14477221.4047139434297096No Hit
CTTTTGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA11622961.1277672077875296No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC9410380.9130822077009311No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCA7608480.7382451841103526No Hit
GTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC7264450.7048642077932058No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGA7245040.7029808698566427No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA6376230.6186808784776924No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA5900440.5725153268631958No Hit
TTTGTTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC5839550.5666072152219115No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC5798010.5625766197615903No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA5230070.5074698218382688No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCAGATCGGAAG4739330.4598536827868006No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG4674390.45355260264252395No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCAC4224670.40991660383617795No Hit
CGGCGGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCGCCTCAGA4196450.40717843811903154No Hit
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAGG3513230.34088610710312883No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC3452120.3349566490246449No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCAGATCGGAAG3426240.33244553177589403No Hit
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAGA3071790.2980535105695671No Hit
CTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC3057950.2967106256111934No Hit
ATTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC2920680.28339141909125404No Hit
CTTTAGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA2851120.27664206376578615No Hit
GCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC2832140.27480044841102214TruSeq Adapter, Index 6 (100% over 35bp)
CGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCGCCTC2491540.2417522824556689No Hit
ATTGGTCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAG2407350.23358338905642878No Hit
TTTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAG2197710.21324217499042686No Hit
CCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTAG1973470.19148433372845267No Hit
CCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAGA1730280.16788778798951445No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC1632980.1584468409916992No Hit
CGGCGGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCGCCTCAGC1615190.156720690456333No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAA1593600.15462582873297395No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGAGATCGGAAG1440980.13981722307206376No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCAC1439670.13969011474146625No Hit
CTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTAGA1415750.1373691748423117No Hit
ATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAAC1287230.12489897434735572Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAGGA1256800.1219463739656135No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGA1171500.1136697780877755No Hit
TTTGGTGACTCTAGATAAACTCGGGCCGATCGCACAGATCGGAAGAGCAC1165120.11305073140898761No Hit
CTTTGGTGACTCTAGATAGCCTCGGGCCGATCGCACAGATCGGAAGAGCA1149060.1114924414934181No Hit
CTTTGGTGACTCTAGATACCCTCGGGCCGATCGCACAGATCGGAAGAGCA1148930.11147982768961835No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAA1133100.1099438545038484No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAGATCGGAAGAGCA1109010.10760641963049414No Hit
GTTGTTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC1100680.10679816589470996No Hit
TTTGATGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC1092240.10597923894032597No Hit
GTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA1078090.10460627491135285No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)