FastQCFastQC Report
Thu 28 Sept 2023
SRR7808452.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7808452.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104275133
Total Bases5.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA1793878317.203318263808878No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAG1326554712.72167833149635No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC1088887210.442443645696429No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG16750311.6063570976217312No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA15606261.4966425408443258No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCA11518061.1045835827416302No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC10411710.9984844612952928No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGA8994630.8625862889093606No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCAGATCGGAAG8914180.8548711225331164No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA8437160.809124837078846No Hit
GCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT7621470.7309000507340518TruSeq Adapter, Index 18 (100% over 35bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC7216580.6920710424795142No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCAC7011460.67240000547398No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA6445270.6181023044103908No Hit
CTTTTGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA6375600.6114209415585209No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG5597510.5368020005306539No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAG4889040.46885962734758635No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC4828200.46302506274434574No Hit
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAGA4807070.4609986927564025No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCAGATCGGAAG4742770.45483231366389143No Hit
CTTTGGTGACTCTAGATACCCTCGGGCCGATCGCACAGATCGGAAGAGCA4506790.43220179829451766No Hit
TTTGTTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC3960310.379794289018073No Hit
ATTGGTCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAG2794130.2679574621113166No Hit
GTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC2551960.24473332486662952No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAA2297700.22034975491232411No Hit
ATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAAC2279350.21858998731749402Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA2148150.2060078887648122No Hit
CTTTAGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA2006770.19244952677260072No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC1991970.1910302046797677No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGAAGATCGGAAG1623930.1557351166361015No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCAC1556990.14931556117027442No Hit
TTTGGTGACTCTAGATAAACTCGGGCCGATCGCACAGATCGGAAGAGCAC1494320.1433054993082579No Hit
CGGCGGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCGCCTCAGA1491520.1430369789123165No Hit
TTTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAG1429310.13707103111534752No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCAC1375350.13189625948499148No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTCGTATG1268840.12168193542366425TruSeq Adapter, Index 19 (97% over 49bp)
CTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC1256130.12046304462637318No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAGATCGGAAGAGCAC1203590.11542445119681602No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAGATCGGAAGAGCA1184500.11359371749734427No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAA1172890.11248031685560161No Hit
TTTGATGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC1154690.11073493428198264No Hit
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAGG1136210.10896269966876955No Hit
GGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG1119910.10739952736382509Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG1100940.10558030168132224No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACA1074790.10307251298351257No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGAGATCGGAAG1060540.10170593596845376No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)