FastQCFastQC Report
Thu 28 Sept 2023
SRR7808451.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7808451.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119826521
Total Bases5.9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA115331319.6248567543741No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAG91672607.650443260386405No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC31332202.6147967694063317No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA22561681.8828619751048266No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA18196881.5186020463700185No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC17469711.4579168162614018No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC14466681.2073020128824403No Hit
ATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAAC13959881.1650075361864174Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCA9356030.7807979337061784No Hit
GCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC9352870.7805342191316729TruSeq Adapter, Index 5 (100% over 35bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCAGATCGGAAG7380050.615894539740497No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC7290100.6083878543048079No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG6304440.5261306050936754No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC5983890.49937943203742013No Hit
CATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAA5673930.47351203662167574Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAG5168420.43132521555891623No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA4596030.38355699236231683No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGA4520870.377284591280089No Hit
CTTTTGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA4278560.35706285756222533No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG3735360.3117306560206317No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG3720060.3104538101377407TruSeq Adapter, Index 5 (100% over 49bp)
CGGCGGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCGCCTCAGA3496270.2917776441160301No Hit
CGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCGCCTC3057410.2551530307718773No Hit
ATTGATCATCGACACTTCGAACGAGATCGGAAGAGCACACGTCTGAACTC2913410.24313565775643275Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTTGGTGACTCTAGATACCCTCGGGCCGATCGCACAGATCGGAAGAGCA2887300.24095667435759066No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGATCGGAAGAG2689720.22446783713264948No Hit
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAGA2644510.22069488272967552No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGA2597120.2167399986518844No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCAC2546000.2124738312314016No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAA2289130.19103700757530964No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAA2123220.1771911578739735No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCAC2014870.1681489192196442No Hit
GGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA2001630.1670439885340575Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATTGGTCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAG1989220.16600832465126814No Hit
GGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1869210.15599301259860496Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGGCGGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCGCCTCAGC1695390.1414870419212121No Hit
TGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAG1664410.13890163764330601Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAGG1572090.1311971662767377No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC1471650.1228150485984651No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACA1437180.11993838993289306No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGAGATCGGAAG1395470.11645752445737785No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGAAGATCGGAAG1371220.11443376545998526No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA1301880.10864706653713162No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGCA1271140.1060816912142513No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT1261290.1052596695184032No Hit
CTTTAGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA1233480.10293881435479546No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCAGATCGGAAGAGCA1233290.10292295809873342No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)