FastQCFastQC Report
Thu 28 Sept 2023
SRR7808450.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7808450.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109837484
Total Bases5.4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAG1548885714.101613070452343No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA1303847811.87069980590597No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA33722663.070232380778132No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA30168422.746641574564836No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC25879602.3561719603846716No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC25039812.279714455221862No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC21029521.9146032150554357No Hit
ATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAAC18532621.6872764492675378Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC10413200.9480552194731628No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG8869720.8075312431592114No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC8650320.7875562772359207No Hit
CATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAA7924230.7214504294362751Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGA7627120.6944004653274832No Hit
CTTTTGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA6907940.628923728806461No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG6852320.623859883753346No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCAGATCGGAAG4932650.4490862154125817No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGATCGGAAGAG4756690.43306618348978204No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAG4573840.4164188611603667No Hit
ATTGGTCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAG4403060.4008704350875335No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCAC4297040.3912179925752851No Hit
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAGA4072450.37077051036602404No Hit
TTTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAG4027270.3666571605008724No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAA3861460.3515612211219259No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA3844610.35002713645552913No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA3767660.34302133140631663No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC3743430.3408153449691182No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACA2983340.27161401475656527No Hit
GCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT2850400.25951067852209725TruSeq Adapter, Index 12 (100% over 35bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTAGATCGGAA2657550.2419529201888856No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGCA2601270.23682898635952004No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGAGATC2500450.22764997056924577No Hit
ATTGATCATCGACACTTCGAACGAGATCGGAAGAGCACACGTCTGAACTC2329760.2121097384204467Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGAGATCGGAAG2017840.1837114185900325No Hit
CGGCGGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCGCCTCAGA1886810.1717819756322896No Hit
TCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAGATC1755840.1598579952905695No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCA1666120.15168956346450907No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAAGATCGGAAGA1542150.14040288832544637No Hit
GTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC1475200.13430751928003012No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGC1471520.1339724788306331No Hit
CTTTAGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA1465580.1334316798443781No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT1399150.12738365347115926No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGA1377290.12539344036686054No Hit
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGC1370530.12477798562829426No Hit
CGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCGCCTC1341450.12213043772925461No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG1267010.11535315211699496No Hit
GTTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAG1253820.11415228702798765No Hit
TTTGTTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC1241410.11302243594727643No Hit
ACATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGA1234720.1124133542607367Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAAGATC1223300.11137363634440134No Hit
TCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAAGATCG1209800.11014454773927633No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT1147710.1044916505917051No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGAGATCGGAAGAGC1140070.10379607748480474No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)